Substrates
Invitrogen™ C12FDG (5-Dodecanoylaminofluorescein Di-β-D-Galactopyranoside)
Produces a green fluorescence
Invitrogen™ CMAC, t-BOC-Leu-Met (7-Amino-4-Chloromethylcoumarin, t-BOC-L-Leucyl-L-Methionine amide)
Cleavage produces blue-fluorescence
Invitrogen™ DDAO galactoside (9H-(1,3-Dichloro-9,9-Dimethylacridin-2-One-7-yl) β-D-Galactopyranoside)
The galactosidase substrate, DDAO galactoside yields a hydrolysis product that can be excited with the 633 nm laser (excitation/emission maxima ∼645/660).
Invitrogen™ X-Gal (5-Bromo-4-Chloro-3-Indolyl β-D-Galactopyranoside)
Widely used chromogenic substrate for β-galactosidase
Invitrogen™ DiFMUP (6,8-Difluoro-4-Methylumbelliferyl Phosphate)
Continuously assay acid phosphatases at low pH
Invitrogen™ CDP-Star™ Substrate (12.5 mM Concentrate)
This CDP-Star™ chemiluminescent substrate for alkaline phosphatase let you detect alkaline phosphatase and alkaline phosphatase-labeled molecules with unparalleled sensitivity, speed, and ease.
Invitrogen™ Alexa Fluor™ 647-Poly-GT Peptide Substrate
A general AlexaFluor647-labeled peptide substrate consisting of a polymer of glutamate, and tyrosine that is used to monitor tyrosine kinase acitivty.
BD AEC Substrate Kit
Substrate for peroxidase and is a widely used chromogen for immunohistochemical staining and immunoblotting
Invitrogen™ Nitro blue Tetrazolium Chloride (NBT)
Use to assess cell viability as function of redox potential
Invitrogen™ DQ™ Collagen, type IV From Human Placenta, Fluorescein Conjugate
Analog of the natural substrate
Invitrogen™ DQ™ Collagen, type I From Bovine Skin, Fluorescein Conjugate
The fluorogenic DQ™ collagen can be used to directly monitor collagenase activity.
Invitrogen™ ELF™ 97 Phosphatase Substrate (ELF™ 97 Phosphate), 0.2 μm Filtered
For use with microarray, flow cytometer and fluorescence microscope