General Cell Based Assays
Eurofins Discoverx PathHunter eXpress TrkC-P75 Functional Assay, 2-Plate Kit. (93-0531E3CP16M)
The PathHunter eXpress TrkC-P75 Functional Assay provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting RTKs. This kit assesses RTK activation via detection of activated TrkC and P75 dimerization-induced SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses ProLink-tagged TrkC and an EA-tagged SH2 domain protein. It also overexpresses an untagged coreceptor, P75. Upon ligand-induced receptor activation, SH2-EA recruitment leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress TrkB-P75 Functional Assay, 10-Plate Kit. (93-0530E3CP16L)
The PathHunter eXpress TrkB-P75 Functional Assay provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting RTKs. This kit assesses RTK activation via detection of activated TrkB and P75 dimerization-induced SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses ProLink-tagged TrkB and an EA-tagged SH2 domain protein. It also overexpresses an untagged coreceptor, P75. Upon ligand-induced receptor activation, SH2-EA recruitment leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress ErbB2-ErbB3 Functional Assay, 2-Plate Kit. (93-0535E3CP16M)
The PathHunter eXpress ErbB2-ErbB3 Functional Assay provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting RTKs. This kit assesses RTK activation via detection of activated ErbB2 and ErbB3 dimerization-induced SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses ProLink-tagged ErbB2 and an EA-tagged SH2 domain protein. It also overexpresses an untagged coreceptor, ErbB3 Upon ligand-induced receptor activation, SH2-EA recruitment leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress GPR18 CHO-K1 β-Arrestin Orphan GPCR Assay, 2-Plate Kit. (93-0560E2ACP1M)
The PathHunter GPR18 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of GPR18 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR18 and EA-tagged β-Arrestin-2. Activation of GPR18 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. This is an orphan receptor without a known ligand. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress mCCR7 CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0528PE2MCP0M)
The PathHunter mCCR7 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. CCL19/MIP-3β) based activation of mCCR7 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged mCCR7 and EA-tagged β-Arrestin-2. Activation of mCCR7 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Mouse version of this protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Meso Scale Discovery R-PLEX Human Neprilysin ASY
The R-Plex Human Neprilysin Assay from Meso Scale Discovery is a 96-well plate assay using electrochemiluminescence (ECL) for quantifying human neprilysin in biological samples It features a pre-coated plate with an ECL label-conjugated antibody specific for neprilysin Samples bind to the immobilized antibody and a ruthenylated detection antibody is added ECL is induced by a read buffer and measured with the MSD instrument with the signal proportional to neprilysin levels The assay offers high sensitivity a wide dynamic range and results in 90 minutes
Meso Scale Discovery U-PLEX Mouse IL-9 ASY
The Meso Scale Discovery U-PLEX Mouse IL-9 Assay Kit is a multiplex immunoassay that measures mouse interleukin 9 (IL-9) in serum plasma and cell culture supernatants It uses electrochemiluminescence (ECL) detection technology and provides high sensitivity up to 5 logs of dynamic range and low sample volume requirements This assay kit includes a 96-well plate coated with capture antibodies detection antibodies labeled with an ECL tag calibrators and diluents It can accurately quantify IL-9 concentrations from as low as 24 pg/mL to 50 000 pg/mL The assay takes 3 5 hours to complete including a 2 hour incubation step
Echelon Biosciences Research Labs PI(3 5)P2 DiC16 1 mg
PI(3,5)P2 diC16 9Phosphatidylinositol 3,5-bisphosphate diC16) is a synthetic, purified dipalmitoyl PI(3,5)P2.Phosphoinositides (PIPns) are minor components of cellular membranes but are integral signaling molecules for cellular communication. Phosphatidylinositol 3,5-bisphosphate is in very low abundance and is required for retrograde membrane trafficking from lysosomal and late endosomal compartments to the Golgi and is involved in autophagy. PI(3,5)P2 levels are controlled by the 5-kinase PIKfyve and the 5-phosphatase Fig4/Sac3 and disregulation is linked to several human neuropathies (eg Chacot-Marie-Tooth disease). This item is non returnable
Meso Scale Discovery Human/NHP IgG Kit, 1 plate
Immunoglobulins (Ig) are critical components of the immune system and play a major role in eliciting immune response against pathogens. Five heavy-chain isotypes (IgA, IgD, IgE, IgG, and IgM) mediate specific biological functions, including responses to pathogens and immunological diseases such as allergy and autoimmune disorders. IgG, a monomer, is the dominant immunoglobulin class present in serum. There are four subclasses of IgG, namely IgG1, IgG2, IgG3, and IgG4. IgG is commonly used as a diagnostic tool for humoral immunodeficiencies, autoimmune diseases, liver diseases, chronic inflammatory diseases, haematological disorders, infections, and lymphoid malignancies. This kit provides the assay-specific components for the quantitative determination of IgG in human, cynomolgus monkey, and rhesus monkey samples.
Eurofins Discoverx PathHunter eXpress CX3CR1 CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0956E2CP0L)
The PathHunter CX3CR1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. CX3CL1/fractalkine) based activation of CX3CR1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged CX3CR1 and EA-tagged β-Arrestin-2. Activation of CX3CR1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress ACVRL1/ACVR2B Dimerization Assay, 2pl Kit. (93-0964E3CP22M)
The PathHunter ACVRL1/ACVR2B Dimerization eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This kit assesses ligand (e.g. GDF2/BMP9) induced dimerization of ACVRL1/ACVR2B receptors. This product uses EFC technology. The cell line included overexpresses ACVRL1 target protein fused to PK alongside ACVR2B target protein fused to EA. Upon ligand-induced dimerization of ACVRL1/ACVR2B, detectable EFC signal is produced. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress ACVRL1/ACVR2 Dimerization Assay, 2pl Kit. (93-0963E3CP0M)
The PathHunter ACVRL1/ACVR2 Dimerization eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This kit assesses ligand (e.g. GDF2/BMP9) induced dimerization of ACVRL1/ACVR2 receptors. This product uses EFC technology. The cell line included overexpresses ACVR2 target protein fused to PK alongside ACVRL1 target protein fused to EA. Upon ligand-induced dimerization of ACVRL1/ACVR2, detectable EFC signal is produced. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress BRS3 CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0945E2CP2L)
The PathHunter BRS3 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. bombesin) based activation of BRS3 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged BRS3 and EA-tagged β-Arrestin-2. Activation of BRS3 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.