Microplate Readers and Reader Accessories
Microplate readers are a type of spectrophotometer designed specifically for analyzing multiple samples in multi-well plates.
The readers detect light signals that result from biological, chemical, biochemical, or physical reactions. The signals are produced by, converted by, or transmitted through the samples and are measured by a detector. The detectors are usually photomultiplier tubes (PMT), which convert the released photons into electricity. The current is quantified by the reader’s software to produce the numerical result.
In some cases, samples are first excited by exposure to light of specific wavelengths. Filters or monochromators are used to select or restrict the wavelengths needed for emission or detection.
Single-mode microplate readers commonly measure absorbance, fluorescence, or luminescence. Multi-mode plate readers may measure absorbance, fluorescence, and luminescence with options for fluorescence polarization (FP). They may also include lasers for time-resolved fluorescence (TRF), TR-FRET, Amplified Luminescent Proximity Homogeneous Assays (ALPHA), and cell imaging.
Features may include:
- Monochromator- or filter-based wavelength selection
- Visible only or UV/Vis spectrum, fluorescence, and luminescence
- Multiple photomultiplier tubes (PMTs) for simultaneous assays
- Temperature and CO2/O2controls
- Modular design for adding functionality
Microplate readers are used for high-throughput screening (HTS), biomarker assays, nucleic acid and protein quantification, kinetics, ELISA assays, homogeneous time resolved fluorescence (HTRF) for measuring cytokines, bioluminescence resonance energy transfer (BRET), and other techniques.
Alkali Scientific ASI™ Confocal Dish 35 x10mm, Clear, Easy-Grip, Insert Type, Sterile, PS/Glass, Non-Treated (Non-Charged/ Hydrophobic), 500/Cs
ASI™ product with a coverglass bottom is easy to use and facilitates preparation of cells for microscopic analysis. Optically clear with low background fluorescence coverglass was used. Ideal for use in high resolution and inverted microscopy, fluorescence imaging in live cells, confocal microscopy, phase contrast microscopy and micro-manipulations. ▪ Dish size : 35 mm Plate size: 6well plate(Cat.NO. 30106, 30206)▪ Hole size : 13 Øor 20 Ø▪ Coverglass size : 22 x 22 mm(adhesion type, hole 13 Ø), 24 x 24(insert and adhesion type, hole 20 Ø)▪ Coverglass thickness : 0.13 ~ 0.16 mm▪ External grip 35 mm dish are available▪ Black color 35mm dish is also available▪ Sterilized by gamma irradiation▪ Polystyrene
Alkali Scientific ASI™ Black Confocal Dish 35 x10mm, Easy-Grip, Adhesion Type, Sterile, Treated (Positive-Charged/ Surface Coated), 50/Cs, 22 x 22 x 0.13mm, Hole 3.14mm, PS/Flux
ASI™ confocal products allow researchers to acquire high resolution microscopic images of cells in 35 mm culture dishes or 6 well plates. Low auto-fluorescence glass and FLux were used, ideal for uses of these products are confocal microscopy, phase contrast microscopy, live cell imaging and micro-manipulations. Surface materials for confocal products:Glass: naturally charged, good growth surface for adherent cellsFLux: surface modified plastic film (188 μm thick), optimal for confocal microscopy Bottom materials: Glass/FLux (no surface treatment for glass bottom) Coverslip: #1.5 Dish color: Clear/Black Dish size: 35 Ø Hole size: 13 Ø / 20 Ø / 25Ø Non – pyrogenic Non – cytotoxic DNase / RNase – free Human DNA – free
Agilent Technologies Seahorse XF24 V7 PS Cell Culture Microplates. For use with both Seahorse XFe24 and XF24 Analyzers,XF cell culture microplates are tissue culture treated for optimum adherence and growth of mammalian cells. The XF microplates are supplied in individual gamma irradiated conta...
