Substrates
![Alkali Scientific D Luciferin Free Acid [4, 5 Dihydro 2 (6 hydroxy 2 benzothiazoyl) 4 thiazole carboxylic acid], 1 G](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000660397.jpg-250.jpg)
Alkali Scientific D Luciferin Free Acid [4, 5 Dihydro 2 (6 hydroxy 2 benzothiazoyl) 4 thiazole carboxylic acid], 1 G
D-Luciferin Free Acid (1g) is a highly sensitive bioluminescence substrate used for a range of biochemical assays, particularly in high-sensitivity detection systems. The substrate undergoes oxidation by the enzyme luciferase, producing light, which can be measured and quantified. This 1g product is designed for high-throughput applications, offering excellent sensitivity and reliable results in detecting ATP or monitoring various biological activities. It is widely used in molecular biology, environmental monitoring, and pharmacology research for its ability to generate strong luminescent signals in assays involving cellular or enzymatic processes.
![Alkali Scientific D Luciferin Sodium Salt [4,5 Dihydro 2 (6 hydroxy 2 benzothiazolyl) 4 thia zolecarboxylic acid, sodium salt], 50 Milligrams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000660398.jpg-250.jpg)
Alkali Scientific D Luciferin Sodium Salt [4,5 Dihydro 2 (6 hydroxy 2 benzothiazolyl) 4 thia zolecarboxylic acid, sodium salt], 50 Milligrams
D-Luciferin Sodium Salt (50mg) is a bioluminescence substrate commonly used in high-sensitivity detection assays. When oxidized by luciferase, it generates light, enabling the detection of ATP and the measurement of cellular activity. The sodium salt form of D-Luciferin is favored for applications that require stable light emission, making it essential in biological assays, including ATP monitoring, enzyme activity studies, and cell viability tests. This product is particularly useful in molecular biology, environmental testing, and pharmaceutical research, providing excellent luminescent signals for quantification in high-throughput screening systems.
CHEMPEP INC Ubiquitin-AMC, 100 μg, Fluorogenic substrate for DUB activity assays, C388H636N106O119S, Molecular Weight 8722.2 g/mol, Fluorescence Excitation 380 nm, Emission 460 nm
Ubiquitin-AMC is a highly sensitive fluorogenic substrate essential for measuring deubiquitinating enzyme (DUB) activity in biochemical assays. This versatile reagent, composed of ubiquitin conjugated to the AMC fluorophore, facilitates real-time monitoring of DUB activity by releasing a fluorescent signal. It is particularly advantageous for high-throughput screening and detailed kinetic studies in various cellular processes.
- 100 μg quantity
- Molecular formula: C388H636N106O119S
- Molecular weight: 8722.2 g/mol
- Fluorogenic substrate type
- Target enzyme class: Deubiquitinating enzymes (DUBs)
- Real-time monitoring capability
- High sensitivity for biochemical research
- Uses aqueous buffers for solubility
- Storage temperature: -20°C or below
- Avoid repeated freeze-thaw cycles
- Purity of ≥95%
- Synthetic source of ubiquitin
- Supplied as lyophilized powder or solution in DMSO
Chem-Impex International, Inc. D-Luciferin potassium salt | MFCD00044928 | 100MG
D-Luciferin potassium salt, MFCD00044928, 100MG
Medchemexpress LLC Glp-Asn-Pro-AMC | 291752-43-7 | 99.3% | 497.50 | 25 MG
Glp-Asn-Pro-AMC is identified as an inhibitor of thyrotropin-releasing hormone degrading enzyme (TRH-DE), exhibiting a Ki value of 0.97 μM.
