General Cell Based Assays
Eurofins Discoverx PathHunter Human c-Ret-GFR alpha 3 Functional Kinase Cell-Based Assay Kit (U2OS)
The PathHunter Human c-Ret-GFR alpha 3 Stable Cell Line Functional Kinase Assay (U2OS) measures c-Ret-GFRα3 receptor tyrosine kinase activity via ligand-induced SH2 domain protein recruitment. Cyno version. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter Human AMHR2/BMPR1A Dimerization Cell-Based Assay Kit (U2OS)
The PathHunter Human AMHR2/BMPR1A Dimerization Stable Cell Line Assay (U2OS) line ships as two vials of cryopreserved cells. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter Human FGFR1-beta-Klotho Functional Kinase Cell-Based Assay Kit (U2OS)
The PathHunter Human FGFR1-beta-Klotho Stable Cell Line Functional Kinase Assay (U2OS) measures FGFR1-β-Klotho receptor tyrosine kinase activity via ligand-induced SH2 domain protein recruitment. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter eXpress CCKAR CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0190E2CP2M)
The PathHunter CCKAR eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. cholecystokinin) based activation of CCKAR GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged CCKAR and EA-tagged β-Arrestin-2. Activation of CCKAR stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Revvity Health Sciences Inc HTRF Phospho-NDRG1 (Ser330) Detection Kit, 50,000 Assay Points
HTRF Phospho-NDRG1 (Ser330) Detection Kit, 50,000 Assay Points
Revvity Health Sciences Inc HTRF Human Phospho-STAT5 (Tyr694) Detection Kit, 50,000 Assay Points
HTRF Human Phospho-STAT5 (Tyr694) Detection Kit, 50,000 Assay Points
Eurofins Discoverx PathHunter eXpress GPR78 CHO-K1 β-Arrestin Orphan GPCR Assay, 10-Plate Kit. (93-0370E2ACP1L)
The PathHunter GPR78 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of GPR78 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR78 and EA-tagged β-Arrestin-2. Activation of GPR78 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. This is an orphan receptor without a known ligand. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Cayman Chemical MitoLite™ Orange EX405, 500 test
A mitochondrial dye for live cells; ex/em = 399/550 nm
Cayman Chemical cPLA2 Assay Kit, 96 Well
Arachidonoyl thio-PC is a substrate for cPLA2 by virtue of the presence of arachidonic acid at the sn-2 position of the glycerophospholipid. Hydrolysis of the arachidonoyl thioester bond at the sn-2 position by PLA2 releases free thiol which can be detected by DTNB. This assay can be used to determine the activity of cPLA2 in purified preparations, cell cultures, or tissue homogenates that are known to contain only cPLA2. Use of this assay with preparations containing more than one type of PLA2 will result in the measurement of total PLA2 activity rather than cPLA2 alone. Isozyme-specific cPLA2 activity can be measured by excluding sPLA2 or inhibiting iPLA2 activities in the assay.
Medchemexpress LLC PLAU/uPA Rat HEK29 10ug
PLAU/uPA proteins lack conserved residues critical for feature annotation propagation suggesting potential differences in molecular interactions in plasminogen activation and fibrinolysis regulation PLAU/uPA Protein Rat (HEK293 His) is the recombinant rat-derived PLAU/uPA protein expressed by HEK293 with C-His labeled tag
Discovery Life Sciences Gentest TransportoCellsTM
Gentest TransportoCellsTM Dog Organic Anion transporting Polypeptide 1b4 10e7 cells
Eurofins Discoverx PathHunter eXpress ACVR1C/ACVR2 Dimerization Assay, 2pl Kit. (93-1069E3CP5M)
The PathHunter ACVR1C/ACVR2 Dimerization eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This kit assesses ligand (e.g. activin B) induced dimerization of ACVR1C/ACVR2 receptors. This product uses EFC technology. The cell line included overexpresses ACVR1C target protein fused to PK alongside ACVR2 target protein fused to EA. Upon ligand-induced dimerization of ACVR1C/ACVR2, detectable EFC signal is produced. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx cAMP Hunter eXpress DRD2S (Short Isoform) CHO-K1 GPCR Assay, 2pl Kit. (95-0084E2CP2M)
The cAMP Hunter DRD2S eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. dopamine) based activation of DRD2S GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type DRD2S, a naturally Gi/Go coupled GPCR. Upon activation of DRD2S, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Cayman Chemical cGAS TR-FRET Inhibitor Screening Assay Kit, 96 Well
Cayman’s cGAS TR-FRET Inhibitor Screening Assay Kit provides a robust and easy-to-use time-resolved Förster resonance energy transfer (TR-FRET) platform for identifying novel inhibitors of human cGAS, which is a cystosolic dsDNA sensor that detects microbial pathogens. This assay utilizes a mouse anti-rabbit monoclonal immunoglobulin G (IgG) directly labeled with a europium (Eu3+) chelate as a donor molecule and fluorescently labeled 2’3’-cGAMP as the acceptor molecule. In the presence of an anti-2’3’-cGAMP antibody, the donor and acceptor are brought into close proximity resulting in a FRET from the donor to the acceptor upon excitation of the Eu3+-conjugated IgG at 340 nm and an emission from the acceptor at 665 nm. In the assay, production of 2’3’-cGAMP by cGAS in the presence of dsDNA, ATP, and GTP results in the displacement of fluorescently labeled 2’3’-cGAMP from the anti-2’3’-cGAMP antibody, resulting in a loss of.