Seahorse XF24 V7 PS Cell Culture Microplates. For use with both Seahorse XFe24 and XF24 Analyzers,XF cell culture microplates are tissue culture treated for optimum adherence and growth of mammalian cells. The XF microplates are supplied in individual gamma irradiated containers. 10/pk
Alkali Scientific ASI™ 6-Well Confocal Plate (35m), Clear, Adhesion Type, Sterile, 4/Cs, PS/Flux, Treated (Positive-Charged/ Surface Coated), 50/Cs, 22 x 22 x 0.13mm, Hole 3.14mm
ASI™ confocal products allow researchers to acquire high resolution microscopic images of cells in 35 mm culture dishes or 6 well plates. Low auto-fluorescence glass and FLux were used, ideal for uses of these products are confocal microscopy, phase contrast microscopy, live cell imaging and micro-manipulations. Surface materials for confocal products: Glass: naturally charged, good growth surface for adherent cellsFLux: surface modified plastic film (188 μm thick), optimal for confocal microscopy ▪ Bottom materials: Glass/FLux (no surface treatment for glass bottom)▪ Dish color: Clear▪ Dish size: 35 Ø▪ Plate size: 6well Plate▪ Hole size: 13 Ø / 20 Ø / 25Ø▪ Non – pyrogenic▪ Non – cytotoxic▪ DNase / RNase – free▪ Human DNA – free
Molecular Devices Fluorescence Filter, 740 nm, 50 nm, With Holder
Fluorescence interference filter supplied with a mounting holder for insertion into compatible filter-slide microplate readers. Intended for near-infrared fluorescence detection in microplate assays.
- Center wavelength 740 nm.
- Bandwidth 50 nm.
- Includes mounting holder for filter slides.
- Designed for fluorescence detection; suitable for luminescence and TRF read modes.
- Intended for microplate and ELISA assays using filter-based readers.
- Interference filter construction for stable optical performance.
Molecular Devices Low-Volume Slide Refill Kit, 64-Well, Pack of 3
Replenishment kit of low-volume bottom slides for slide-based microplate readers. The kit includes three low-volume bottom slides, each with 64 wells, intended for low-volume absorbance and related assays.
- Contains three 64-well low-volume bottom slides.
- Intended for low-volume absorbance and slide-based assays.
- Supplies replacement slides to restore slide reader capacity.
Molecular Devices Microplate Reader, Fluorescence, Dual Monochromator, 250-850 nm
The Gemini XPS microplate reader is a top-reading fluorescence spectrofluorometer that provides flexible excitation and emission selection, sensitive detection, and multiple read modes for microplate assays.
- Dual monochromators for excitation and emission wavelength selection.
- Excitation range 250-850 nm.
- Emission range 360-850 nm.
- Supports 6, 12, 24, 48, 96, and 384-well microplates.
- Read types: endpoint, kinetic, spectral scan, and well scan.
- Xenon flash lamp light source.
- Photomultiplier tube detector.
- Temperature control from ambient +4°C to 45°C.
- Approximate weight 35 lb (16 kg) and dimensions 34 x 42 x 42 cm.
Electron Microscopy Sciences NIGHTSEA™ Stereo Fluorescence Add-On Light and Filter Set, Ultra Violet
Additional combinations excitation/ emission wavelength combination can be added NIGHTSEA™ Stereo Microscope Fluorescence Viewing system by purchasing a kit that consists of a light head, barrier filter, and viewing shield. These three elements can be removed and replaced in seconds, and color coding ensures that you are using the right combination. Sets are named and color coded for the color of the excitation light, not the color of the emitted fluorescence.
Color: Ultra Violet
Excitation: 360-380nm
Emission filter: 415 nm longpass
Fluorophores: DAPI
Medchemexpress LLC Tofacitinib 10 mM * 1 mL in DMSO, Solid + Solvent, Ready for Reconstitution
Tofacitinib is an orally available JAK3/2/1 inhibitor with IC50s of 1, 20, and 112 nM, respectively.
CERILLO INC Alto Plate Reader, 600nm
The Alto, 600nm includes one Alto plate reader, one USB cable, one power adapter, one 16 GB microSD card, and 1 year of firmware upgrades.
Revvity Health Sciences Inc HTRF Human Fibronectin EDA Detection Kit, 10,000 Assay Points
HTRF Human Fibronectin EDA Detection Kit, 10,000 Assay Points
Sigma Aldrich Fine Chemicals Biosciences Duolink(R) In Situ Wash Buffers, Fluorescence | 20L
Duolink(R) In Situ Wash Buffers, Fluorescence | 20L