
Restriction Enzymes
- (1)
- (65)
- (597)
- (2)
- (1)
- (2)
- (1)
- (1)
- (4)
- (2)
- (2)
- (1)
- (1)
- (1)
- (2)
- (1)
- (4)
- (2)
- (1)
- (2)
- (2)
- (4)
- (9)
- (1)
- (5)
- (2)
- (1)
- (3)
- (2)
- (1)
- (2)
- (1)
- (1)
- (4)
- (5)
- (1)
- (2)
- (1)
- (1)
- (1)
- (2)
- (1)
- (2)
- (3)
- (2)
- (1)
- (2)
- (2)
- (2)
- (1)
- (2)
- (1)
- (1)
- (1)
- (1)
- (1)
- (2)
- (1)
- (1)
- (1)
- (2)
- (1)
- (1)
- (1)
- (2)
- (1)
- (2)
- (1)
- (1)
- (2)
- (1)
- (3)
- (2)
- (2)
- (1)
- (1)
- (1)
- (3)
- (2)
- (1)
- (2)
- (1)
- (2)
- (2)
- (1)
- (1)
- (2)
- (2)
- (1)
- (1)
- (1)
- (3)
- (3)
- (3)
- (2)
- (1)
- (1)
- (1)
- (1)
- (1)
- (1)
- (1)
- (1)
- (1)
- (3)
- (2)
- (1)
- (2)
- (1)
- (1)
- (1)
- (2)
- (1)
- (7)
- (4)
- (2)
- (2)
- (1)
- (1)
- (1)
- (1)
- (2)
- (2)
- (1)
- (1)
- (2)
- (2)
- (1)
- (3)
- (2)
- (3)
- (4)
- (2)
- (2)
- (2)
- (2)
- (1)
- (2)
- (1)
- (2)
- (1)
- (1)
- (2)
- (2)
- (1)
- (1)
- (1)
- (2)
- (1)
- (1)
- (8)
- (1)
- (1)
- (3)
- (1)
- (3)
- (2)
- (1)
- (1)
- (1)
- (3)
- (1)
- (2)
- (1)
- (2)
- (3)
- (2)
- (1)
- (1)
- (1)
- (1)
- (1)
- (1)
- (4)
- (9)
- (1)
- (1)
- (5)
- (3)
- (1)
- (2)
- (2)
- (2)
- (1)
- (2)
- (1)
- (2)
- (1)
- (2)
- (6)
- (2)
- (2)
- (2)
- (2)
- (2)
- (2)
- (1)
- (1)
- (2)
- (2)
- (2)
- (1)
- (4)
- (4)
- (2)
- (2)
- (4)
- (3)
- (2)
- (2)
- (1)
- (1)
- (2)
- (2)
- (3)
- (2)
- (3)
- (2)
- (1)
- (2)
- (1)
- (1)
- (1)
- (1)
- (7)
- (8)
- (7)
- (1)
- (1)
- (11)
- (1)
- (1)
- (1)
- (1)
- (2)
- (1)
- (3)
- (2)
- (2)
- (1)
- (2)
- (1)
- (2)
- (1)
- (2)
- (1)
- (2)
- (1)
- (1)
- (2)
- (1)
- (2)
- (1)
- (1)
- (2)
- (1)
- (2)
- (1)
- (1)
- (2)
- (6)
- (1)
- (1)
- (2)
- (1)
- (4)
- (5)
- (2)
- (4)
- (2)
- (2)
- (2)
- (5)
- (6)
- (2)
- (1)
- (2)
- (4)
- (1)
- (1)
- (3)
- (1)
- (1)
- (2)
- (1)
- (2)
- (1)
- (1)
- (3)
- (2)
- (7)
- (1)
- (1)
- (1)
- (1)
- (1)
- (3)
- (1)
- (1)
- (2)
- (1)
- (2)
- (3)
- (1)
- (2)
- (2)
- (3)
- (1)
- (3)
- (1)
- (1)
- (2)
- (2)
- (2)
- (2)
- (1)
- (1)
- (2)
- (1)
- (8)
- (1)
- (2)
- (9)
- (2)
- (2)
- (2)
- (2)
- (562)
- (81)
- (10)
- (574)
- (2)
- (24)
- (28)
- (90)
- (548)
Filtered Search Results

Thermo Scientific™ FastDigest BamHI

Cut at G^GATCC sites with Thermo Scientific™ FastDigest™ BamHI restriction enzyme, which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer.

Sensitive to Heat Inactivation | Yes |
---|---|
Product Type | Restriction Enzyme |
Enzyme | BamH I |
Methylation Sensitivity | Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive |
Research Category | Traditional Cloning |
Compatible Buffer | 10X FastDigest Buffer/FastDigest Green Buffer |
Overhang | 5' protruding ends |
Type IIS RE | No |
Optimal Reaction Temperature | 37°C |
Inactivation Temperature | 80°C |
Product Line | FastDigest™ |
Thermo Scientific™ FastDigest NotI

The FastDigest NotI restriction enzyme recognizes GC^GGCCGC sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: CciNI).
Sensitive to Heat Inactivation | Yes |
---|---|
Product Type | Restriction Enzyme |
Enzyme | Not I |
Methylation Sensitivity | CpG Methylation-Sensitive,Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive |
Research Category | Traditional Cloning |
Compatible Buffer | 10X FastDigest Buffer/FastDigest Green Buffer |
Overhang | 5' protruding ends |
Type IIS RE | No |
Optimal Reaction Temperature | 37°C |
Inactivation Temperature | 80°C |
Product Line | FastDigest™ |
Thermo Scientific™ FastDigest NcoI

