Protein Purification Reagents and Kits
Abcam Immunoprecipitation kit, 25 Tests
Abcam's Immunoprecipitation Kit (ab206996) can be used to perform immunoprecipitation (IP) and Co-IP for functional studies of immunoprecipitated proteins/complexes and SDS-PAGE or western blot analysis of immunoprecipitated proteins and complexes.
The product is subject to the following: Abcam Restricted Use Statement
ABclonal Technology MAPKAPK-2/MK2 Rabbit pAb
This gene encodes a member of the Ser/Thr protein kinase family. This kinase is regulated through direct phosphorylation by p38 MAP kinase. In conjunction with p38 MAP kinase, this kinase is known to be involved in many cellular processes including stress and inflammatory responses, nuclear export, gene expression regulation and cell proliferation. Heat shock protein HSP27 was shown to be one of the substrates of this kinase in vivo. Two transcript variants encoding two different isoforms have been found for this gene.
Active Motif ChIP-IT Express Enzymatic Shearing Kit (included in 53009)
The first step in successful chromatin immunoprecipitation is shearing the chromatin into 200-1000 bp fragments. This has traditionally been performed by subjecting the isolated chromatin to different pulses of sonication. Sonication is an effective method for shearing DNA, and is the most widely-used method. In some circumstances, however, it can be difficult to optimize due to complications arising from emulsification and overheating. Because of this, Active Motif has developed a robust and user-friendly method to shear chromatin by enzymatic treatment. The Enzymatic Shearing Kit uses a proprietary Enzymatic Shearing Cocktail that quickly shears DNA into 200-1000 bp fragments. Because enzymatic shearing is solely time and temperature dependent, the problems associated with sonication are eliminated and ChIP results are improved.
LGC GENOMICS LLC (Lucigen) MPC Protein Precipitation Solution, 500 mLA simple solution and method for the removal of proteins from cell lysates prior to nucleic acid purification. To be used with the MasterPure Kits
This reagent is designed for use with the MasterPure™ kit protocols only.
Cayman Chemical MK2 InhIbItr IV 10mg
A highly selective, non-ATP competitive inhibitor of p38/mitogen-activated protein kinase-activated protein kinase 2 (IC50 = 0.11 μM); inhibits TNF-α (IC50 = 4.4 μM) and IL-6 (IC50 = 5.2 μM) secretion from the human THP-1 acute monocytic leukemia cell line
Takara Bio Capturem™ IP & Co-IP Kit | 12 Purifications
The Capturem IP & Co-IP Kit is designed for the simple and rapid capture of protein-protein complexes from whole cell extracts. The kit contains specially designed Protein A spin columns, using our novel Capturem technology for efficient capture of antibody-bound target protein complexes. The included buffer set provides ease of use and rapid isolation of immunoprecipitated protein complexes, allowing faster downstream analysis. Each column can hold up to 850 µl of sample and requires a minimum elution volume of 20 µl.
ABclonal Technology PARP1 Rabbit pAb
This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes.
Cayman Chemical Methylated LysIn PolycLNal
Immunogen: Methylated KLH • Host: Rabbit • Species reactivity: (+) Species independent • Applications: ELISA, ICC, IF, IP, WB • Lysine can be methylated once, twice, or three times by lysine methyltransferases. The transfer of methyl groups from SAM to histones is catalyzed by histone methyltransferases.
Cayman Chemical NavenIFlex Cel MR RED 100RXN
A DUSP26 inhibitor (IC50 11.62 UM) induces IMR-32 neuroblastoma cell apoptosis (IC50 4.13 UM)
Abcam Mycophenolate mofetil, MPDH inhibitor, 250MG
MW 433.5 Da, Purity >98%. Selective, non-competitive and reversible IMPDH inhibitor (IC₅₀ = 3 μM). Immunosuppressant agent. Inhibits guanosine synthesis. Shows antiparasitic effects and suppresses T cells and B cells in vivo. Orally active.
The product is subject to the following: Abcam Restricted Use Statement
Abcam Cyclic Pifithrin-alpha hydrobromide, pifithrin-alpha analog, 50MG
MW 349.3 Da, Purity >98%. Stable, cell-permeable analog of pifithrin-α (ab120478). Inhibits p53-mediated apoptosis and p53-dependent gene transcription. Prevents dexamethasone-induced cell death in murine thymocytes (EC₅₀ = 2.01 μM). Potent STAT6 transcriptional inhibitor. Active in vivo and in vitro. See similar compound
The product is subject to the following: Abcam Restricted Use Statement
Proteintech Group Inc DYKDDDDK Fab-Trap™ Agarose
The ChromoTek DYKDDDDK Fab-Trap™ Agarose consists of an anti-DYKDDDDK-tag Fab-fragment, which is covalently bound to agarose beads. DYKDDDDK Fab-Trap™ Agarose is used to immunoprecipitate 1x and 3xDYKDDDDK-tagged fusion proteins from cell extracts of various organisms like mammals, plants, bacteria, yeast, insects etc.
Active Motif ChIP-IT High Sensitivity
The ChIP-IT High Sensitivity (HS) Kit is designed to give superior performance when performing ChIP using antibodies against low abundance transcription factors, antibodies with sub-optimal binding affinities or when starting with a limited number of cells. Higher signal and lower non-specific DNA pulldown is achieved through the incorporation of specialized protein G agarose beads and optimized fixation, binding and wash buffers. In addition, washes are performed via gravity filtration, which is faster, easier and more consistent than magnetic bead capture. The high-quality ChIP DNA obtained from the ChIP-IT High Sensitivity Kit is also ideal for ChIP-Seq; ChIP-IT HS has been validated for use in this application, even when using antibodies that target low abundance transcription factors.
Abcam Pifithrin-alpha-HBr, p53 Inhibitor, 10MG
MW 367.3 Da, Purity >95%. p53 Inhibitor. Specifically blocks tumor supressor p53 transcriptional activity and can supress heat shock and glucocorticoid signalling pathways. Additionally offers protection to cells from p53 mediated apoptosis. Active in vivo; protects mice from chemotherapy side effects associated with p53 induction. Also aryl hydrocarbon receptor (AHR) agonist.
The product is subject to the following: Abcam Restricted Use Statement
Medchemexpress LLC p53 Rat His 10ug
Recombinant full-length rat p53 protein (M1-D391), expressed in Escherichia coli and supplied lyophilized with an N-terminal 6xHis tag. The protein is formulated for stability and provided at >90% purity for use in biochemical assays, antibody validation, protein-protein interaction studies, and assay development. Store frozen and follow the datasheet for reconstitution and handling to preserve activity.
- Full-length sequence M1-D391, matching accession P10361.
- N-terminal 6xHis tag for purification and detection.
- Expressed in Escherichia coli for high-yield production.
- Lyophilized formulation with stabilizing excipients for storage stability.
- Purity greater than 90% as determined by reducing SDS-PAGE.
- Suitable for biochemical assays, antibody validation, and interaction studies.
- Recommended to aliquot and store at -20°C or -80°C to minimize freeze-thaw.