Ready-to-use Master Mix, in the 2X format Red with Taq Plus DNA polymerase to which users only need to add template, primers and H2O for the reaction. The Taq Plus Master Mix, in the 2X format Red also contains an inert, non-toxic red dye to visualize mixing; which also allows direct loading on gels after PCR for electrophoresis. Taq Plus DNA Polymerase is a modified version of Taq DNA Polymerase, thus improving the fidelity and amplification length of the resulting DNA fragment. Amplified DNA contains both blunt-ended fragments and those with 3’-A overhangs, which allows users to choose either blunt-end or T-vector protocols to clone the amplified products.