Substrates
Research Products International Corp 1-Naphthyl Phosphate Monosodium Salt Monohydrate, 25 Grams
Substrate for determaination of phosphatase. Protect from moisture.
Biotium Coelenterazine (ALSO KNOWN AS NATIVE COE
Coelenterazines are luminescent enzyme substrates for apoaequorin and Renilla luciferase. Apoaequorin is used as a calcium indicator whereas Renilla luciferase is commonly used as a reporter of transcription regulation. Coelenterazine binds apoaequorin to form an aequorin complex which emits light upon calcium binding, and can measure a broad range of calcium concentrations from ~0.1 uM to >100 uM. Renilla luciferase is widely used as a reporter protein and as a donor in bioluminescence resonance energy transfer (BRET) to study protein-protein interactions. Coelenterazines can also be used for chemiluminescent detection of superoxide anion and peroxynitrite in cells or tissues.Coelenterazine (native): Yellow solid soluble in MeOH or EtOH; Store at -20°C and protect from light; C26H21N3O3; MW: 423; [55779-48-1]. See also other Coelentrazine analogs and water-soluble Aquaphile™ Coelenterazines (native and analog h) for in vivo use.
Sigma Aldrich Fine Chemicals Biosciences D Luciferin synthetic50MG
D-Luciferin is a small molecule present abundantly in bioluminescent organisms. This molecule is sensitive to oxygen and light.
Sigma Aldrich Fine Chemicals Biosciences Adenosine-5'-diphosphoglucose disodium salt >=93% | Purity: >=93% | Mol Wt: 633.31 | 102129-65-7 | MFCD00056005 | 100MG
Adenosine-5'-diphosphoglucose disodium salt >=93% | 102129-65-7 | MFCD00056005 | 100MG
Sino Biological Assay Buffer and Co-factors: DUB Reaction Buffer (4X) 150 µL
The working solution used for diluting DUBs.
Apexbio Technology LLC AMC-100MG
AMC (CAS No 26093-31-2) is a compound prevalent in biomedical research especially in the study of enzymatic activity Originating from the need to understand biological pathways AMC serves as a substrate in assays measuring enzyme kinetics and activity It is commonly used in studies involving serine proteases and other peptidases due to its ability to release a fluorescent signal upon enzymatic cleavage allowing researchers to monitor real-time enzyme activity Its effectiveness is often observed in nanomolar to low micromolar ranges making it suitable for high-throughput screening applications AMC is frequently applied in cell models and animal studies to evaluate enzyme inhibitors and explore potential therapeutic pathways Experimental concentrations typically depend on the specific research design highlighting its adaptability in various scientific investigations