Test
Use for coupling antibodies or other large proteins containing -NH2 groups to the Sepharose media. CNBr-Activated Sepharose™ Medium is a pre-activated media perfect for custom affinity applications.
Simplify enrichment of target proteins by immunoprecipitation or pull-down applications
Particle Size | 37 to 100 μm |
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Content And Storage | 4°C to 8°C |
Matrix | Paramagnetic spherical, highly cross linked agarose particles |
Rapid processing of large volumes, recoveries of 70 to 90%. Cytiva Protein G Sepharose™ 4 Fast Flow Media is recombinant protein G coupled to Sepharose 4 Fast Flow.
Particle Size | 90 μm |
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Content And Storage | 2°C to 8°C |
Matrix | 4% Cross Linked Agarose |
Ligand | Recombinant Protein G lacking albumin binding region |
Ligand Concentration | 2mg Protein G/mL Medium |
pH | 3 to 9 |
For Use With (Application) | Used in a range of diagnostic and research applications |
Used for coupling compounds containing carboxyl groups to Sepharose 4B. EAH Sepharose™ 4B Medium provides extremely stable coupling via an 11-atom spacer arm.
Routinely used in the preparation and purification of a number of glycoproteins and carbohydrate-containing molecules
NHS Mag Sepharose magnetic beads for convenient enrichment of selected proteins using pull-down sample preparation techniques
Particle Size | 37 to 100 μm |
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Content And Storage | 4°C to 8°C |
Matrix | Paramagnetic spherical, highly cross linked agarose particles |
Bead Type | NHS Mag Sepharose |
Ligand | N-hydroxysuccinimide |
Ligand Concentration | 8 to 14μM/mL |
Magnetic beads are designed for rapid, small-scale purification and screening of monoclonal and polyclonal antibodies from serum and cell supernatants
Binding Capacity | 27mg human IgG/mL gel |
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IgG Affinity Gel is prepared by reaction of purified IgG to activated Sepharose™ 4B gel.
For high resolution preparative gel filtration chromatography with short run times and good recovery. Cytiva Lifescience™ HiLoad™ Superdex™ 75 pg are prepacked XK columns.
For Use With (Application) | high resolution preparative gel filtration chromatography |
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Use for small-scale sample preparation using magnetic beads. Cytiva Life Sciences™ MagRack 6 enables handling of up to six samples in 1.5 ml microcentrifuge tubes.
Use for separating MAbs from dimers and contaminants. Cytiva Life Sciences™ HiLoad™ 26/600 Superdex™ 200 pg is ideal for high-resolution preparative gel filtration chromatography.
High binding capacity, 27mg human IgG/mL medium, for efficient capture of antibody with high yield and purity
Content And Storage | 4°C to 8°C |
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Matrix | Paramagnetic, spherical, highly cross-linked agarose particles |
Bead Type | Protein G Mag Sepharose Xtra magnetic bead |
Ligand | Protein G |
Binding Capacity | 27mg human IgG/mL gel |
For Use With (Application) | For rapid, small-scale purification and screening of monoclonal and polyclonal antibodies from serum and cell supernatants |
An affinity chromatography media for capture of glycosylated biomolecules including glycoproteins and glycolipids
Particle Size | 90 μm |
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Content And Storage | 2°C to 8°C |
Matrix | 4% Agarose |
Ligand | Concanavalin A |
Ligand Concentration | 13mg Con A/mL Drained Medium |
Binding Capacity | 30mg/mL |
pH | 4 to 9 |
For Use With (Application) | For separation and purification of Glycoproteins, Polysaccharides and Glycolipids, isolates cell surface Glycoproteins from detergent-solubilized membranes |
A pre-activated medium for immobilization of various ligands including sugars through coupling of hydroxy, amino or thiol groups on the ligand to Sepharose 6B via a 12-atom hydrophilic spacer arm
Use for purification of proteins with affinity for calmodulin, a highly conserved regulatory protein involved in many cellular processes in eukaryotic cells. Calmodulin Sepharose™ 4B Medium provides single-step purification.