General Cell Based Assays
Eurofins Discoverx PathHunter SIRPa Signaling Bioassay Kit, 2pl. (93-1135Y19-00129)
The PathHunter SIRPa Signaling Bioassay consists of human cells engineered to measure drug potency and detect neutralizing antibodies. Engagement of the receptor by its ligand, or agonist antibodies results in phosphorylation of the receptor’s cytosolic tail. The SH2-domain fused to EA binds the phosphorylated receptor, forcing complementation of ED and EA, resulting in formation of an active β-gal enzyme, which hydrolyzes the substrate to generate a chemiluminescent signal. The bioassay kit contains all reagents needed for the assay including pre-qualified cryopreserved cells, detection reagents, cell plating reagent, positive control agonist, and assay plates. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.
Revvity Health Sciences Inc HTRF Human α-SMA Detection Kit, 500 Assay Points
HTRF Human α-SMA Detection Kit, 500 Assay Points
Sigma Aldrich Fine Chemicals Biosciences Bile Acid Assay Kit suffic
Twelve different types of bile acids are typically found in mammals among them two primary types are cholic acid and chenodeoxycholic acid. These can be dehydroxylated into secondary bile acids. finally these four can be conjugated to either taurine or glycine creating 8 different conjugated bile acids. Bile acid levels in feces blood urine and bile can be used as markers for various diseases such as hyperlipidemia cholestasis gall stones colon cancer etc. Bile acids also exist as sulfate salt forms known as bile acid sulfates. Sulfation of bile acids increases their solubility and decreases intestinal absorption thereby enhancing fecal and urinary excretion.
STEMCELL Technologies Cell NavigatorTM Golgi Stain
Kit containing C6 NBD ceramide for selectively staining the Golgi apparatus in live or aldehyde-fixed cells - 100 Tests
BioVendor, LLC QuickZyme Sensitive Tissue Hydroxyproline Kit 2 X 96-Assays (for collagen analysis)
QuickZyme Sensitive Tissue Hydroxyproline Kit, 2 X 96-Assays. Measure hydroxyproline in small tissue samples with low collagen levels! The ONLY hydroxyproline-based assay which shows no matrix effect and no need for further dilution of the hydrolysate, enabling measurement in small samples with low hydroxyproline levels. No drying of hydrolysate is needed, resulting in a fast (<2 hrs), sensitive and easy (96-well plate format) assay. Dynamic range: 6-300 uM. Sensitivity: 0.3 ug/ml. Species independent. Measures total hydroxyproline. If this hydroxyproline is obtained upon hydrolysis of collagen, it represents all collagen types present without discriminating between types of collagen, procollagen, mature collagen and collagen degradation products. Hydroxyproline in tissue hydrolysates can be used as a direct measure of the amount of collagen present. Collagen hydrolysis tubes & protocol not included (if these items are needed, please order QuickZyme Sensitive Tissue Collagen Assay).
Eurofins Discoverx PathHunter Bioassay Kit ED Detection Kit, 50 (1,000 dp, 96-well). (93-1043)
The PathHunter Bioassay ED Detection Kit is a detection kit intended for use with Eurofins DiscoverX's PathHunter Bioassay cells overexpressing ED (ProLabel/PK) but not containing any EA. This kit contains lysis buffer, exogenous EA, and EFC substrate to allow for detection of levels of ED in the assayed cells. This kit contains all components required to assay 10 plates (96-well). For detailed product information, User manual and COA, visit www.discoverx.com/products/reagents/detection-kit-93-1043.
Eurofins Discoverx PathHunter Mouse S1P1 (EDG1) β-Arrestin Cell-Based Assay Kit (CHO-K1)
The PathHunter Mouse S1P1 (EDG1) β-Arrestin Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure EDG1 (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter Human THR beta Nuclear Receptor Protein Interaction Cell-Based Assay Kit (CHO-K1)
The PathHunter Human THR beta Nuclear Receptor Stable Cell Line Protein Interaction Assay (CHO-K1) contains a stable clonal cell line used to measure activated THRβ nuclear hormone receptor interaction with SRCPs. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
American Research Products Inc Myc (Phospho-Thr58) Transcription Factor Activity Assay
Myc (Phospho-Thr58) Transcription Factor Activity Assay
Proteintech Group Inc Viability/Cytotoxicity Assay Kit for Animal Live & Dead Cells (Calcein AM, EthD-1 Method)
Viability/Cytotoxicity Assay Kit for Animal Live & Dead Cells (Calcein AM, EthD-I method) provides dual fluorescence staining for the detection of living and dead cells. The two probes in the kit eflect cell viability by measuring intracellular esterase activity and plasma membrane integrity. This kit can be used for fluorescence microscopes, flow cytometers, microplate readers and other fluorescence detection systems. This kit can be used for most eukaryotic mammalian samples, but not to fungi and yeast.
Eurofins Discoverx PathHunter eXpress ADCYAP1R1 Total Internalization Assay, 10-plate Kit. (93-0885E3CP0L)
The PathHunter ADCYAP1R1 Total Internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of ADCYAP1R1 via detection of localization to the early endosome. The included cell line overexpresses ProLink-tagged ADCYAP1R1 and an EA-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the ADCYAP1R1 receptor to the early endosome leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Cayman Chemical 8-propylthio NAD sodium sal
A derivative of NAD+; has been used in the screening of analog-sensitive PARP1 gatekeeper mutations
Eurofins Discoverx cAMP Hunter eXpress MCHR1 CHO-K1 GPCR Assay, 2-Plate Kit. (95-0055E2CP2M)
The cAMP Hunter MCHR1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. melanin concentrating hormone) based activation of MCHR1 GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type MCHR1, a naturally Gi/Go coupled GPCR. Upon activation of MCHR1, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Cayman Chemical Methyltrnsferase asy AdoHc 1ea
An ago-PAM of GLP-1R induces calcium mobilization in 9-3-H cells expressing human GLP-1R (EC50 1.3 UM) enhances GLP-1- exenatide- or liraglutide-induced calcium mobilization in the same cells (EC50s 1.8 8.4 and 30 UM respectively) potentiates exenatide-induced insulin secretion in primary mouse pancreatic islets at 30 UM reverses haloperidol-induced catalepsy in a rat model of Parkinson’s disease motor symptoms at 30 mg/kg
Dojindo Molecular Technologies Inc Liperfluo(50UGx5) is used to monitor lipid peroxidation in ferroptosis research, emitting intense fluorescence by a lipid peroxide specific oxidation, and is applicable for microscopy and FCM analysis
Liperfluo is designed and exclusively developed by Dojindo for detection of lipid peroxides. Among fluorescent probes that detect Reactive Oxygen Species (ROS), Liperfluo is the only compound that can specifically detect lipid peroxides. Since the excitation and emission wavelengths of the oxidized Liperfluo are 524 nm and 535 nm, respectively, both photo-damage and auto-fluorescence can be minimized. The tetraethyleneglycol group linked to one end of the diisoquinoline ring helps its solubility and dispersibility to aqueous buffer. Though Liperfluo's oxidized form is almost nonfluorescent in an aqueous media, it emits fluorescence in lipophilic sites such as cell membranes. Therefore, it can easily be applied to lipid peroxide imaging by a fluorescence microscopy and a flow cytometric analysis for living cells. Liperfluo is widely used in ferroptosis research, a research field Dojindo has a strong portfolio in. (https://www.dojindo.com/ferroptosis-detection-selection-guide/)