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A DNA sequence encoding the Influenza A virus (A/Wisconsin/588/2019 (H1N1)) hemagglutinin (EPI1661758) (Met1-Gly519, with a mutation Tyr108Phe), termed as HA, was expressed with a c-terminal AVI tagged polyhistidine tag at the C-terminus. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.
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