Substrates
Enzo Life Sciences FLUOR DE LYS SIRT2 deacetylase substrate (0.5µmol)
FLUOR DE LYS-SIRT2 is a fluorogenic peptide substrate for SIRT2 (human Sirtuin 2). Based on residues 317-320 of p53 (Gln-Pro-Lys-Lys(Ac)), a site of regulatory acetylation by the PCAF acetyltransferase (lysine 320), it was the best for SIRT2 from among a panel of substrates patterned on p53, Histone H3, and Histone H4 acetylation sites. FLUOR DE LYS-SIRT2 deacetylase substrate is deacetylated by SIRT2 at a rate of more than 50-fold that of the acetylated lysine substrate (BML-KI104; acetylated substrates both at 25 µM, 500 µM NAD+). Must be used in conjunction with FLUOR DE LYS Developer II (BML-KI176). Sufficient for 100 assays of human recombinant SIRT2 (BML-SE251; 2U/well, 100 µM substrate). Purity: ≥95% (HPLC). Formulation: Supplied as a 5mM solution (100 µl) in HDAC Assay Buffer. Long Term Storage: -80°C.
Enzo Life Sciences OMNIMMP fluorogenic substrate (1mg)
Fluorogenic substrate for nearly all matrix metalloproteinase (MMP) enzymes, and ADAM17/TACE. This highly-quenched (99.5%) substrate is useful for inhibitor screening and kinetic analysis. Ex.: 328 nm, Em.: 393 nm, although the following Ex/Em have been used: 320-340/400-420. Also available: OMNIMMP Fluorogenic Control Peptide (BML-P127). Alternative name: Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 . AcOH, Matrix metalloproteinase substrate. Purity: ≥98%. Solubility: Soluble in methanol (1mg/ml) or DMSO (20mg/ml). Long Term Storage: -20°C. Applications: WB Assay.
Enzo Life Sciences Chk 1 & 2 substrate (1mg)
This peptide sequence, KKKVSRSGLYRSPSMPENLNRPR, corresponds to amino acids 206-225 of the phosphatase CDC25C, which is a phosphorylation target of Chk1 on Ser216. The addition of three N-terminal lysines allows the peptide to be used in phosphocellulose kinase assays. Both Chk1 and Chk2 will phosphorylate this peptide. Alternative name: CDC25C (206-225). Long Term Storage: -20°C.
Sino Biological Lipid Substrates: DLG (Dilauroyl-sn-glycerol)
The substrate solution used for assaying Diacylglycerol kinases.
Gold Biotechnology Inc D-Luciferin, Potassium Salt (Proven and Published™) 100 mg
Luciferin is a common bioluminescent reporter used for in vivo imaging of the expression of luciferase. This water soluble substrate for the firefly luciferase enzyme utilizes ATP and Mg2+ as cofactors to emit a characteristic yellow-green emission in the presence of oxygen, which shifts to red light in vivo at 37°C. Through the utilization of ATP, the reaction can be further used to indicate the presence of energy or life in order to function as a life-death stain.
Medchemexpress LLC Boc-LRR-AMC | 5MG
Boc-LRR-AMC (Boc-Leu-Arg-Arg-AMC) is a fluorogenic substrate which can be utilized for detecting the trypsin-like activity of the 26S proteasome or the 20S proteasome core[1]
Cayman Chemical Boc-Lys Ac-AMC 50mg
A fluorogenic HDAC substrate; ex/em = 340-360/440-460 nm, respectively; following deacetylation by an HDAC and trypsin cleavage, AMC is released and its fluorescence can be used to quantify HDAC activity
Ubpbio A FRET-based fluorogenic substrate of Papain-like protease (PLPro) from SARS viruses, including SARS-CoV-2. PLPro also has deubiquitinase activity. 5MG
Dabcyl–FRLKGGAPIKGV–Edans is an internally quenched fluorogenic substrate for the SARS-CoV or MERS-CoV family Papain-like protease (PLPro or PLP) that recognizes a LXGG consensus cleavage sites. Upon PLP-mediated cleavage between glycine and alanine, the resulting APIKGV-Edans fluorescence can be monitored at the excitation/emission wavelengths of 355/538 nm, respectively. In addition, PLP has deubiquitinating and deISG15ylation activities.
Ubpbio K63-linked hexa-Ubiquitin (human), enzymatically synthesized
Ub chains are formed by conjugating the C-terminal glycine residue of Ub onto any of seven internal lysine residues or the amino group of the previous Ub. Ub chains are classified by the lysine residue used to link Ubs; different Ub chain topologies can result in different signals. For instance, Ub chains linked through lysine 6, 11, 27, 29, 33 and 48 are capable of targeting proteins for proteasomal degradation; in contrast, Ub chains linked through lysine 63 or the N-terminal amino group (linear Ub chains) often play important nonproteolytic functions including regulation of kinase activation and protein translation. All Ub chain products are produced by using of human wild type Ub reacting with specific E2s.
Abcam L-alpha-Phosphatidylinositol, Phospholipase C substrate, 1G
MW 867.14 g/mol. Phospholipase C substrate. Phospholipid membrane component. Inositol phosphate precursor. Stimulates vascular Ca²+-dependent protease autolysis.
The product is subject to the following: Abcam Restricted Use Statement
Abcam Cytidine 5'-diphosphate (CDP) sodium salt hydrate, CDP kinase substrate, 500MG
MW 469.18 g/mol, Purity >98%. CDP (nucleoside diphosphate) kinase substrate to produce CTP in support of DNA and RNA biosynthesis. Ribonucleotide reductase substrate to produce dCMP.
The product is subject to the following: Abcam Restricted Use Statement