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Filtered Search Results
Thermo Scientific™ FastDigest MssI
Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer.
Thermo Scientific™ Eam1105I (AhdI) (10 U/μL)
Cut at GACNNN^NNGTC sites with Eam1105I (AhdI) restriction enzyme, which performs best at 37°C in its own unique buffer (Isoschizomers: AhdI, AspEI, BmeRI, DriI, EclHKI).
Thermo Scientific™ FastDigest Eco52I
Cut at C^GGCCG sites with Thermo Scientific™ FastDigest™ EagI (Eco52I), which performs best universal FastDigest Buffer (Isoschizomers: BseX3I, BstZI, EclXI).
Thermo Scientific™ AluI (10 U/μL)
Cut at AG^CT sites with AluI restriction enzyme, which performs best at 37°C in Tango buffer (Isoschizomers: AluBI).
| Sensitive to Heat Inactivation | Yes |
|---|---|
| Product Type | Restriction Enzyme |
| Enzyme | Alu I |
| Methylation Sensitivity | Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive |
| Research Category | Traditional Cloning |
| Compatible Buffer | 10X Buffer Tango |
| Type IIS RE | No |
| Optimal Reaction Temperature | 37°C |
| Concentration | 10 U/μL |
Thermo Scientific™ ScaI (10 U/μL)
The ScaI restriction enzyme recognizes AGT^ACT sites and cuts best at 37°C in its own unique buffer (Isoschizomers: AssI, BmcAI, ZrmI).
| Sensitive to Heat Inactivation | Yes |
|---|---|
| Product Type | Restriction Enzyme |
| Enzyme | Sca I |
| Methylation Sensitivity | Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive |
| Research Category | Traditional Cloning |
| Compatible Buffer | Unique Buffer (10X Buffer ScaI) |
| Type IIS RE | No |
| Optimal Reaction Temperature | 37°C |
| Concentration | 10 U/μL |
Thermo Scientific™ FastDigest BglI
Cut at GCCNNNN^NGGC sites with Thermo Scientific™ FastDigest™ BglI, which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer.
Thermo Scientific™ SspDI (KasI) (10 U/μL)
Cut at G^GCGCC sites with SspDI (KasI) restriction enzyme, which functions best at 37°C in Tango buffer (Isoschizomers: BbeI, DinI, EgeI, KasI, Mly113I, NarI, SfoI).
Thermo Scientific™ PasI (10 U/μL)
Cut at CC^CWGGG sites with PasI restriction enzyme, which performs best at 55°C in its own unique buffer.
Thermo Scientific™ Buffer Set for Restriction Enzymes
Ensure the optimum reaction conditions for each restriction enzyme with this five buffer system.
Thermo Scientific™ BglI (10 U/μL)
Cut at GCCNNNN^NGGC sites with BglI restriction enzyme, which performs best at 37°C in O buffer.
| Sensitive to Heat Inactivation | Yes |
|---|---|
| Product Type | Restriction Enzyme |
| Enzyme | Bgl I |
| Methylation Sensitivity | CpG Methylation-Sensitive,Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive |
| Research Category | Traditional Cloning |
| Compatible Buffer | 10X Buffer O |
| Type IIS RE | No |
| Optimal Reaction Temperature | 37°C |
| Concentration | 10 U/μL |
Thermo Scientific™ NcoI (10 U/μL)
The NcoI restriction enzyme recognizes C^CATGG sites and cuts best at 37°C in Tango buffer (Isoschizomers: Bsp19I).
| Concentration | 10 U/μL |
|---|
Thermo Scientific™ Eco105I (SnaBI) (10 U/μL)
The Eco105I (SnaBI) restriction enzyme recognizes TAC^GTA sites and cuts best at 37°C in Tango buffer (Isoschizomers: BstSNI, SnaBI).
| Sensitive to Heat Inactivation | Yes |
|---|---|
| Product Type | Restriction Enzyme |
| Enzyme | Eco105I (SnaBI) |
| Methylation Sensitivity | CpG Methylation-Sensitive,Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive |
| Research Category | Traditional Cloning |
| Compatible Buffer | 10X Buffer Tango |
| Type IIS RE | No |
| Optimal Reaction Temperature | 37°C |
| Concentration | 10 U/μL |
Thermo Scientific™ TaqI (10 U/μL)
The TaqI restriction enzyme recognizes T^CGA sites and cuts best at 65°C in its own unique buffer.
| Concentration | 10 U/μL |
|---|
Thermo Scientific™ Bsh1236I (BstUI) (10 U/μL)
Cut at CG^CG sites with Bsh1236I (BstUI) restriction enzyme, which performs best at 37°C in R buffer (Isoschizomers: AccII, BspFNI, BstFNI, BstUI, MvnI).
| Sensitive to Heat Inactivation | Yes |
|---|---|
| Product Type | Restriction Enzyme |
| Enzyme | Bsh1236I (BstUI) |
| Methylation Sensitivity | CpG Methylation-Sensitive,Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive |
| Research Category | Traditional Cloning |
| Compatible Buffer | 10X Buffer R |
| Type IIS RE | No |
| Optimal Reaction Temperature | 37°C |
| Concentration | 10 U/μL |
Thermo Scientific™ Buffer R (10X)
Ensure the optimum reaction conditions for restriction enzymes with Thermo Scientific™ 10X Buffer R, premixed with BSA for enhanced stability.