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DNA polymerases are enzymes that synthesize DNA molecules from nucleoside triphosphates. Products include various types of DNA polymerases, kits, buffers, and other reagents for DNA amplification.
Taq DNA Polymerase is a highly processive 5-3 DNA polymerase that lacks 3-5 exonuclease activity. It is a single polypeptide chain with a molecular weight of approximately 95 kDa.Taq DNA Polymerase was originally isolated from the thermophilic eubacterium Thermus aquaticus BM a strain lacking Taq I restriction endonuclease. The enzyme preparation obtained from E.coli is free of nonspecific engo- or exonucleases according to the current quality control procedures.
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fastStart(TM) Taq DNA Polymerase is a versatile enzyme that can be used in a wide variety of applications and on multiple instrument platforms. This modified thermostable recombinant Taq DNA polymerase is inactive at temperatures below 75 C but is activated by a 2 to 4 minute heat activation step at 95 C. Since it is inactive at low temperatures fastStart(TM) Taq DNA Polymerase cannot elongate non-specific primer-template hybrids that may form at those temperatures. fastStart(TM) Taq DNA Polymerase is an ideal tool for hot start PCR because the enzyme remains inactive during PCR set-up and prior to the initial denaturation step.
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T7 RNA polymerase is commonly used to transcribe DNA which has been cloned into vectors which have two phage promoters in opposite orientation. RNA can be selectively synthesized from either strand of the insert DNA with different polymerases. Homogeneously labeled single-stranded RNA can be generated with this system. Transcripts can be non-radioactively labeled with biotin or DIG-11-UTP or radioactively labeled to high specific activity with [alpha-32P] or [alpha-35S]-labeled nucleotides.Synthesis of hybridization probes T7 RNA polymerase allows highly efficient production of homogeneously labeled RNA. This labeled RNA may be used as hybridization probes in Southern northern and dot blots as well as in situ hybridizations.Suitable labelsTranscripts can be nonradioactively labeled with biotin-16-UTP or DIG-11-UTP. They may also be radioactively labeled to high specific activity with [alpha-32P]- or [alpha-35S]-labeled nucleotides.
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The enzyme dNTPack comprises fastStart Taq DNA Polymerase and a ready-to-use SOLUTIONon of PCR grade nucleotides. fastStart Taq DNA Polymerase is a versatile enzyme that can be used in a wide variety of applications and on multiple instrument platforms. This modified thermostable recombinant Taq DNA polymerase is inactive at temperatures below 75 C but is activated by a 2 to 4 minute heat activation step at 95 C. Since it is inactive at low temperatures fastStart Taq DNA Polymerase cannot elongate non-specific primer-template hybrids that may form at those temperatures. An optimized PCR buffer system and a GC-RICH SOLUTIONon to enable the enzyme to handle a wide range of templates are also supplied.
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