Thermo Scientific™ DraI (10 U/μL)

Description

Thermo Scientific™ DraI is available as a FastDigest™ enzyme which is used for rapid DNA digestion.

Thermo Scientific™ DraI is available as a FastDigest™ enzyme which is used for rapid DNA digestion.

5'...T T T▵A A A...3'

3'...A A A▵T T T...5'

Conditions for 100% Activity:

• 1X Buffer Tango™:
• 33mM Tris-acetate (pH7.9 at 37°C), 10mM Mg-acetate, 66mM K-acetate and 0.1mg/mL BSA
• Incubate at 37°C

Storage Buffer:

• DraI is supplied in:10mM Tris-HCl (pH 7.5 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.15% Triton X-100, 0.2mg/mL BSA and 50% (v/v) glycerol

Ligation and Recleavage:

• After 50-fold overdigestion with DraI, more than 95% of the DNA fragments can be ligated and recut

Methylation Effects:

• Dam: never overlaps — no effect
• Dcm: never overlaps — no effect
• CpG: never overlaps — no effect
• EcoKI: may overlap — blocked
• EcoBI: never overlaps — no effect

Digestion of Agarose-embedded DNA:

• Minimum 5units of the enzyme are required for complete digestion of 1μg of agarose-embedded lambda DNA in 16hours

Specifications

• Concentration: 10 U/μL
• Methylation Sensitivity: Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive, Not CpG Methylation-Sensitive
• Enzyme: Dra I
• Compatible Buffer: 10X Buffer Tango
• Sensitive to Heat Inactivation: Yes
• Optimal Reaction Temperature: 37°C
• Type IIS RE: No
• Quantity: 1,500 units
• Product Type: Restriction Enzyme
• Research Category: Traditional Cloning

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.