Restriction Enzymes
Thermo Scientific™ BspLI (NlaIV) (10 U/μL)
Cut at GGN^NCC sites with BspLI (NlaIV) restriction enzyme, which performs best at 37°C in Tango buffer (Isoschizomers: BmiI, NlaIV, PspN4I).
Thermo Scientific™ FastDigest Alw26I (IIs class)
Cut at GTCTC(1/5)^ sites with Thermo Scientific™ FastDigest™ Alw26I, which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer.
Thermo Scientific™ SmaI, HC (50 U/μL)
Cut at CCC^GGG sites with SmaI restriction enzyme, which performs best at 30°C in Tango buffer (Isoschizomers: TspMI, XmaCI, XmaI).
Thermo Scientific™ BseNI (BsrI) (10 U/μL)
Cut at ACTGG(1/-1)^ sites with BseNI (BsrI) restriction enzyme, which performs best at 65°C in B buffer (Isoschizomers: Bse1I, BsrI, BsrSI).
Thermo Scientific™ Ecl136II, PacI, SacI Buffer (10X)
Optimize digestions with Ecl136II, PacI, SacI restriction enzymes using this buffer, premixed with BSA for enhanced stability.
Thermo Scientific™ FastDigest XagI
Cut at CCTNN^NNNAGG sites with Thermo Scientific™ FastDigest™ EcoNI (XagI), which performs best using universal FastDigest Buffer (Isoschizomers: BstENI).
Thermo Scientific™ Bsp143I Buffer (10X)
Optimize digestions with Bsp143I restriction enzyme using this buffer, premixed with BSA for enhanced stability.
Thermo Scientific™ CaiI (AlwNI) (10 U/μL)
Cut at CAGNNN^CTG sites with CaiI (AlwNI) restriction enzyme, which performs best at 37°C in Tango buffer (Isoschizomers: AlwNI).
Thermo Scientific™ Mph1103I (NsiI) (10 U/μL)
The Mph1103I (NsiI) restriction enzyme recognizes ATGCA^T sites and cuts best at 37°C in R buffer (Isoschizomers: EcoT22I, NsiI, Zsp2I).
Thermo Scientific™ FastDigest Cfr10I
Cut at R^CCGGY sites with Thermo Scientific™ FastDigest™ BsrFI (Cfr10I), which performs best using universal FastDigest Buffer (Isoschizomers: Bse118I, BssAI).
Invitrogen™ Anza™ 96 XmiI
Simplify traditional restriction enzyme cloning with the Invitrogen™ Anza™ Restriction Enzyme Cloning System.
Thermo Scientific™ BveI (BspMI) (5 U/μL)
Cut at ACCTGC(4/8)^ sites with BveI (BspMI) restriction enzyme, which performs best at 37°C in O (+oligo) buffer (Isoschizomers: Acc36I, BfuAI, BspMI).
Thermo Scientific™ FastDigest HpyF10VI
Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer.
Invitrogen™ Anza™ 6 NheI
Simplify traditional restriction enzyme cloning with the Invitrogen™ Anza™ Restriction Enzyme Cloning System.
Thermo Scientific™ BspOI (BmtI) (10 U/μL)
Cut at GCTAG^C sites with BspOI (BmtI) restriction enzyme, which performs best at 37°C in O buffer (Isoschizomers: AsuNHI, BmtI).