Restriction Enzymes
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Filtered Search Results
Thermo Scientific™ BseJI (BsaBI) (10 U/μL)
Cut at GATNN^NNATC sites with BseJI (BsaBI) restriction enzyme, which performs best at 65°C in O buffer (Isoschizomers: BsaBI, Bse8I).
Thermo Scientific™ BoxI (PshAI) (10 U/μL)
Cut at GACNN^NNGTC sites with BoxI (PshAI) restriction enzyme, which performs best at 37°C in Tango buffer (Isoschizomers: BstPAI, PshAI).
Thermo Scientific™ FastDigest Eco57I
Cut at CTGAAG(16/14)^ sites with Thermo Scientifc™ FastDigest™ AcuI (Eco57I), which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer.
Thermo Scientific™ FastDigest SatI
Cut at GC^NGC sites with Thermo Scientific™ FastDigest™ Fnu4HI (SatI), which performs best using universal FastDigest Buffer (Isoschizomers: Fnu4HI, Fsp4HI, ItaI).
Thermo Scientific™ FastDigest XapI
FastDigest XapI restriction enzyme recognizes R^AATTY sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: AcsI, ApoI).
| Sensitive to Heat Inactivation | Yes |
|---|---|
| Product Type | Restriction Enzyme |
| Enzyme | XapI |
| Methylation Sensitivity | Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive |
| Research Category | Traditional Cloning |
| Compatible Buffer | 10X FastDigest Buffer/FastDigest Green Buffer |
| Overhang | 5' protruding ends |
| Type IIS RE | No |
| Optimal Reaction Temperature | 37°C |
| Inactivation Temperature | 80°C |
| Product Line | FastDigest |
Thermo Scientific™ FastDigest FokI
Cut at GGATG(N)9^ sites with Thermo Scientific™ FastDigest™ FokI, which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer.
Thermo Scientific™ BamHI, HC (50 U/μL)
The BamHI restriction enzyme recognizes G^GATCC sites and cuts best at 37°C in its own unique buffer.
Thermo Scientific™ BseMI (BsrDI) (5 U/μL)
Cut at GCAATG(2/0)^ sites with BseMI (BsrDI) restriction enzyme, which performs best at 55°C in R buffer (Isoschizomers: Bse3DI, BsrDI).
| Sensitive to Heat Inactivation | Yes |
|---|---|
| Product Type | Restriction Enzyme |
| Enzyme | BseMI (BsrDI) |
| Methylation Sensitivity | Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive |
| Research Category | Scarless Cloning |
| Compatible Buffer | 10X Buffer R |
| Type IIS RE | Yes |
| Optimal Reaction Temperature | 55°C |
| Concentration | 5 U/μL |
Thermo Scientific™ Bsp143I (Sau3AI) (10 U/μL)
Cut at ^GATC sites with Bsp143I (Sau3AI), which performs best at 37°C in its own unique buffer (Isoschizomers: BfuCI, BssMI, BstKTI, BstMBI, DpnII, Kzo9I, NdeII, Sau3AIm).
| Sensitive to Heat Inactivation | Yes |
|---|---|
| Product Type | Restriction Enzyme |
| Enzyme | Bsp143I (Sau3AI) |
| Methylation Sensitivity | CpG Methylation-Sensitive,Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive |
| Research Category | Traditional Cloning |
| Compatible Buffer | Unique Buffer (10X Buffer Bsp143I (Sau3AI)) |
| Type IIS RE | No |
| Optimal Reaction Temperature | 37°C |
| Concentration | 10 U/μL |
Thermo Scientific™ FastDigest XagI
Cut at CCTNN^NNNAGG sites with Thermo Scientific™ FastDigest™ EcoNI (XagI), which performs best using universal FastDigest Buffer (Isoschizomers: BstENI).
Thermo Scientific™ FastDigest TaiI
Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer.
Thermo Scientific™ FastDigest BveI
Cut at ACCTGC(4/8)^ sites with Thermo Scientific™ FastDigest™ BspMI (BveI), which performs best using universal FastDigest Buffer (Isoschizomers: Acc36I, BfuAI).
Thermo Scientific™ FastDigest BseLI
Cut at CCNNNNN^NNGG sites with Thermo Scientific™ FastDigest™ BslI (BseLI), which performs best using universal FastDigest Buffer (Isoschizomers: AfiI, Bsc4I).
Thermo Scientific™ FastDigest BoxI
Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer.
Thermo Scientific™ FastDigest FspBI
Cut at C^TAG sites with Thermo Scientific™ FastDigest™ BfaI (FspBI), which performs best using universal FastDigest Buffer (Isoschizomers: BfaI, MaeI, XspI).