Microplate Readers and Reader Accessories
Microplate readers are a type of spectrophotometer designed specifically for analyzing multiple samples in multi-well plates.
The readers detect light signals that result from biological, chemical, biochemical, or physical reactions. The signals are produced by, converted by, or transmitted through the samples and are measured by a detector. The detectors are usually photomultiplier tubes (PMT), which convert the released photons into electricity. The current is quantified by the reader’s software to produce the numerical result.
In some cases, samples are first excited by exposure to light of specific wavelengths. Filters or monochromators are used to select or restrict the wavelengths needed for emission or detection.
Single-mode microplate readers commonly measure absorbance, fluorescence, or luminescence. Multi-mode plate readers may measure absorbance, fluorescence, and luminescence with options for fluorescence polarization (FP). They may also include lasers for time-resolved fluorescence (TRF), TR-FRET, Amplified Luminescent Proximity Homogeneous Assays (ALPHA), and cell imaging.
Features may include:
- Monochromator- or filter-based wavelength selection
- Visible only or UV/Vis spectrum, fluorescence, and luminescence
- Multiple photomultiplier tubes (PMTs) for simultaneous assays
- Temperature and CO2/O2controls
- Modular design for adding functionality
Microplate readers are used for high-throughput screening (HTS), biomarker assays, nucleic acid and protein quantification, kinetics, ELISA assays, homogeneous time resolved fluorescence (HTRF) for measuring cytokines, bioluminescence resonance energy transfer (BRET), and other techniques.
Molecular Devices Microplate Reader, Multi-Mode, 8-Channel, 2 to 45 °C
The FlexStation 3 Multi-Mode Microplate Reader is a benchtop multimode instrument for kinetic and endpoint assays with integrated liquid handling and temperature control. It measures absorbance, fluorescence intensity, fluorescence polarization, luminescence, and time-resolved fluorescence using monochromators for flexible wavelength selection.
- Integrated 8-channel pipettor for timed reagent additions.
- Supports 6, 12, 24, 48, 96, and 384 well plate formats.
- Detection modes include absorbance, fluorescence intensity, fluorescence polarization, luminescence, and time-resolved fluorescence.
- Monochromators tunable in 1.0 nm increments for flexible wavelength selection.
- Wavelength coverage suitable for absorbance and fluorescence assays.
- Temperature control from 2°C above ambient to 45°C for incubated assays.
- Suitable for kinetic assays, compound additions, and high-throughput workflows.
Molecular Devices Absorbance Filter, 260 nm, 5.5 nm Bandwidth
The Molecular Devices 260 nm absorbance filter (5.5 nm bandwidth) is an optical bandpass accessory designed for use with compatible FilterMax series multimode microplate readers. It provides a narrow UV transmission centered at 260 nm for precise absorbance measurements of nucleic acids and other UV-absorbing analytes.
- Provides narrow bandpass transmission centered at 260 nm.
- 5.5 nm bandwidth for selective UV absorbance measurements.
- Suitable for nucleic acid quantification and other UV-absorbing samples.
- Designed for easy installation in modular filter holders.
- Improves measurement specificity by reducing out-of-band light.
Molecular Devices Multi-Mode Microplate Reader, Monochromator, 6-384 Well, 200-1000 nm
The SpectraMax M3 is a multi-mode microplate reader for absorbance, fluorescence, and luminescence assays. It combines dual monochromator optics, a dedicated luminescence PMT, integrated temperature control, and is operated with preloaded data acquisition and analysis software.
- Measures absorbance (200-1000 nm), fluorescence, and luminescence (250-850 nm).
- Compatible with 6, 12, 24, 48, 96, and 384-well microplates.
- Dual monochromators with 1 nm wavelength increments for precise selection.
- Dedicated PMT for low-noise luminescence detection and high fluorescence sensitivity.
- Integrated temperature control from ambient +2°C to 60°C with ±1°C accuracy.
- Xenon flash lamp light source and programmable orbital shaker for assay mixing.
- Robotics compatible design for automation and high-throughput workflows.
Molecular Devices Excitation Filter, 585 nm, 10 nm Bandwidth
This excitation filter provides narrowband fluorescence excitation centered at 585 nm with a 10 nm full width, for use in compatible multi-mode microplate readers.
