General Cell Based Assays
Abcam Pyruvate Kinase Assay Kit
Pyruvate Kinase Assay Kit
The product is subject to the following: Abcam Restricted Use Statement
Abcam ADP Assay Kit (Colorimetric/Fluorometric)
ADP Assay Kit (Colorimetric/Fluorometric)
The product is subject to the following: Abcam Restricted Use Statement
Abcam Sialic Acid (NANA) Assay Kit
Sialic Acid (NANA) Assay Kit
The product is subject to the following: Abcam Restricted Use Statement
Abcam Starch Assay Kit
Starch Assay Kit
The product is subject to the following: Abcam Restricted Use Statement
Abcam Paraoxonase 1 Activity Assay Kit
Paraoxonase 1 Activity Assay Kit
The product is subject to the following: Abcam Restricted Use Statement
Indigo Biosciences Human RORa Reporter Assay system 1x 384- well format assays
Human RORa Reporter Assay system 1x 384- well format assays
Abcam Swinholide A, F-actin inhibitor, 10UG
MW 1389.9 Da, Purity >97%. Cell permeable, selective F-actin inhibitor. Sequesters and stabilizes actin dimers. Induces actin depolymerization and apoptosis. .
The product is subject to the following: Abcam Restricted Use Statement
Cayman Chemical ERK MAPK PhosphoThr202 Tyr20
Antigen: phosphopeptide corresponding to amino acid residues surrounding the phospho-Thr202 and phospho-Tyr204 of rat ERK/MAPK • Host: rabbit • Cross Reactivity: (+) human and rat ERK/MAPK • Application(s): WB • ERK/MAPK is an integral component of cellular signaling during mitogenesis and differentiation of mitotic cells and also is thought to play a key role in learning and memory. The activity of this kinase is regulated by dual phosphorylation at Thr202 and Tyr204.
Indigo Biosciences Human Pregnane X Receptor (PXR, NR1I2) All-inclusive Cell-Based Luciferase Reporter Assay Kit, 384-well assay plate format
Eliminate weeks of cell culture while achieving superior sensitivity with reproducible results from this all-inclusive cell-based luciferase reporter assay. INDIGO’s Human Pregnane X Receptor reporter assay allows you to quickly screen test samples to quantify functional activity, either agonist or antagonist, they may exert against PXR (NR1I2). Kit includes cryopreserved reporter cells engineered to provide constitutive expression of the receptor, optimized media for use during cell thawing and in diluting test samples, reference agonist, Luciferase Detection Reagent, cell culture-ready assay plate, and detailed assay protocol. INDIGO’s unique Cryomite™ process provides exceptional cell viability post-thaw and INDIGO’s strict QA process means consistent results lot to lot (product quality data available upon request). This kit allows researchers to achieve superior sensitivity, clear, reproducible results, and eliminate weeks of cell culture work.
Cayman Chemical HAT Inhibitor Screening Assay
Cayman’s HAT Inhibitor Screening Assay provides a fast, fluorescence-based method for evaluating PCAF HAT inhibitors. The procedure requires only three EAsy steps, all performed in the same microplate. In the first step of the protocol, HAT is incubated with acetyl-CoA and the histone H3 peptide. During this time, HAT catalyzes the enzymatic transfer of acetyl groups from acetyl-CoA to the H3 peptide producing an acetylated peptide and CoASH. Following addition of isopropanol to stop the enzymatic reaction, CPM is added to the wells of the plate. CPM reacts with the free thiol groups present on CoASH forming a highly fluorescent product that is detected using excitation and emission wavelengths of 360-390 and 450-470 nm, respectively.
Apexbio Technology LLC LDH Cytotoxicity Assay Kit(Synonyms: LDH Cytotoxicity Detection Kit, LDH Release Assay Kit, Lactate Dehydrogenase Cytotoxicity Assay), 1000T.
The LDH Cytotoxicity Assay Kit is a biochemical assay designed to quantitatively assess cell cytotoxicity through measurement of lactate dehydrogenase (LDH) enzyme activity released into the cell culture medium LDH an intracellular enzyme ubiquitously expressed is released extracellularly upon damage or death of the cell membrane This assay relies on LDH-mediated conversion of lactate to pyruvate coupled with NAD reduction to NADH The reaction product NADH interacts with specific substrates within the kit generating a compound measurable spectrophotometrically at 490 nm Accordingly absorbance intensity at this wavelength directly correlates with cellular damage severity Compared to radioactive chromium ( 51Cr) release assays this method provides comparable quantitative data in a safer non-radioactive format applicable for cell death cytotoxicity and drug screening research