The FastDigest NcoI restriction enzyme recognizes C^CATGG sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: Bsp19I).
Sensitive to Heat Inactivation | Yes |
---|---|
Product Type | Restriction Enzyme |
Enzyme | Nco I |
Methylation Sensitivity | Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive |
Research Category | Traditional Cloning |
Compatible Buffer | 10X FastDigest Buffer/FastDigest Green Buffer |
Overhang | 5' protruding ends |
Type IIS RE | No |
Optimal Reaction Temperature | 37°C |
Inactivation Temperature | 65°C |
Product Line | FastDigest™ |
Thermo Scientific™ FastDigest Bsu15I

Cut at AT^CGAT sites with ClaI (Bsu15I) restriction enzyme, which performs best at 37°C in 5-15 minutes using universal FastDigest™ Buffer.

Non-distribution item offered as a customer accommodation; additional freight charges may apply.
Learn More
Sensitive to Heat Inactivation | Yes |
---|---|
Product Type | Restriction Enzyme |
Enzyme | Bsu15I |
Methylation Sensitivity | CpG Methylation-Sensitive,Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive |
Research Category | Traditional Cloning |
Compatible Buffer | 10X FastDigest Buffer/FastDigest Green Buffer |
Overhang | 5' protruding ends |
Type IIS RE | No |
Optimal Reaction Temperature | 37°C |
Inactivation Temperature | 65°C |
Product Line | FastDigest™ |
Thermo Scientific™ FastDigest SmiI

Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer.

Thermo Scientific™ SgsI (AscI) (10 U/μL)
The SgsI (AscI) restriction enzyme recognizes GG^CGCGCC sites and cuts best at 37°C in Tango buffer (Isoschizomers: AscI, PalAI).
Sensitive to Heat Inactivation | Yes |
---|---|
Product Type | Restriction Enzyme |
Enzyme | SgsI (AscI) |
Methylation Sensitivity | CpG Methylation-Sensitive,Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive |
Research Category | Traditional Cloning |
Compatible Buffer | 10X Buffer Tango |
Type IIS RE | No |
Optimal Reaction Temperature | 37°C |
Concentration | 10 U/μL |
Thermo Scientific™ MspI (HpaII) (10 U/μL)
The MspI (HpaII) restriction enzyme recognizes C^CGG sites and cuts best at 37°C in Tango buffer.
Sensitive to Heat Inactivation | Yes |
---|---|
Product Type | Restriction Enzyme |
Enzyme | MspI (HpaII) |
Methylation Sensitivity | Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive |
Research Category | Traditional Cloning |
Compatible Buffer | 10X Buffer Tango |
Type IIS RE | No |
Optimal Reaction Temperature | 37°C |
Concentration | 10 U/μL |
Thermo Scientific™ OliI (AleI) (10 U/μL)
The OliI (AleI) restriction enzyme recognizes CACNN^NNGTG sites and cuts best at 37°C in R buffer (Isoschizomers: AleI).
Sensitive to Heat Inactivation | Yes |
---|---|
Product Type | Restriction Enzyme |
Enzyme | OliI (AleI) |
Methylation Sensitivity | CpG Methylation-Sensitive,Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive |
Research Category | Traditional Cloning |
Compatible Buffer | 10X Buffer R |
Type IIS RE | No |
Optimal Reaction Temperature | 37°C |
Concentration | 10 U/μL |
Thermo Scientific™ AloI (2 U/μL)

Cut at ^(7/12-13)GAAC(N)6TCC(12-13/7)^ sites with AloI restriction enzyme, which performs best at 30°C in R buffer.
Thermo Scientific™ MreI (Sse232I) (10 U/μL)

Cut at CG^CCGGCG sites with MreI (Sse232I) restriction enzyme, which performs best at 37°C in G buffer.
Thermo Scientific™ Bovine Serum Albumin (BSA)

Stabilize enzymes, increase PCR yields, and prevent adhesion of enzymes with Thermo Scientific™ Bovine Serum Albumin.

Thermo Scientific™ ATP Solution (100 mM)

Thermo Scientific ATP (adenosine 5'-triphosphate) is an extremely stable nucleotide, and it is supplied as 100 mM aqueous solution titrated to pH 7.3-7.5 with NaOH.

Thermo Scientific™ FastDigest NdeI
The FastDigest NdeI restriction enzyme recognizes CA^TATG sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: FauNDI).

Sensitive to Heat Inactivation | Yes |
---|---|
Product Type | Restriction Enzyme |
Enzyme | Nde I |
Methylation Sensitivity | Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive |
Research Category | Traditional Cloning |
Compatible Buffer | 10X FastDigest Buffer/FastDigest Green Buffer |
Overhang | 5' protruding ends |
Type IIS RE | No |
Optimal Reaction Temperature | 37°C |
Inactivation Temperature | 65°C |
Product Line | FastDigest™ |
Thermo Scientific™ FastDigest MspI

Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer.

Thermo Scientific™ Reaction Buffer with MgCl2 for DNase I (10X)

Optimize reaction condions for RNase-free DNase I with this 10X reaction Tris-HCl (pH 7.5) buffer containing MgCl2.
