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Filtered Search Results
Thermo Scientific™ FastDigest RruI
Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer.
Thermo Scientific™ BspTI (AflII) (10 U/μL)
Cut at C^TTAAG sites with BspTI (AflII) restriction enzyme, which performs best at 37°C in O buffer (Isoschizomers: AflII, BfrI, Bst98I, BstAFI, MspCI, Vha464I).
Thermo Scientific™ BauI (BssSI) (10 U/μL)
Cut at CACGAG(-5/-1)^ sites with BauI (BssSI) restriction enzyme, which performs best at 37°C in Tango buffer (Isoschizomers: BssSI, Bst2BI).
Thermo Scientific™ MlsI (MscI) (5 U/μL)
The MlsI (MscI) restriction enzyme recognizes TGG^CCA sites and cuts best at 37°C in R buffer (Isoschizomers: BalI, MluNI, MscI, Msp20I).
| Sensitive to Heat Inactivation | Yes |
|---|---|
| Product Type | Restriction Enzyme |
| Enzyme | MlsI (MscI) |
| Methylation Sensitivity | Dcm Methylation-Sensitive,Not Dam Methylation-Sensitive,Not CpG Methylation-Sensitive |
| Research Category | Traditional Cloning |
| Compatible Buffer | 10X Buffer R |
| Type IIS RE | No |
| Optimal Reaction Temperature | 37°C |
| Concentration | 5 U/μL |
Thermo Scientific™ EcoRII (10 U/μL)
Cut at ^CCWGG sites with EcoRII restriction enzyme, which performs best at 37°C in O buffer (Isoschizomers: AjnI, BseBI, Bst2UI, BstNI, BstOI, Psp6I, PspGI).
Thermo Scientific™ PvuI (10 U/μL)
The PvuI restriction enzyme recognizes CGAT^CG sites and cuts best at 37°C in R buffer (Isoschizomers: BpvUI, MvrI, Ple19I).
| Sensitive to Heat Inactivation | Yes |
|---|---|
| Product Type | Restriction Enzyme |
| Enzyme | Pvu I |
| Methylation Sensitivity | CpG Methylation-Sensitive,Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive |
| Research Category | Traditional Cloning |
| Compatible Buffer | 10X Buffer R |
| Type IIS RE | No |
| Optimal Reaction Temperature | 37°C |
| Concentration | 10 U/μL |
Thermo Scientific™ SspDI (KasI) (10 U/μL)
Cut at G^GCGCC sites with SspDI (KasI) restriction enzyme, which functions best at 37°C in Tango buffer (Isoschizomers: BbeI, DinI, EgeI, KasI, Mly113I, NarI, SfoI).
Thermo Scientific™ FastDigest EheI
Cut at GGC^GCC sites with Thermo Scientific™ FastDigest™ EheI, which performs best using universal FastDigest Buffer (Isoschizomers: BbeI, DinI, EgeI, KasI, Mly113I, NarI, SfoI).
| Sensitive to Heat Inactivation | Yes |
|---|---|
| Product Type | Restriction Enzyme |
| Enzyme | EheI |
| Methylation Sensitivity | CpG Methylation-Sensitive,Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive |
| Research Category | Traditional Cloning |
| Compatible Buffer | 10X FastDigest Buffer/FastDigest Green Buffer |
| Overhang | Blunt |
| Type IIS RE | No |
| Optimal Reaction Temperature | 37°C |
| Inactivation Temperature | 65°C |
| Product Line | FastDigest |
Thermo Scientific™ FastDigest ScaI
Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer.
Thermo Scientific™ FastDigest VspI
Cut at AT^TAAT sites with Thermo Scientific™ FastDigest™ AseI (VspI), which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: PshBI).
Thermo Scientific™ Hin1II (NlaIII) (5 U/μL)
Cut at CATG^ sites with Hin1II (NlaIII) restriction enzyme, which performs best at 37°C in G buffer (Isoschizomers: CviAII, FaeI, FatI, Hsp92II, NlaIII).
Thermo Scientific™ RsaI (10 U/μL)
The RsaI restriction enzyme recognizes GT^AC sites and cuts best at 37°C in Tango buffer (Isoschizomers: AfaI, CviQI, RsaNI).
| Sensitive to Heat Inactivation | Yes |
|---|---|
| Product Type | Restriction Enzyme |
| Enzyme | Rsa I |
| Methylation Sensitivity | CpG Methylation-Sensitive,Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive |
| Research Category | Traditional Cloning |
| Compatible Buffer | 10X Buffer Tango |
| Type IIS RE | No |
| Optimal Reaction Temperature | 37°C |
| Concentration | 10 U/μL |
Thermo Scientific™ FastDigest PsyI
Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer.
Thermo Scientific™ AluI (10 U/μL)
Cut at AG^CT sites with AluI restriction enzyme, which performs best at 37°C in Tango buffer (Isoschizomers: AluBI).
| Sensitive to Heat Inactivation | Yes |
|---|---|
| Product Type | Restriction Enzyme |
| Enzyme | Alu I |
| Methylation Sensitivity | Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive |
| Research Category | Traditional Cloning |
| Compatible Buffer | 10X Buffer Tango |
| Type IIS RE | No |
| Optimal Reaction Temperature | 37°C |
| Concentration | 10 U/μL |
Thermo Scientific™ FastDigest Eco52I
Cut at C^GGCCG sites with Thermo Scientific™ FastDigest™ EagI (Eco52I), which performs best universal FastDigest Buffer (Isoschizomers: BseX3I, BstZI, EclXI).