- Center wavelength 585 nm.
- Bandwidth 10 nm full width.
- Excitation filter type suitable for narrowband fluorescence assays.
- Designed for installation in standard filter slide assemblies.
Molecular Devices Microplate Reader, Multi-Mode, Dual Monochromators, 6-384-Well
The SpectraMax M5e is a modular multi-mode microplate reader designed for a wide range of life science assays. It provides flexible wavelength selection and sensitive detection across multiple modes while supporting automation for increased throughput.
- Performs absorbance, fluorescence intensity, luminescence, time-resolved fluorescence, and fluorescence polarization measurements.
- Dual scanning monochromators with 1 nm spectral scanning for flexible excitation and emission selection.
- Spectral ranges: absorbance 200-1000 nm; fluorescence/luminescence/TRF 250-850 nm; fluorescence polarization 300-750 nm.
- Supports 6-384 well microplates and cuvette-based measurements.
- Compatible with automated plate handling, including a StakMax stacker with up to 50-plate capacity and optional barcode reader.
- Includes PathCheck sensor for absorbance normalization and AutoPMT optimization for extended dynamic range.
Molecular Devices Fluorescence Polarization Filter, 485 nm, 20 nm, Vertical
The Molecular Devices fluorescence polarization filter (MPN 6590-0094) is a vertical polarization filter engineered for fluorescence polarization assays with a 485 nm center wavelength and a 20 nm optical bandwidth. It is intended as a replacement or accessory filter for compatible multi-mode microplate readers.
- Optimized for fluorescence polarization assays at a 485 nm center wavelength.
- 20 nm bandwidth provides narrow spectral selection and reduced crosstalk.
- Vertical polarization orientation for polarization-based measurements.
- Designed for installation in filter-slide assemblies of compatible microplate readers.
- Replacement filter for restoring or modifying detection channels.
Alkali Scientific ASI™ Black Confocal Dish 35 x10mm, Easy-Grip, Adhesion Type, Sterile, Non-Treated (Non-Charged/ Hydrophobic), 100/Cs, 22 x 22 x 0.13mm, Hole 1.33mm, PS/Glass
ASI™ confocal products allow researchers to acquire high resolution microscopic images of cells in 35 mm culture dishes or 6 well plates. Low auto-fluorescence glass and FLux were used, ideal for uses of these products are confocal microscopy, phase contrast microscopy, live cell imaging and micro-manipulations. Surface materials for confocal products:Glass: naturally charged, good growth surface for adherent cellsFLux: surface modified plastic film (188 μm thick), optimal for confocal microscopy Bottom materials: Glass/FLux (no surface treatment for glass bottom) Coverslip: #1.5 Dish color: Clear/Black Dish size: 35 Ø Hole size: 13 Ø / 20 Ø / 25Ø Non – pyrogenic Non – cytotoxic DNase / RNase – free Human DNA – free
Revvity Health Sciences Inc HTRF Human Total IκKα Detection Kit, 50,000 Assay Points
HTRF Human Total IκKα Detection Kit, 50,000 Assay Points
Revvity Health Sciences Inc HTRF Human Total KRAS Detection Kit, 50,000 Assay Points
HTRF Human Total KRAS Detection Kit, 50,000 Assay Points
Revvity Health Sciences Inc HTRF Human CCL22 Detection Kit, 100,000 Assay Points
HTRF Human CCL22 Detection Kit, 100,000 Assay Points
Perkin Elmer US LLC Hangdown Wire Alignment Tool
This tool ships with the TGA 8000 Spares Kit and the PYRIS 1 TGA with Autosampler Spares Kit.
Molecular Devices Fluorescence Polarization Filter, 485 nm, 20 nm, Horizontal
Fluorescence polarization filter with holder designed for fluorescence polarization assays. The filter provides excitation at 485 nm with a 20 nm bandwidth and a horizontal polarized orientation. It installs in standard filter slides used by multi-mode microplate readers.
- Excitation center wavelength 485 nm with 20 nm bandwidth.
- Polarized horizontal orientation for fluorescence polarization assays.
- Includes filter holder compatible with standard filter slides.
- Intended for use with multi-mode microplate readers in fluorescence polarization workflows.
- Suitable for assays requiring excitation near 485 nm.