General Cell Based Assays
Cayman Chemical PAF Acetylhydrolase Assay Kit, 96 Well
PAF-AH catalyzes the hydrolysis of the potent biologically-active phospholipid PAF, generating inactive lyso-PAF. Cayman’s PAF-AH assay kit provides an accurate and convenient method for measurement of PAF-AH activity. The assay uses 2-thio PAF which serves as a substrate for PAF-AH. Upon hydrolysis of the acetyl thioester bond at the sn-2 position by PAF-AH, free thiols are detected using Ellman’s reagent.
Eurofins Discoverx PathHunter eXpress cyno CXCR1 HEK 293 β-Arrestin GPCR Assay, 2pl Kit. (93-0980E1CCP0M)
The PathHunter cyno CXCR1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. CXCL8/IL8) based activation of cyno CXCR1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged cyno CXCR1 and EA-tagged β-Arrestin-2. Activation of cyno CXCR1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Cynomolgus monkey version of this protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Meso Scale Discovery RPLEX HUM Influenza B NP ASY
The R-PLEX Human Influenza B NP Assay Kit from Meso Scale Diagnostics is a multiplex immunoassay that simultaneously detects influenza B nucleoprotein antigens in human nasopharyngeal swab specimens This assay utilizes electrochemiluminescence detection technology and provides quantitative results for influenza B in under 3 hours It is intended for research use only and not for use in diagnostic procedures The kit includes a 96-well plate coated with capture antibodies detection antibodies labeled with electrochemiluminescent tags assay diluent wash buffer read buffer and calibrators and controls to allow quantitative analysis of samples
Eurofins Discoverx AssayComplete Thawing Reagent T10, 100. (92-4110TR)
AssayComplete Thawing Reagent T10 is an optimized thawing reagent for revival and recovery of Eurofins DiscoverX cell lines. This reagent ensures optimal post-thaw viability, recovery and assay performance upon thawing. This product is provided as a bottle of 100 mL. Please refer to cell line specific datasheet to determine the recommended thawing reagent. For detailed product information, User manual and datasheet, visit www.discoverx.com/reagents.
Abcam HDAC substrate, colorimetric (Boc-Lys(Ac)-pNA), 25MG
MW 408.45 g/mol, Purity >98%. Colorimetric substrate for histone deacetylases (HDAC). For use in HDAC activity assays.
The product is subject to the following: Abcam Restricted Use Statement
Eurofins Discoverx PathHunter eXpress mCCR6 CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0694E2MCP2M)
The PathHunter mCCR6 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. CCL20/MIP-3α) based activation of mCCR6 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged mCCR6 and EA-tagged β-Arrestin-2. Activation of mCCR6 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Mouse version of this protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
ILEX LIFE SCIENCES LLC QuickZyme Sensitive Tissue Collagen Assay Kit, 96 Assays, 96-well plate format, colorimetric assay for low level collagen analysis (tissue, tissue homogenates samples)
QuickZyme Sensitive Tissue Collagen Assay Kit enables collagen analysis in small tissue samples with low collagen levels. The assay is based on quantitative colorimetric determination of hydroxyproline residues, obtained by acid hydrolysis of collagen. The assay allows quantitative measurement of all types of collagen, species independent. The only hydroxyproline-based assay which shows no matrix effect and no need for further dilution of hydrolysate. No drying of hydrolysate is needed, resulting in a fast and easy (96-well plate format) assay. Application: tissues with low collagen/hydroxyproline content (ex. liver). Sensitivity: 2.4 µM (0.3 µg/ml). Assay-time: < 2 hours. Collagen is a main component of extracellular matrix. Dysregulation in collagen production results in pathologies such as fibrosis (too much collagen), or osteoarthritis (too little collagen). Thus measurement of collagen production is important in many disease related studies.
Eurofins Discoverx PathHunter eXpress KDR/KDR Dimerization Assay, 2-Plate Kit. (93-0996E1CP0M)
The PathHunter KDR/KDR Dimerization eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This kit assesses ligand (e.g. VEGF-121) induced dimerization of KDR/KDR receptors. This product uses EFC technology. The cell line included overexpresses KDR target protein fused to PK alongside KDR target protein fused to EA. Upon ligand-induced dimerization of KDR/KDR, detectable EFC signal is produced. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
ABclonal Technology HRP-conjugated B-Actin
This gene encodes one of six different actin proteins Actins are highly conserved proteins that are involved in cell motility structure integrity and intercellular signaling The encoded protein is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins that are ubiquitously expressed Mutations in this gene cause Baraitser-Winter syndrome 1 which is characterized by intellectual disability with a distinctive facial appearance in human patients Numerous pseudogenes of this gene have been identified throughout the human genome
Eurofins Discoverx PathHunter eXpress PTGER2 CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0214E2CP2L)
The PathHunter PTGER2 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. prostaglandin E2) based activation of PTGER2 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged PTGER2 and EA-tagged β-Arrestin-2. Activation of PTGER2 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Revvity Health Sciences Inc HTRF Insulin High Range Detection Kit, 100,000 Assay Points
HTRF Insulin High Range Detection Kit, 100,000 Assay Points
Eurofins Discoverx PathHunter eXpress MC5R CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0231E2CP2M)
The PathHunter MC5R eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. α-MSH) based activation of MC5R GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged MC5R and EA-tagged β-Arrestin-2. Activation of MC5R stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx AssayComplete Cell Plating 10 Reagent, 100. (93-0563R10A)
AssayComplete Cell Plating 10 Reagent is an optimized cell plating reagent for use in plating of Eurofins DiscoverX cell lines for assays. This reagent was optimized for maximal cell health and ligand responsiveness during assays while eliminating hormones and growth components which may lead to increased basal activity. This product is provided as a bottle of 100 mL. Please refer to cell line specific datasheet to determine the recommended cell plating reagent. For detailed product information, User manual and datasheet, visit www.discoverx.com/reagents.
Eurofins Discoverx PathHunter eXpress GPR107 CHO-K1 β-Arrestin Orphan GPCR Assay, 2-Plate Kit. (93-0419E2ACP1M)
The PathHunter GPR107 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of GPR107 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR107 and EA-tagged β-Arrestin-2. Activation of GPR107 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. This is an orphan receptor without a known ligand. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx AssayComplete Thawing Reagent T4, 100. (92-4104TR)
AssayComplete Thawing Reagent T4 is an optimized thawing reagent for revival and recovery of Eurofins DiscoverX cell lines. This reagent ensures optimal post-thaw viability, recovery and assay performance upon thawing. This product is provided as a bottle of 100 mL. Please refer to cell line specific datasheet to determine the recommended thawing reagent. For detailed product information, User manual and datasheet, visit www.discoverx.com/reagents.