DNA Polymerases
Alkali Scientific Radiant™ HiFi Ultra Polymerase 1000 Reactions 5 x 100μL, 2u/μL HiFi Ultra Polymerase & 15 x 1mL 5x HiFi ultra Buffer (includes dNTP’s)
Ultra-High Fidelity Including dNTP's RadiantTM HiFi Ultra DNA Polymerase is a high-performance, high-fidelity DNA Polymerase optimized for the sensitive DNA amplification of a wide range of DNA templates including complex mammalian genomic DNA. RadiantTM HiFi Ultra Polymerase possesses 5´-3´ DNA polymerase and 3´-5´ proofreading exonuclease activities with an error-rate of 4.55 x 10-7 . The polymerase is ideal for cloning applications, library construction, screening and genotyping of problematic GC-rich and AT-rich sequences. RadiantTM HiFi Ultra Polymerase is engineered for robust, superior high-fidelity PCR in comparison with standard proof-reading enzymes such as pfu DNA Polymerase. Several point-mutations combined with a new-generation buffer formulation provide for significantly improved processivity for faster cycling, greater sensitivity and less inhibition with crude DNA samples. The supplied 5X HiFi Ultra Reaction Buffer contains an ideal concentration of MgCl2 and
Alkali Scientific Radiant™ Taq DNA Polymerase, 5u/µl, 10,000 units w/ separate tube 10X PCR buffer and MgCl2
Radiant™ Taq DNA Polymerase Description RadiantTM Taq DNA Polymerase is a highly purified, high performance DNA Polymerase optimized for the sensitive DNA amplification of a wide range of DNA templates including complex mammalian genomic DNA. RadiantTM Taq DNA Polymerase exhibits 5´-3´ DNA polymerase activity with an error-rate of wild-type Taq ( 2.0 x 10-5). The polymerase is ideal for screening, colony PCR, high-throughput PCR and genotyping of problematic GC-rich and AT-rich sequences. RadiantTM Taq DNA Polymerase is engineered for robust, superior PCR and is supplied with a highly optimized, new-generation buffer system which provides exceptional sensitivity. Storage RadiantTM Taq DNA Polymerase is shipped on blue or dry ice and should be stored at –20°C upon receipt. Excessive freeze/thawing should be avoided. When stored as specified, RadiantTM Taq DNA Polymerase is stable for 12 months from date of receipt. The Kit may also be stored at 4°C for 1 month. Important Cons
INTACT GENOMICS INC T4 DNA Polymerase 500 Units
IG™ T4 DNA Polymerase has both a DNA-dependent DNA polymerase activity and a potent 3´→5´ exonuclease activity. Applications 3´-overhang removal to form blunt ends (1, 2). 5´-overhang fill-in to form blunt ends (1, 2). Probe labeling using replacement synthesis (2). DNA library preparation for Next-generation sequencing. Ligation-independent cloning of PCR products. Second strand synthesis in site-directed mutagenesis (3).
INTACT GENOMICS INC i7 High-Fidelity DNA Polymerase 2X Master Mix 100 Reactions
Intact Genomics (IG) i7 High-Fidelity DNA Polymerase 2x master mix is ready to use premix which contains i7 high-fidelity DNA Polymerase, dNTPs, MgCl2, PCR enhancers and stabilizers with optimized proprietary reaction buffer. i7 high fidelity DNA Polymerase is a genetically engineered, heat stable DNA polymerase which has 5´→3´ polymerase and 3´→5´ exonuclease (proofreading) activities. This enzyme has high fidelity, sensitivity and processivity with an error rate ~2.8×102-fold lower than Taq DNA polymerase, and significantly lower than other proofreading enzymes in the marketplace (1).Applications Long and difficult template DNA amplification Cloning High-fidelity PCR Efficient for amplifying high GC content template DNA with magic enhancer.
As One International Inc Red-Taq DNA Polymerase 5 U/ul 10x Combination Buffer, Tween free, 10,000U
AS ONE Taq DNA Polymerase with red dye is a thermostable recombinant DNA polymerase, which exhibits very high activity in primer extension and other molecular biology applications. Taq contains a red dye which provides easy and quick identification of reactions to which enzyme was added and allows confirmation of complete mixing. The inert dye has no effect on downstream processes. Taq with Red Dye is added directly to the reaction mix and is used in the same manner as standard Taq DNA Polymerase. AS ONE Taq DNA Polymerase with red dye has both a 5' to 3' DNA polymerase and a 5' to 3' exonuclease activity. The enzyme lacks a 3' to 5' exonuclease activity. Taq DNA Polymerase leaves an A prime overhang, which makes the enzyme ideal for TA cloning. Red Taq Polymerase includes Tween free 10x Combination Buffer.
As One International Inc Hot Start Taq Polymerase 5U/ul, 10x Ammonium Buffer and 10x Combination Buffer both Magnesium free, 5,000U
AS ONE HS DNA Polymerase is a modified form of AS ONE Taq DNA Polymerase, which is activated by heat treatment. A chemical moiety is attached to the enzyme at the active site, which renders the enzyme inactive at room temperature. Thus, during setup and the first ramp of thermal cycling, the enzyme is not active and misprimed primers are not extended. The result is higher specificity and greater yields when compared to standard DNA polymerases. Once the reaction reaches optimal activating temperature, the chemical moiety is cleaved during a 15 minute heat activation step, releasing the active HS Polymerase DNA Polymerase into the reaction. HS Taq includes magnesium free 10x Ammonium Buffer and 10x Combination Buffer
As One International Inc Taq DNA Polymerase 5 U/ul, 10x Ammonium Buffer and 10x Combination buffer both Magnesium and detergent free , 500U
AS ONE's Taq DNA Polymerase is a thermostable recombinant DNA polymerase, which exhibits very high activity in primer extension and other molecular biology applications. The enzyme is isolated from Thermus aquaticus and has a molecular weight of approximately 94 kDa.Taq DNA Polymerase has both a 5' to 3' DNA polymerase and a 5' to 3' exonuclease activity. The enzyme lacks a 3' to 5' exonuclease activity (no proofreading ability). Taq DNA Polymerase leaves an A prime overhang, which makes the enzyme ideal for TA cloning. Taq Polymerase includes Tween/Triton/Magnesium free 10x Ammonium Buffer and 10x Combination Buffer.
