DNA Polymerases
As One International Inc Taq DNA Polymerase 5 U/ul glycerol free 10x Combination Buffer, Magnesium free and Tween free, 10,000U
Taq DNA Polymerase 5 U/ul glycerol free 10x Combination Buffer, Mg2+ free and Tween free. Glycerol free is ideal for automation and free drying applications
New England Biolabs, Inc. Bst-XT WS GF - 8000 units
Bst-XT WarmStart DNA Polymerase (Glycerol-free) is formulated without glycerol to support lyophilization incorporation into microfluidic devices and enable quick adoption into automation workflows Bst-XT WarmStart DNA Polymerase is an engineered variant of Bacillus stearothermophilus DNA Polymerase Large Fragment fused to a novel nucleic acid binding domain for improved isothermal amplification performance and increased reverse transcription activity This enzyme combines the polymerization speed of Bst 3 0 and the high specificity of Bst 2 0 DNA polymerases Bst-XT WarmStart DNA Polymerase contains a reversibly bound aptamer which inhibits polymerase activity at room temperature The aptamer rapidly releases above 45 C therefore no separate activation step is needed Bst-XT WarmStart DNA Polymerase contains 5 to 3 DNA polymerase activity with either DNA or RNA templates and strong strand displacement activity but lacks 5 to 3 and 3 to 5 exonuclease activity
Sigma Aldrich Fine Chemicals Biosciences Taq DNA Polymerase 1 U/mul
The enzyme was cloned in E.coli and is isolated to be free of unspecific endo- or exonucleases according to the current quality control procedurese. Taq DNA Polymerase is a highly processive 5primendash3prime DNA polymerase that lacks 3primendash5prime exonuclease activity. It consists of a single polypeptide chain with a molecular weight of approximately 95 kDa. The enzyme exhibits highest activity at a pH of around 9 (adjusted at 20 C) and temperatures around 75 C. Taq DNA Polymerase also accepts modified deoxyribonucleosidetriphosphates as substrates and can be used to label DNA-fragments either with radionucleotides digoxigenin fluorescein or biotin.The high processivity absence of exonuclease activity and temperature optima of Taq DNA Polymerase enable the use of this enzyme in DNA sequencing especially where the reSOLUTIONon of secondary structures plays a major role.
New England Biolabs, Inc. Hot Start Taq DNA Polymerase – 1000 units
Hot Start Taq DNA Polymerase is a mixture of Taq DNA Polymerase and an aptamer-based inhibitor. The inhibitor binds reversibly to the enzyme, inhibiting polymerase activity at temperatures below 45C, but releases the enzyme during normal cycling conditions, allowing reactions to be set up at room temperature. This aptamer-based hot start does not require a separate high temperature incubation step to activate the enzyme. Taq DNA polymerase possesses a 5' to 3' polymerase activity and a 5' flap endonuclease activity. It is supplied with 10X Standard Taq Reaction Buffer, which is detergent-free and designed to be compatible with existing assay systems.
New England Biolabs, Inc. LongAmp Taq 2X Master Mix – 100 reactions (50 µl vol)
The LongAmp Taq 2X Master Mix combines high quality NEB recombinant Taq DNA Polymerase with a trace amount of Deep Vent DNA Polymerase. The 3' to 5' exonuclease activity of Deep Vent DNA Polymerase increases the fidelity and robust amplification of Taq DNA Polymerase. This ready-to-use mix offers two fold higher fidelity than Taq DNA Polymerase alone. A wide range of PCR products can be generated, up to 30 kb from lambda or human genomic DNA.
New England Biolabs, Inc. BaeGI – 500 units
A restriction endonuclease that recognizes the sequence G_KGCM^C.
Sigma Aldrich Fine Chemicals Biosciences Taq DNA Polymerase 5 U/mul
Taq DNA Polymerase is a highly processive 5-3 DNA polymerase that lacks 3-5 exonuclease activity. It is a single polypeptide chain with a molecular weight of approximately 95 kDa.Taq DNA Polymerase was originally isolated from the thermophilic eubacterium Thermus aquaticus BM a strain lacking Taq I restriction endonuclease. The enzyme was cloned in E.coli.
Enzo Life Sciences Klenow (3'-5' exo-) (20µl)
Klenow (3′-5′ exo-) is a mesophilic DNA polymerase deficient in both proofreading (3′-5′) and nick-translation (5′-3′) nuclease activities, and that displays a moderate strand displacement activity during DNA synthesis. The protein is expressed as a truncated product of the E.coli PolA gene and contains the D355A and E357A mutations. Klenow is a mesophilic DNA polymerase deficient in both proofreading (3′-5′) and nick-translation (5′-3′) nuclease activities, and that displays a moderate strand displacement activity during DNA synthesis. Alternative name: Klenow fragment. Concentration: Unit Concentration: 50 U/µl Protein Concentration: 5 mg/ml. Purity: ≥99% (SDS-PAGE). Formulation: Liquid. In 20mM TRIS-HCl, pH 7.5, containing 50% glycerol, 0.1mM EDTA and 1mM DTT. Source: Recombinant E. coli. carrying the Klenow gene. Specificity: SS Exonuclease: 500U <0.1% released DS Exonuclease: 500U <0.1% released Endonuclease: 500U <0.1% converted E. coli 16S rDNA Contamination 500U <10 copies.
Alkali Scientific Radiant™ Hot Start Taq DNA Polymerase for FAM & SYBR green dye users constructing qPCR mix, 1000 Reactions, (4 x .05mL Hot-Start Taq Polymerase, 4 x 1.5mL 20X KCI Reaction Buffer)
Radiant™ Hot Start Taq DNA Polymerase for FAM & SYBR green dye users constructing qPCR mix Radiant™ Hot-Start Taq DNA Polymerase uses the latest developments in polymerase technology and buffer chemistry to enhance PCR speed, yield and specificity. Radiant™ Hot-Start Taq DNA Polymerase is especially suited to application where absence of residual host bacterial DNA is critical. Our HS Taq has an error rate of approximately 1 error per 2.0 x 105 nucleotides incorporated. PCR products generated with Radiant™ Hot-Start Taq Polymerase are A-tailed and may be cloned into TA cloning vectors.
Sigma Aldrich Fine Chemicals Biosciences Taq DNA Polymerase 5 U/mul
Taq DNA Polymerase is a highly processive 5-3 DNA polymerase that lacks 3-5 exonuclease activity. It is a single polypeptide chain with a molecular weight of approximately 95 kDa.Taq DNA Polymerase was originally isolated from the thermophilic eubacterium Thermus aquaticus BM a strain lacking Taq I restriction endonuclease. The enzyme was cloned in E.coli.
New England Biolabs, Inc. Taq DNA Polymerase with Standard Taq Buffer – 2000 units
Taq DNA Polymerase is a thermostable DNA polymerase that possesses a 5' to 3' polymerase activity and a 5' flap endonuclease activity. It is supplied with 10X Standard Taq Reaction Buffer, which is detergent-free and designed to be compatible with existing assay systems.