General Cell Based Assays
Eurofins Discoverx PathHunter eXpress XBP1 Nuclear Translocation Assay, 10pl Kit. (93-0857E3CP5L)
The PathHunter eXpress XBP1 Nuclear Translocation Assay provides an easy-to-use cell based assay to study drug candidates affecting the IRE1/XBP1 pathway. This kit assesses ligand (e.g. thapsigargin) based pathway activation via detection of resulting XBP1 nuclear translocation. This product uses EFC technology. The included cell line overexpresses ProLabel-tagged XBP1 and nuclear localized EA. Upon pathway activation, induced nuclear translocation of XBP1 leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Medchemexpress LLC cGAS Human His-SUM 100ug
Recombinant human cGAS (fragment G161-F522) expressed in E. coli with N-terminal 6xHis and SUMO tags. Supplied lyophilized with >90% purity and formulated in Tris-HCl or PBS with trehalose, this protein is suitable for biochemical and binding studies of cytosolic DNA sensing and cGAMP synthesis. Store lyophilized at -20°C; after reconstitution it is stable at 4°C for one week or at -20°C for extended storage when aliquoted.
Eurofins Discoverx PathHunter OSMRb/IL31R Bioassay Kit, 10-Plate. (93-1002Y3-00084)
The PathHunter OSMRb/IL31R Dimerization Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses ligand (e.g. IL-31) induced dimerization of OSMRb/IL31R receptors. This product uses EFC technology. The cell line included overexpresses OSMRb target protein fused to PK alongside IL31RA target protein fused to EA. Upon ligand-induced dimerization of OSMRb/IL31R, detectable EFC signal is produced. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.
Bioassay Systems EnzyChrom™ Histamine Assay Kit. For quantitative determination of histamine in food, beverage and agricultural products.
For quantitative determination of histamine in food, beverage and agricultural products. Key Features: Fast and sensitive. Use of 20 µL sample. Linear detection range 0.0025 - 1 mM (0.3 - 111 ppm) histamine in 96-well plate assay. Convenient. The procedure involves adding a single working reagent, and reading the absorbance after 30 minutes. Room temperature assay. No 37°C heater is needed. High-throughput. "Add-mix-read" type assay. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: OD565nm. Samples: Food, beverage and agricultural products. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 2.5 µM.
Eurofins Discoverx PathHunter eXpress rCHRM4 CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0870E2RCP2M)
The PathHunter rCHRM4 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. acetylcholine) based activation of rCHRM4 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged rCHRM4 and EA-tagged β-Arrestin-2. Activation of rCHRM4 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Rat version of this protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Meso Scale Discovery U-PLEX Human A2M ASY
The Meso Scale Discovery U-PLEX Human A2M Assay is a multiplex immunoassay kit that enables simultaneous quantitative measurement of Alpha-2-Macroglobulin (A2M) in human serum plasma and cell culture supernatant samples This electrochemiluminescent assay utilizes MSD s proprietary SECTOR Imager and provides high sensitivity a broad dynamic range and requires low sample volume The kit includes a 96-well plate pre-coated with capture antibodies detection antibodies and calibrators and controls needed to generate a standard curve and validate assay performance This assay allows rapid reproducible quantification of A2M which plays a key role in regulating protease activity
Eurofins Discoverx cAMP Hunter eXpress GRM4 CHO-K1 GPCR Assay, 2-Plate Kit. (95-0117E2CP24M)
The cAMP Hunter GRM4 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. L-glutamate) based activation of GRM4 GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type GRM4, a naturally Gi/Go coupled GPCR. Upon activation of GRM4, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Medchemexpress LLC cGAS Human His 5ug
cGAS is a cytosolic DNA sensor cGAS binds double-stranded DNA (dsDNA) and catalyzes the generation of cyclic guanosine-adenylate (cGAMP) which in turn activates the STING protein cGAS induces the production of type I interferon (IFN-I) and pro-inflammatory cytokines cGAS Protein Human (His) is a recombinant cGAS protein expressed in E coli with a C-6 His tag
Sigma Aldrich Fine Chemicals Biosciences SILuTMMAb K1 Pharmacokinet
All critical reagents necessary to perform the assay are provided in the kit. The plate-based format avoids the high cost and potential LC column blockage associated with bead-based enrichment formats. Sample preparation with the SILu(TM)MAb PK kit can be performed in under 5 hours and consists of coating wells with high-specificity capture antibody followed by immunoaffinity enrichment and rapid in-plate trypsin digestion. The resulting digest is ready for injection with no SPE or other cleanup required.
Eurofins Discoverx PathHunter eXpress CX3CR1 CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0956E2CP0M)
The PathHunter CX3CR1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. CX3CL1/fractalkine) based activation of CX3CR1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged CX3CR1 and EA-tagged β-Arrestin-2. Activation of CX3CR1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter MC4R Bioassay Kit, 2-Plate. (93-0211Y3-00085)
The PathHunter MC4R Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses ligand (e.g. αMSH) based activation of MC4R GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged MC4R and EA-tagged β-Arrestin-2. Activation of MC4R stimulates the recruitment of β-Arrestin-2 and produces EFC signal. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.
Eurofins Discoverx PathHunter eXpress mPTAFR CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0614E2MCP2M)
The PathHunter mPTAFR eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. PAF) based activation of mPTAFR GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged mPTAFR and EA-tagged β-Arrestin-2. Activation of mPTAFR stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Mouse version of this protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Meso Scale Discovery R-PLEX Human E-Cadherin ASY
The R-Plex Human E-Cadherin Assay from Meso Scale Diagnostics is a multiplex immunoassay that measures E-Cadherin levels in human serum plasma and cell culture supernatants It utilizes electrochemiluminescence detection technology and provides high sensitivity up to 5 logs of dynamic range and low sample volume requirements This assay allows for the quantitative measurement of E-Cadherin an important biomarker that plays a role in cell adhesion and is often monitored in cancer research With minimal hands-on time this robust and reproducible assay generates rapid results using automated instrumentation
Eurofins Discoverx PathHunter eXpress F2RL3 (PAR4) CHO-K1 β-Arrestin GPCR Assay, 10pl Kit. (93-0279E2CP0L)
The PathHunter F2RL3 (PAR4) eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. serine proteases) based activation of F2RL3 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged F2RL3 and EA-tagged β-Arrestin-2. Activation of F2RL3 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress PTHR1 CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0315E2CP0L)
The PathHunter PTHR1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. PTH) based activation of PTHR1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged PTHR1 and EA-tagged β-Arrestin-2. Activation of PTHR1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.