General Cell Based Assays
Eurofins Discoverx PathHunter eXpress ACVR1C/ACVR2B Dimerization Assay, 2pl Kit. (93-1047E3CP5M)
The PathHunter ACVR1C/ACVR2B Dimerization eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This kit assesses ligand (e.g. activin B) induced dimerization of ACVR1C/ACVR2B receptors. This product uses EFC technology. The cell line included overexpresses ACVR2B target protein fused to PK alongside ACVR1C target protein fused to EA. Upon ligand-induced dimerization of ACVR1C/ACVR2B, detectable EFC signal is produced. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress Flt4 Activity Assay, 2-Plate Kit. (93-0888E3CP16M)
The PathHunter FLT4 Activity eXpress kit provides an easy-to-use cell based assay to study small molecule drug candidates targeting RTKs. This kit assesses RTK activity via detection of resulting FLT4 phosphorylation-induced SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses ProLink-tagged FLT4 to the extracellular ligand binding domain of EGFR alongside an EA-tagged SH2 domain protein. Upon ligand induced receptor activation, cross-phosphorylation of the receptor leads to SH2-EA recruitment and production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress rhTrkA-rhP75 Functional Assay, 2pl Kit. (93-1009E3CP16M)
The PathHunter eXpress rhTrkA-rhP75 Functional Assay provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting RTKs. This kit assesses RTK activation via detection of activated rhTrkA and rhP75 dimerization-induced SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses ProLink-tagged rhTrkA and an EA-tagged SH2 domain protein. It also overexpresses an untagged coreceptor, rhP75 Upon ligand-induced receptor activation, SH2-EA recruitment leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the rhesus version of the protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress TGFBR1/ACVR2 Dimerization Assay, 10pl Kit. (93-1012E3CP5L)
The PathHunter TGFBR1/ACVR2 Dimerization eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This kit assesses ligand (e.g. GDF-11) induced dimerization of TGFBR1/ACVR2 receptors. This product uses EFC technology. The cell line included overexpresses TGFBR1 target protein fused to PK alongside ACVR2 target protein fused to EA. Upon ligand-induced dimerization of TGFBR1/ACVR2, detectable EFC signal is produced. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress rhTrkA-rhP75 Functional Assay, 10pl Kit. (93-1009E3CP16L)
The PathHunter eXpress rhTrkA-rhP75 Functional Assay provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting RTKs. This kit assesses RTK activation via detection of activated rhTrkA and rhP75 dimerization-induced SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses ProLink-tagged rhTrkA and an EA-tagged SH2 domain protein. It also overexpresses an untagged coreceptor, rhP75 Upon ligand-induced receptor activation, SH2-EA recruitment leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the rhesus version of the protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter OSMRb/IL31R Bioassay Kit, 2-Plate. (93-1002Y3-00083)
The PathHunter OSMRb/IL31R Dimerization Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses ligand (e.g. IL-31) induced dimerization of OSMRb/IL31R receptors. This product uses EFC technology. The cell line included overexpresses OSMRb target protein fused to PK alongside IL31RA target protein fused to EA. Upon ligand-induced dimerization of OSMRb/IL31R, detectable EFC signal is produced. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.
Eurofins Discoverx PathHunter eXpress mINSRb Functional Assay, 10-Plate Kit. (93-1017E3CP16L)
The PathHunter eXpress mINSRb Functional Assay provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting RTKs. This kit assesses RTK activation via detection of activated mINSRb dimerization-induced SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses ProLink-tagged mINSRb and an EA-tagged SH2 domain protein. Upon ligand-induced receptor activation, SH2-EA recruitment leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Mouse version of the protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress cINSRa Functional Assay, 2-Plate Kit. (93-1016E3CP16M)
The PathHunter eXpress cINSRa Functional Assay provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting RTKs. This kit assesses RTK activation via detection of activated cINSRa dimerization-induced SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses ProLink-tagged cINSRa and an EA-tagged SH2 domain protein. Upon ligand-induced receptor activation, SH2-EA recruitment leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Cynomolgus monkey version of the protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress rTrkA-rP75 Functional Assay, 2-Plate Kit. (93-1014E3CP16M)
The PathHunter eXpress rTrkA-rP75 Functional Assay provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting RTKs. This kit assesses RTK activation via detection of activated rTrkA and rP75 dimerization-induced SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses ProLink-tagged rTrkA and an EA-tagged SH2 domain protein. It also overexpresses an untagged coreceptor, rP75 Upon ligand-induced receptor activation, SH2-EA recruitment leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Rat version of the protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress IFNRL1/IL10RB Dimerization Assay, 2pl Kit. (93-0993E3CP5M)
The PathHunter IFNRL1/IL10RB Dimerization eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This kit assesses ligand (e.g. IL-29) induced dimerization of IFNRL1/IL10RB receptors. This product uses EFC technology. The cell line included overexpresses IFNRL1 target protein fused to PK alongside IL10RB target protein fused to EA. Upon ligand-induced dimerization of IFNRL1/IL10RB, detectable EFC signal is produced. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress BMPR1A/ACVR2 Dimerization Assay, 2pl Kit. (93-1037E3CP5M)
The PathHunter BMPR1A/ACVR2 Dimerization eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This kit assesses ligand (e.g. BMP-4) induced dimerization of BMPR1A/ACVR2 receptors. This product uses EFC technology. The cell line included overexpresses BMPR1A target protein fused to PK alongside ACVR2 target protein fused to EA. Upon ligand-induced dimerization of BMPR1A/ACVR2, detectable EFC signal is produced. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter IL10RA/10RB Dimerization Bioassay Kit, 10pl. (93-0985Y3-00136)
The PathHunter IL10RA/IL10RB Dimerization Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses ligand (e.g. IL-10) induced dimerization of IL10RA/IL10RB receptors. This product uses EFC technology. The cell line included overexpresses IL10RA target protein fused to PK alongside IL10RB target protein fused to EA. Upon ligand-induced dimerization of IL10RA/IL10RB, detectable EFC signal is produced. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.
Eurofins Discoverx PathHunter eXpress GALR2 CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0759E2CP2L)
The PathHunter GALR2 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. galanin) based activation of GALR2 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GALR2 and EA-tagged β-Arrestin-2. Activation of GALR2 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress C5AR1 Activated GPCR Internalization Assay, 2pl Kit. (93-0777E3CP5M)
The PathHunter C5AR1 Activated GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of C5AR1 via detection of localization to the early endosome. The included cell line overexpresses EA-tagged β-Arrestin-2 as well as a ProLink-tagged early endosome protein and untagged C5AR1. Upon ligand-induced receptor activation, β-Arrestin-2 is recruited to the activated GPCR and the resulting C5AR1-arrestin complex is internalized to the early endosome, leading to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress PROKR1 Total GPCR Internalization Assay, 2pl Kit. (93-0764E3CP0M)
The PathHunter PROKR1 Total GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of PROKR1 via detection of localization to the early endosome. The included cell line overexpresses ProLink-tagged PROKR1 and an EA-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the PROKR1 receptor to the early endosome leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.