Protein Purification
Invent Biotechnologies Inc Minute™ Endosome Isolation and Cell Fractionation Kit - Spin Column-Based (20 Preps)
Early endosomes, which come mainly from primary endocytic vesicles that fuse with each other, provide the starting point for late endosome maturation. In addition to their roles in normal cell physiology, endocytic processes play a key role in many diseases such as Alzheimer’s disease and inherited lysosomal storage diseases. Traditional methods for isolating endosomes are based on density gradient ultracentrifugation. The protocol requires a large amount of starting material and is tedious and time-consuming. The Minute™ Endosome Isolation and Cell Fractionation Kit provides a spin-column-based novel endosome isolation technology that is rapid and simple and requires a smaller number of cultured cells or milligram amounts of tissues. This kit can precipitate and significantly enrich early endosomes from cultured cells or tissues. More Information: https://inventbiotech.com/products/minute-endosome-isolation-and-cell-fractionation-kit?_pos=1&_sid=fd15de666&_ss=r
STEMCELL Technologies EasySep™ Human Memory B Cell Isolation Kit, For processing: 2 x 10^9 cells
The EasySep™ Human Memory B Cell Isolation Kit is a two-step method designed to isolate CD27+ B cells from fresh or previously frozen peripheral blood mononuclear cells. First, CD27+ cells are isolated by column-free immunomagnetic positive selection using antibody complexes and EasySep™ Releasable RapidSpheres™. Then, bound magnetic particles are removed from the EasySep™-isolated CD27+ cells, and unwanted non-B cells are targeted for depletion using antibody complexes and EasySep™ Dextran RapidSpheres™. The final isolated fraction contains highly purified CD27+ memory B cells that are immediately ready for downstream applications. An optional protocol allows for the isolation of naïve B cells in parallel for use in functional studies.
![Research Products International Corp PMSF [Phenylmethylsulfonyl fluoride], 25 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-P20270-25.0.JPG-250.jpg)
Research Products International Corp PMSF [Phenylmethylsulfonyl fluoride], 25 Grams
Irreversible serine protease inhibitor of chymotrypsin, kallikrein, papain, subtilisin, thrombin and trypsin. Working Conentration: 0.1-1.0mM.
Bioassay Systems QuantiChrom™ Glutamate Dehydrogenase Assay Kit. For quantitative determination of glutamate dehydrogenase activity.
For quantitative determination of glutamate dehydrogenase activity. Key Features: Fast and sensitive. Linear detection range (20 µL sample): 0.4 to 80 U/L for 30 min reaction. Detection Limit of 0.1 U/L for 120 min reaction. Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: OD565nm. Samples: Serum, plasma, cell, tissue, agriculture etc. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 0.1 U/L.
Hampton Research HR2-900-01/StockOptions Tris #01 pH 7.0, 185 ml
HR2-900-01/StockOptions Tris #01 pH 7.0, 185 ml. Please note items from this supplier are non-returnable.
Chem-Impex International, Inc. Tween 20 | 9005-64-5 | MFCD00165986 | 1L
Tween 20, 9005-64-5, MFCD00165986, 1L
Chem-Impex International, Inc. Tween 20 | 9005-64-5 | MFCD00165986 | 100ML
Tween 20, 9005-64-5, MFCD00165986, 100ML
Selleck Chemical LLC MG-101
MG-101 (ALLN Calpain inhibitor-1 Ac-LLnL-CHO) is a cell-permeable and potent inhibitor of cysteine proteases including calpains and lysosomal cathepsins