General Cell Based Assays
Bioassay Systems EnzyChrom™ Ascorbic Acid Assay Kit. For quantitative determination of ascorbate (ascorbic acid) and evaluation of drug effects on ascorbic acid metabolism.
For quantitative determination of ascorbate (ascorbic acid) and evaluation of drug effects on ascorbic acid metabolism. Key Features: Use 20 µL samples. Linear detection range: colorimetric assay 6 to 1000 µM, fluorimetric assay 1 to 100 µM ascorbic acid. Method: OD570nm, or FL530/585nm. Samples: Serum, plasma, urine, saliva, milk, tissue, and cell culture. Species: All. Procedure: Assay takes 10 min. Kit size: 100 tests. Detection limit: OD, FL: 6, 1 µM.
Bioassay Systems EnzyFluo™ AMPK Phosphorylation Assay Kit. For quantitative fluorescent immunoenzymatic assay of AMPK phosphorylation status in cultured cells.
For quantitative fluorescent immunoenzymatic assay of AMPK phosphorylation status in cultured cells. Key Features: Sensitive. Can measure pAMPK modulation in as little as 500 cells/well. New and improved. Total assay time reduced from the standard 21 hours to 6.5 hours (hands-on time 2.5 hrs). Simple and convenient. Cells are directly cultured in 96-well plates. No cell lysis necessary. Accurate and high-throughput. Protein phosphorylation is normalized to total cellular protein in the same well, greatly minimizing well-to-well variations. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: FL360/450nm, 530/585nm. Samples: Cells. Species: Human, mouse, rat. Procedure: Assay takes Assay takes 6.5 hrs, hands-on time 2.5 hrs. Kit size: 100 tests.
Dojindo Molecular Technologies Inc DNA DAMAGE QUANTIFIC KIT (20 SAMPLES) is a colorimetric microplate assay and can determine the number of abasic sites in genomic DNA samples.
Oxidative damage to DNA is a result of its interaction with reactive oxygen species (ROS), in particular the hydroxy radical. Hydroxy radicals, which are produced from superoxide anion and hydrogen peroxide by the Fenton reaction, produce multiple modifications in DNA. Oxidative attacks by hydroxy radicals on the deoxyribose moiety leads to the release of free bases from DNA, generating strand breaks with various sugar modifications and simple abasic sites (AP sites). In fact, AP sites are one of the major types of damage generated by ROS. The Aldehyde Reactive Probe reacts specifically with an aldehyde group present on the open ring form of the AP sites. DNA Damage Quantification Kit contains all the necessary solutions for detecting between 1 to 40 AP sites per 1 x 105 base pairs.
Biochain Institute Inc NAD/NADH Assay Kit, 100 Assays/PK
BioChain's NAD+/NADH assay kit provides simple, direct and automation-ready procedure to measure NAD+/NADH concentration. It based on an alcohol dehydrogenase cycling reaction, in which a tetrazolium dye (MTT) is reduced by NADH in the presence of phenazine methosulfate (PMS). The intensity of the product color, measured at 565 nm, is proportionate to the NAD+/NADH concentration in the sample.
Bioassay Systems EnzyChrom™ Pyruvate Assay Kit. For quantitative determination of pyruvate and evaluation of drug effects on its metabolism.
For quantitative determination of pyruvate and evaluation of drug effects on its metabolism. Key Features: Sensitive and accurate. Use as little as 10 µL samples. Linear detection range in 96-well plate: 2 to 500 µM (17 µg/dL to 4.4 mg/dL) pyruvate for colorimetric assays and 0.2 to 50 µM for fluorimetric assays. Simple and convenient. The procedure involves addition of a single working reagent and incubation for 30 min at room temperature, compatible for HTS assays. Improved reagent stability. The optimized formulation has greatly enhanced the reagent and signal stability. Method: OD570nm, or FL530/585nm. Samples: Biological. Species: All. Procedure: Assay takes 20 min. Kit size: 100 tests. Detection limit: OD, FL: 2, 0.2 µM.
Bioassay Systems PiBlue™ Phosphate Assay Kit. For sensitive and high-throughput phosphate determination.
For sensitive and high-throughput phosphate determination. Key Features: Reagent very stable. Due to our innovative formulation, no precipitation of reagent occurs. Therefore no filtration of reagent is needed prior to assays, as is often required with other commercial kits. High sensitivity and wide detection range: detection of as little of 20 pmoles of phosphate and useful range between 0.4 µM and 50 µM phosphate. Fast and convenient: single reagent "mix-and-measure". Compatible with routine laboratory and HTS formats: assays can be performed in tubes, cuvettes or microplates, on spectrophotometers and plate readers. Robust and amenable to HTS: Z factors of 0.7 to 0.9 are observed in 96-well plates. Can be readily automated on HTS liquid handling systems Method: OD620nm (malachite green). Samples: Enzyme reactions with free phosphate releases. Species: All. Procedure: Assay takes 30 min. Kit size: 500 tests. Detection limit: 0.4 µM.
Eurofins Discoverx HitHunter cAMP Assay for Biologics, 2,500/10,000 dp (96-well/384-well). (90-0075LM25)
This detection kit is a robust, sensitive and easy-to-use assay to determine GPCR activity through detection of intracellular cAMP levels. The HitHunter cAMP assay is intended for use with cells expressing Gαs- or Gαi-coupled GPCRs. This product uses EFC technology, and contains ED-tagged cAMP, anti-cAMP antibody, and pure EA. Upon application to a cell which contains endogenous, untagged cAMP, the anti-cAMP antibody will be sequestered by binding to this untagged cAMP, allowing for EFC between the tagged cAMP and soluble EA. This will produce a proportional level of chemiluminescent signal. This kit format is intended for Biologic drugs with a protocol optimized for use in a 96-well plate. This kit may be used with Eurofins DiscoverX cAMP cell lines as well as cell lines from other sources. This detection kit contains reagents for assaying 25 plates (96-well). For detailed product information, visit www.discoverx.com/products-applications/biochemical-assays.
ABclonal Technology HRP-conjugated B-Actin
This gene encodes one of six different actin proteins Actins are highly conserved proteins that are involved in cell motility structure integrity and intercellular signaling The encoded protein is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins that are ubiquitously expressed Mutations in this gene cause Baraitser-Winter syndrome 1 which is characterized by intellectual disability with a distinctive facial appearance in human patients Numerous pseudogenes of this gene have been identified throughout the human genome