This kit detects EBI3 (Interleukin-27 subunit beta) in human serum plasma and tissue homogenates using a sandwich ELISA method It offers a quantitative detection range of 15 6 pg/mL to 1000 pg/mL with a sensitivity of 3 9 pg/mL ensuring precise measurement of low-abundance samples The assay requires a short incubation period of 1-5 hours and utilizes 50-100 L of sample per test Absorbance is measured at 450 nm for result quantification providing a reliable tool for researchers studying immune responses inflammatory diseases or EBI3-related pathways The kit is designed for flexibility across multiple biological matrices while maintaining high specificity and reproducibility in experimental settings