Protein Purification Reagents and Kits
Echelon Biosciences Research Labs PI(4)P PIP Beads 1 mL
PI(4)P PIP BeadsTM are composed of agarose with 10 nanomoles of bound PI(4)P per mL of beads, enough for several protein binding experiments. Each 1 mL order of PIP Beads includes a small amount of control beads. Larger quantities of Control Beads are also available for purchase. This item is non returnable
SIGMA ALDRICH FINE CHEMICALS BIOSCIENCES COMPLETE HIS-TAG PURIFI200ML
NC2186970 COMPLETE HIS-TAG PURIFI200ML
Enzo Life Sciences Puromycin. 2HCl (250mg). CAS: 58-58-2
Inhibits protein synthesis. Inhibits translation by causing premature release of nascent polypeptide. Induces apoptosis. Alternative name: 3'-[[2-Amino-3-(4-methoxyphenyl)-1-oxopropyl]amino]-3'-deoxy-N,N-dimethyl-adenosine . 2HCl, 3'-(alpha-Amino-p-methoxy-hydrocinnamamido-3'-deoxy-N,N-dimethyl-adenosine . 2HCl. Purity: ≥98% (HPLC, TLC). Solubility: Soluble in water (50mg/ml). Long Term Storage: -20°C.
Enzo Life Sciences Granzyme B assay kit for drug discovery (1x96wells)
Granzyme B is a serine protease found in the lymphocyte granules of cytotoxic T-lymphocytes (CTLs) and natural killer (NK) cells. Activated granzyme B prefers an aspartic acid residue at the P1 site of its substrates; a preference shared by only one other group of mammalian proteases, the caspases. Granzyme B is required for the rapid apoptotic signal delivered by CTLs and NK cells, an effect mediated by its ability to cleave and activate various procaspases. Granzyme B plays a crucial role in the development of acute graft-vs.-host disease. Thus, the inhibition of this enzyme may prove to be an important tool in controlling immune responses. Conversely, the failure of granzyme B-induced apoptosis may contribute to certain pathologies. Applications: Colorimetric detection, Fluorescent detection, HTS Activity assay.
Proteintech Group Inc V5-Trap Magnetic Particles M-270
The ChromoTek V5-Trap Magnetic Particles M-270 consists of an anti-V5-tag Nanobody (VHH), which is covalently bound to Magnetic Particles M-270. V5-Trap Magnetic Particles M-270 is used to immunoprecipitate V5-tagged fusion proteins from cell extracts of various organisms like mammals, plants, bacteria, yeast, insects etc.
Proteintech Group Inc MBP-Trap Agarose beads
The ChromoTek MBP-Trap Agarose are affinity beads for IP of MBP-fusion proteins. They comprise MBP Nanobodies/ VHHs coupled to agarose beads.
Proteintech Group Inc V5-Trap Agarose Kit
V5-Trap Agarose is an affinity reagent for IP and purification of V5-tagged proteins. It consists of an anti-V5 Nanobody/ VHH coupled to agarose beads.
Hampton Research HR2-930-B4/Grid Screen MPD #B4, 185 ml - 0.1 M HEPES pH 7.0, 20% (+/-)-2-Methyl-2,4-pentanediol
HR2-930-B4/Grid Screen MPD #B4, 185 ml - 0.1 M HEPES pH 7.0, 20% (+/-)-2-Methyl-2,4-pentanediol. Please note items from this supplier are non-returnable.
ILEX LIFE SCIENCES LLC EndoTrap HD 5/1, 5 mL resin column, Affinity chromatography endotoxin (LPS) removal kit
EndoTrap HD endotoxin removal products, manufactured in Germany by LIONEX, are intended for in vitro quantitative removal of lipopolysaccharide (LPS) from biological samples in aqueous solutions such as proteins, antibodies, cell extracts, phage, and nucleic acids. EndoTrap HD can be used in small scale processes for R&D and large scale manufacturing processes. It can be applied in early or late biomanufacturing process steps. EndoTrap HD is based on hydrophilic, dimensionally stable affinity matrix with excellent pressure/flow characteristics.Method: affinity chromatography.Format: pre-filled gravity flow columns, pre-filled FPLC columns, or bulk resin.LPS Binding Capacity: > 5,000,000 EU/mL.LPS Removal Rate: > 99.99%.Sample Recovery Rate: > 95%.pH Sample Range: 4-10.Non-toxic, no cytotoxic effects.Re-use more than 10 times.Suitable for high-salt conditions.The EndoTrap HD Leakage ELISA is available for quantitative detection of leached ligand from EndoTrap HD resin.
LifeSensors Tandem Ubiquitin Binding Entities (TUBE1) Agarose 1mL
Based on protein domains known to possess an affinity for ubiquitin, Tandem Ubiquitin Binding Entities (TUBEs) have been developed for the isolation and identification of ubiquitinated proteins. TUBEs display up to a 1000-fold increase in affinity for polyubiquitin moieties over the single ubiquitin binding associated domain (UBA). In addition, TUBEs protect polyubiquitinated proteins from deubiquitination and proteasomal degradation, allowing for detection at relatively low abundance. These properties effectively allow TUBEs to “capture” protein in its polyubiqutinated state. TUBEs can be used to isolate, enrich, and identify ubiquitinated proteins from cell and tissue extracts. Agarose coupled TUBEs allow for efficient recovery of polyubiquitinated proteins in a single step. Polyubiquitinated protein can easily be separated from TUBEs for proteomic studies or identification by Western blotting. This product is protected by one or more US or Foreign patents.
Cayman Chemical GlutathIon PeroxIdase asy 96
GPX catalyzes the reduction of hydroperoxides, including H2O2, using reduced glutathione and thereby functions to protect the cell from oxidative damage. Cayman’s Glutathione Peroxidase Assay measures GPX activity indirectly by a coupled reaction with GR. Oxidized GSSG, produced upon reduction of an organic hydroperoxide by GPX, is recycled to its reduced state by GR and NADPH. The oxidation of NADPH to NADP+ is accompanied by a decrease in absorbance at 340 nm. The rate of decrease in the absorbance at 340 nm is directly proportional to the GPX activity in the sample. Cayman’s GPX Assay can be used to measure all of the glutathione-dependent peroxidases in plasma, erythrocyte lysates, tissue homogenates, and cell lysates.