As One International Inc Taq DNA Polymerase 2x Master Mix, 1.5 mM MgCl2 (final concentration), 5,000Rxn
AS ONE's Taq DNA Polymerase Pre-Mix is a ready-to-use 2.0X reaction mix. Simply add primers, template, and water to successfully carry out primer extensions and other molecular biology applications. AS ONE Taq DNA Polymerase, the NH4+ buffer system, dNTPs, and magnesium chloride are present in Taq DNA Polymerase Mix. Each reaction requires 25 uL of the 2.0X reaction mix. Simply add primers, template and water to a total reaction volume of 50 uL. Taq DNA Polymerase Pre-Mix offers several advantages. Set up time is significantly reduced. The chance of contaminating component stocks is eliminated. Reduction of reagent handling steps leads to better reproducibility. Standard tests can be set up with the confidence that results will be consistent every time.
As One International Inc Taq DNA Polymerase 5 U/ul, 10x Ammonium Buffer and 10x Combination buffer both Magnesium and detergent free , 1,000U
AS ONE's Taq DNA Polymerase is a thermostable recombinant DNA polymerase, which exhibits very high activity in primer extension and other molecular biology applications. The enzyme is isolated from Thermus aquaticus and has a molecular weight of approximately 94 kDa.Taq DNA Polymerase has both a 5' to 3' DNA polymerase and a 5' to 3' exonuclease activity. The enzyme lacks a 3' to 5' exonuclease activity (no proofreading ability). Taq DNA Polymerase leaves an A prime overhang, which makes the enzyme ideal for TA cloning. Taq Polymerase includes Tween/Triton/Magnesium free 10x Ammonium Buffer and 10x Combination Buffer.
As One International Inc Taq DNA Polymerase 5 U/ul, 10x Ammonium Buffer and 10x Combination buffer both Magnesium and detergent free , 5,000U
AS ONE's Taq DNA Polymerase is a thermostable recombinant DNA polymerase, which exhibits very high activity in primer extension and other molecular biology applications. The enzyme is isolated from Thermus aquaticus and has a molecular weight of approximately 94 kDa.Taq DNA Polymerase has both a 5' to 3' DNA polymerase and a 5' to 3' exonuclease activity. The enzyme lacks a 3' to 5' exonuclease activity (no proofreading ability). Taq DNA Polymerase leaves an A prime overhang, which makes the enzyme ideal for TA cloning. Taq Polymerase includes Tween/Triton/Magnesium free 10x Ammonium Buffer and 10x Combination Buffer.
As One International Inc Taq DNA Polymerase 5 U/ul, 10x Ammonium Buffer and 10x Combination Buffer both detergent free, 10,000U
AS ONE's Taq DNA Polymerase is a thermostable recombinant DNA polymerase, which exhibits very high activity in primer extension and other molecular biology applications. The enzyme is isolated from Thermus aquaticus and has a molecular weight of approximately 94 kDa.Taq DNA Polymerase has both a 5' to 3' DNA polymerase and a 5' to 3' exonuclease activity. The enzyme lacks a 3' to 5' exonuclease activity (no proofreading ability). Taq DNA Polymerase leaves an A prime overhang, which makes the enzyme ideal for TA cloning. Taq Polymerase includes Tween/Triton free 10x Ammonium Buffer and 10x Combination Buffer.
INTACT GENOMICS INC Taq DNA Polymerase 2x Premix 500 Reactions
IG™ Taq DNA Polymerase 2x Premix with Dye is a thermostable DNA polymerase that possesses a 5´→3´ polymerase activity (1, 2) and a 5´ flap endonuclease activity (3, 4). Ideal for primary extension reaction with DNA fragments having dA overhang on 3’ ends.IG™ Taq DNA Polymerase 2x Premix with Dye is ready to use, containing Taq DNA Polymerase, dNTPs, MgCl2, and stabilizers. It has been optimized for routine PCR applications.Applications PCR Primer extension Colony PCR
As One International Inc Taq DNA Polymerase 5 U/ul glycerol free, 10x Ammonium Buffer and 10x Combination Buffer both Magnesium free, 500U
Taq DNA Polymerase is a thermostable recombinant DNA polymerase, which exhibits very high activity in primer extension and other molecular biology applications. The enzyme is isolated from Thermus aquaticus and has a molecular weight of approximately 94 kDa. Taq DNA Polymerase has both a 5' to 3' DNA polymerase and a 5' to 3' exonuclease activity. The enzyme lacks a 3' to 5' exonuclease activity (no proofreading ability). Taq DNA Polymerase leaves an A prime overhang, which makes the enzyme ideal for TA cloning. Glycerol free Taq is ideal for automation and free drying applications. Included with Taq are magnesium free 10x Ammonium Buffer and 10x Combination Buffer.