Nutragen is a 6 mg/ml, type I bovine atelocollagen solution for 2D and 3D cell culture. Due to having a higher concentration, Nutragen hydrogels will be stronger than other lower concentration collagen products. Nutragen collagen is known as the standard of all collagens for purity (>99% collagen content), functionality, and the most native-like collagen available. Nutragen is isolated from bovine hides sourced from the only controlled, closed herd in the United States. Nutragen collagen is approximately 97% Type I collagen with the remainder being comprised of Type III collagen. Nutragen collagen is supplied at approximately a 6 mg/mL concentration to provide an extremely firm gel. Nutragen is soluble atelo-collagen in 0.01 N HCI, therefore, the pH is approximately 2.0. Nutragen collagen is ideal for coating of surfaces, providing preparation of thin layers for culturing cells, or use as a solid gel. Nutragen collagen is provided in a 50 mL volume and is sterile filtered.