- Inhibitor of thyrotropin-releasing hormone degrading enzyme (TRH-DE)
Enzo Life Sciences FLUOR DE LYS SIRT2 deacetylase substrate (0.5µmol)
FLUOR DE LYS-SIRT2 is a fluorogenic peptide substrate for SIRT2 (human Sirtuin 2). Based on residues 317-320 of p53 (Gln-Pro-Lys-Lys(Ac)), a site of regulatory acetylation by the PCAF acetyltransferase (lysine 320), it was the best for SIRT2 from among a panel of substrates patterned on p53, Histone H3, and Histone H4 acetylation sites. FLUOR DE LYS-SIRT2 deacetylase substrate is deacetylated by SIRT2 at a rate of more than 50-fold that of the acetylated lysine substrate (BML-KI104; acetylated substrates both at 25 µM, 500 µM NAD+). Must be used in conjunction with FLUOR DE LYS Developer II (BML-KI176). Sufficient for 100 assays of human recombinant SIRT2 (BML-SE251; 2U/well, 100 µM substrate). Purity: ≥95% (HPLC). Formulation: Supplied as a 5mM solution (100 µl) in HDAC Assay Buffer. Long Term Storage: -80°C.
Enzo Life Sciences PKC zeta substrate (1mg)
This peptide sequence, RRFKRQGSFFYFF, was selected from a peptide library for optimal phosphorylation by protein kinase C ζ (PKCζ). Alternative name: Protein kinase C zeta substrate. Long Term Storage: -20°C.
Enzo Life Sciences MMP-substrate (fluorogenic) (1mg)
Fluorogenic substrate for several matrix metalloproteinases (MMPs). It is cleaved by MMP-1, MMP-3, MMP-7, MMP-8, MMP-9, MMP-11, MMP-12, MMP-13, and ADAM9/MDC9, but only slightly by MMP-2 and not at all by ADAM17/TACE. Ex/Em= 340nm/440nm, although 365/450 is also appropriate. Highly quenched, with strong fluorescence by Nma at 440nm once cleavage separates it from the aromatic Dnp moiety. Compatible with most fluorometers. Alternative name: Matrix metalloproteinase substrate, Dnp-Pro-beta-cyclohexyl-Ala-Gly-Cys(Me)-His-Ala-Lys(Nma)-NH2. Purity: ≥98% (HPLC). Solubility: Soluble in DMSO (at least 20mM). Long Term Storage: -20°C.
Medchemexpress LLC Z-Gly-Gly-Arg-AMC | 66216-78-2 | 99.9% | 579.60 | 50 MG
Z-Gly-Gly-Arg-AMC is a thrombin-specific fluorogenic substrate used for testing thrombin generation in PRP and platelet-poor plasma (PPP) (Ex/Em = 390/480 nm).
- Thrombin-specific fluorogenic substrate.
- Suitable for testing thrombin generation in PRP and platelet-poor plasma (PPP).
- Excitation/Emission wavelengths: 390/480 nm.
Enzo Life Sciences Shp 1 & 2 substrate (1mg)
This peptide sequence, EFpYAEVGRSPPDPAK, was selected by phage display analysis for optimal Shp-1 and Shp-2 phosphatase activities. Purity: ≥95% (HPLC). Formulation: Lyophilized. Solubility: Soluble in water. Long Term Storage: -20°C.
Enzo Life Sciences Z-LR-AMC (10mg)
Sensitive fluorogenic substrate for cathepsin K (Km=8µM, kcat/Km=4 x 10 M-1s-1). This substrate is also cleaved by cathepsin B, F, L, L2/V, S. Z-LR-AMC is also cleaved by malaria parasite cysteine proteases falcipain-1, -2, -3, berghepain, and vivapain-2, -3. Ex.: 365nm, Em.: 440nm, although the following Ex/Em can also be used: 355,380/450,460. This substrate is useful for inhibitor screening and kinetic analysis. Also available: fluorogenic calibration standard AMC (BML-KI144). Alternative name: Z-Leu-Arg-AMC, Cathepsin K substrate (fluorogenic). Purity: ≥97% (HPLC). Formulation: Lyophilized. Solubility: Soluble in DMSO to at least 20mM. Long Term Storage: -20°C.