General Cell Based Assays
Eurofins Discoverx AssayComplete Cell Plating 43 Reagent, 100. (93-0563R43A)
AssayComplete Cell Plating 43 Reagent is an optimized cell plating reagent for use in plating of Eurofins DiscoverX cell lines for assays. This reagent was optimized for maximal cell health and ligand responsiveness during assays while eliminating hormones and growth components which may lead to increased basal activity. This product is provided as a bottle of 100 mL. Please refer to cell line specific datasheet to determine the recommended cell plating reagent. For detailed product information, User manual and datasheet, visit www.discoverx.com/reagents.
Eurofins Discoverx PathHunter eXpress ACVRL1/BMPR2 Dimerization Assay, 10pl Kit. (93-0962E3CP22L)
The PathHunter ACVRL1/BMPR2 Dimerization eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This kit assesses ligand (e.g. GDF2/BMP9) induced dimerization of ACVRL1/BMPR2 receptors. This product uses EFC technology. The cell line included overexpresses ACVRL1 target protein fused to PK alongside BMPR2 target protein fused to EA. Upon ligand-induced dimerization of ACVRL1/BMPR2, detectable EFC signal is produced. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx cAMP Hunter eXpress C5AR1 CHO-K1 GPCR Assay, 2-Plate Kit. (95-0079E2CP2M)
The cAMP Hunter C5AR1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. C5a) based activation of C5AR1 GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type C5AR1, a naturally Gi/Go coupled GPCR. Upon activation of C5AR1, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter Human NMBR Total GPCR Internalization Cell-Based Assay Kit (U2OS)
The PathHunter Human NMBR Stable Cell Line Total GPCR Internalization Assay (U2OS) contains a stable clonal cell line used to measure NMBR (GPCR) internalization via early-endosome localization. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx cAMP Hunter eXpress FFAR3 CHO-K1 GPCR Assay, 10-Plate Kit. (95-0136E2CP2L)
The cAMP Hunter FFAR3 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. FFAs) based activation of FFAR3 GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type FFAR3, a naturally Gi/Go coupled GPCR. Upon activation of FFAR3, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress ACVRL1/ACVR2 Dimerization Assay, 10pl Kit. (93-0963E3CP0L)
The PathHunter ACVRL1/ACVR2 Dimerization eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This kit assesses ligand (e.g. GDF2/BMP9) induced dimerization of ACVRL1/ACVR2 receptors. This product uses EFC technology. The cell line included overexpresses ACVR2 target protein fused to PK alongside ACVRL1 target protein fused to EA. Upon ligand-induced dimerization of ACVRL1/ACVR2, detectable EFC signal is produced. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress P2RY2 Activated GPCR Internalization Assay, 10pl Kit. (93-0719E3CP5L)
The PathHunter P2RY2 Activated GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of P2RY2 via detection of localization to the early endosome. The included cell line overexpresses EA-tagged β-Arrestin-2 as well as a ProLink-tagged early endosome protein and untagged P2RY2. Upon ligand-induced receptor activation, β-Arrestin-2 is recruited to the activated GPCR and the resulting P2RY2-arrestin complex is internalized to the early endosome, leading to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Meso Scale Discovery VPLEX Plus GLP1 Total
The Meso Scale Discovery V-PLEX Plus GLP-1 Total Kit is an electrochemiluminescent immunoassay for the quantitative measurement of total glucagon-like peptide-1 (GLP-1) in human serum plasma and culture media samples The kit contains a 96-well plate coated with a GLP-1 specific capture antibody a ruthenium-labeled detection antibody calibrators controls assay diluent read buffer wash buffer and plate seals The assay has a range from 0 61 pg/mL to 5000 pg/mL and takes approximately 3 5 hours to complete This product allows for the sensitive and accurate quantification of total GLP-1
Meso Scale Discovery U-PLEX Mouse IL-13 ASY
The Meso Scale Discovery U-PLEX Mouse IL-13 Assay Kit is a multiplex immunoassay for measuring mouse interleukin 13 (IL-13) in various biological samples It uses electrochemiluminescence (ECL) detection for high sensitivity and precision The kit includes a plate with capture antibodies ECL-tagged detection antibodies calibrators controls diluents buffers and seals It requires 50 L of sample per well and detects IL-13 from 0 05-1000 pg/mL with a total assay time of about 3 5 hours
Meso Scale Discovery R-PLEX Rat MIP-3 ASY
The R-PLEX Rat MIP-3alpha Assay Kit from Meso Scale Discovery is a multiplex immunoassay for measuring rat MIP-3alpha in various biological fluids It uses MSD s electrochemiluminescent detection for high sensitivity and a wide dynamic range with low sample volume An MSD instrument like QuickPlex is needed to read the plate The kit includes a 96-well plate with capture and detection antibodies plus calibrators and controls Detection limits are 1 52 pg/mL to 1500 pg/mL for rat MIP-3alpha
Cayman Chemical EstradIol 17 DGlucuronIde 50mg
An estrogen metabolite that acts as a substrate of MRP2 (Km = 75 µM), and through MRP2-mediated transport, functions as a cholestatic agent, decreasing bile flow
Eurofins Discoverx PathHunter Darbepoetin Alfa Bioassay Kit, 10pl. (93-0965Y3-00020)
The PathHunter Darbepoetin Alfa Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses erythropoetin or darbepoetin alfa-induced dimerization of EPOR receptors. This product uses EFC technology. The cell line included overexpresses EPOR target protein fused to PK alongside EPOR target protein fused to EA. Upon ligand-induced dimerization of EPOR, detectable EFC signal is produced. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.
Meso Scale Discovery U-PLEX Human MCP-3 Assay, Single-Analyte, 96-well plate, LLOD 0.79 pg/mL, Dynamic Range 0.79 - 5000 pg/mL, Uses human serum, plasma, cell culture supernatant
The U-PLEX Human MCP-3 Assay from MESO SCALE DISCOVERY offers a robust solution for the quantitative measurement of human Monocyte Chemoattractant Protein 3 (MCP-3/CCL7). This assay is designed for research applications in immunology and inflammation, providing sensitive detection across a wide dynamic range of human biological samples.
- High quality and ease of use with validated components
- Enables precise quantification of human MCP-3, crucial for inflammatory response
- Validated for diverse human sample types, including serum, plasma, and cell culture supernatant
- Features a low limit of detection at 0.79 pg/mL
- Wide dynamic range of 0.79 - 5,000 pg/mL for various concentrations
- Single-analyte format optimized for dedicated MCP-3 measurement
Cell Signaling Technology PathScanR Phospho-AMPK-alpha Thr172 Chemiluminescent Sandwich ELISA Kit 10 Kit
PathScanR Phospho-AMPK-alpha Thr172 Chemiluminescent Sandwich ELISA Kit 10 Kit
AnaSpec SensoLyte Red Glucocerebrosidase (GBA) Assay Kit *Fluorimetric*
The SensoLyte Red Glucocerebrosidase (GBA) Assay Kit detects GBA activity by using a highly sensitive fluorogenic substrate. The GBA substrate provided in the kit releases the red fluorescent dye resorufin upon Glucocerebrosidase cleavage with absorption/emission maxima at 570 nm/610 nm. This assay kit can be used to detect enzyme activity in purified enzyme preparations and compound screening. The Red Glucocerebrosidase (GBA) Assay is one-step homogenous reaction, which does not require the additional step of dispensing stop solution after the incubation, thereby suitable for HTS. The long wavelength fluorescence resorufin is also less interfered by the autofluorescence of components in biological samples and test compounds.