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Specialized protein assay reagents and kits to estimate and measure specific post-translational modifications (PTM) or functional (enzymatic) classes of proteins in biological samples, including glycoproteins, phosphoproteins, and proteases.
For rapid, direct determination of malic acid concentrations in food and beverage samples as well as biological samples. Key Features: Fast and sensitive. Use of 20 or 100 µL sample. Semi-quantitative measurement between 0-500 mg/L (undiluted) malic acid. Convenient. No expensive lab equipment needed. Sample trea™ent and assay can be performed in under 15 minutes. Method: Visual. Samples: Wine, beer, milk, etc. Species: All. Procedure: Assay takes approximately 15 min. Kit size: 10 tests.
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For quantitative colorimetric determination of glucose-6-phosphate concentration and evaluation of drug effects on its metabolism. Key Features: Fast and sensitive. Linear detection range (20 µL sample): 0.2 to 100 U/L for 15 min reaction. Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: OD460nm. Samples: Plasma, serum, tissue and culture media, etc. Species: All. Procedure: Assay takes 20 min. Kit size: 100 tests. Detection limit: 0.2 U/L.
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For quantitative determination of L-Lactate and screening for glycolysis modulators. Key Features: Fast and sensitive. Use of 5 µL sample. Linear detection range up to 10 mM L-lactate in 96-well plate assay. Convenient. The procedure involves adding a single working reagent, and reading the absorbance after 30 minutes. Room temperature assay. No 37°C heater is needed. High-throughput. "Add-mix-read" type assay. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: OD565nm. Samples: Cell media. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests.
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Reactive oxygen species ROS are natural byproducts of the normal metabolism of oxygen and play important roles in cell signaling. This catalog is for 200 Tests.
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For rapid quantitative determination of cytotoxicity based on lactate dehydrogenase released into cell culture medium. Evaluation of toxic compounds, toxins, detergents, environmental pollutants and physical trea™ent on cell lysis. Key Features: Safe. Non-radioactive assay (cf. chromium release assay). Fast. The whole procedure take 20 min. Robust and amenable to HTS. Single reagent, "mix-incubate-measure" type assay. High-throughput assay in 96-well plates allows simultaneous processing tens of thousands of samples per day. Method: OD500nm. Samples: Cell culture. Species: All. Procedure: Assay takes 20 min. Kit size: 100 tests.
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For quantitative determination of arginase activity and screen for its inhibitors. Key Features: Sensitive and accurate. Detection limit: 0.3 U/L for 2 hr arginase reaction in 96-well assay format. Simple and high-throughput. The procedure involves incubation of the provided substrate with the sample in a microplate followed by the addition of the coloring reagent. Can be readily automated as a high-throughput assay for thousands of samples per day. Method: OD430nm. Samples: Enzyme preparations, serum, plasma, tissue culture etc. Species: All. Procedure: Assay takes 2 hrs. Kit size: 100 tests. Detection limit: 1 U/L (0.3 U/L for 2 hr reaction).
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This kit contains recombinant TEV Protease and Control Substrate both expressed from E coli along with 10X Reaction Buffer All kit components are supplied in solution and can be used to cleave affinity tags from fusion proteins using the TEV protease recognition site Unit size 1000 U
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G6PDH Activity Colorimetric Assay kit provides a flexible, accurate, sensitive, and time-saving approach for detecting G6PDH activity in a wide variety of samples.
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Tobacco Etch Virus protease (TEV protease) is a highly sequence specific cysteine protease. It has a strict 7 amino acid cleavage recognition sequence of Glu-Asn-Leu-Tyr-Phe-Gln ↓ (Gly/Ser). The high specificity makes this protease excellent for the removal of affinity-tags from purified recombinant proteins. The kit contains a TEV protease substrate that is labeled with fluorophore FAM and a quencher. Proteolytic activity of TEV protease cleaves the substrate and releases the FAM, resulting in the production of bright fluorescence which can be measured using a fluorescence reader at ex/em of 490 nM/520 nm. TEV protease activity then can be calculated in accordance with the fluorescence intensity. Purified TEV protease is included in the kit as a positive control.
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