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Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes This structure consists of approximately 146 bp of DNA wrapped around a nucleosome an octamer composed of pairs of each of the four core histones (H2A H2B H3 and H4) The chromatin fiber is further compacted through the interaction of a linker histone H1 with the DNA between the nucleosomes to form higher order chromatin structures This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family Transcripts from this gene lack polyA tails instead they contain a palindromic termination element This gene is found in the large histone gene cluster on chromosome 6p22-p21 3
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The ChromoTek GFP-Trap Magnetic Agarose are affinity beads for IP of GFP-fusion proteins. It comprises a GFP Nanobody/ VHH coupled to magnetic agarose beads.
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Biocolor Blyscan Glycosaminoglycan (sGAG) Dye Dissociation Reagents Component Pack (2 x 2 x 110 ml) Includes 2 x 110 ml Dye Reagent 2 x 110 ml Dissociation Reagent The Blyscan Glycosaminoglycan Assay is a quantitative colorimetric dye-binding method for the analysis of sulfated proteoglycans and glycosaminoglycans (sGAG) It can be used to measure the total sGAG content and can also be used to determine the O- and N- sGAG ratio within test samples The assay uses 1 9-dimethylmethylene blue dye label which is employed under conditions that provide a specific label for the sulfated polysaccharide component of proteoglycans or the protein free sGAG chains Applications Cell culture derived material soluble sGAGs from fluids (ex synovial fluid urine) fibrous and hyaline cartilages tissues (ex arteries heart lung skin tumors) Soluble sGAGs may be assayed directly solid materials will require a preliminary papain extraction Sensitivity 0 5 g Assay run time 1 hr
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