Microplate Readers and Reader Accessories
Microplate readers are a type of spectrophotometer designed specifically for analyzing multiple samples in multi-well plates.
The readers detect light signals that result from biological, chemical, biochemical, or physical reactions. The signals are produced by, converted by, or transmitted through the samples and are measured by a detector. The detectors are usually photomultiplier tubes (PMT), which convert the released photons into electricity. The current is quantified by the reader’s software to produce the numerical result.
In some cases, samples are first excited by exposure to light of specific wavelengths. Filters or monochromators are used to select or restrict the wavelengths needed for emission or detection.
Single-mode microplate readers commonly measure absorbance, fluorescence, or luminescence. Multi-mode plate readers may measure absorbance, fluorescence, and luminescence with options for fluorescence polarization (FP). They may also include lasers for time-resolved fluorescence (TRF), TR-FRET, Amplified Luminescent Proximity Homogeneous Assays (ALPHA), and cell imaging.
Features may include:
- Monochromator- or filter-based wavelength selection
- Visible only or UV/Vis spectrum, fluorescence, and luminescence
- Multiple photomultiplier tubes (PMTs) for simultaneous assays
- Temperature and CO2/O2controls
- Modular design for adding functionality
Microplate readers are used for high-throughput screening (HTS), biomarker assays, nucleic acid and protein quantification, kinetics, ELISA assays, homogeneous time resolved fluorescence (HTRF) for measuring cytokines, bioluminescence resonance energy transfer (BRET), and other techniques.
Revvity Health Sciences Inc HTRF Human Total Estrogen Receptor α Detection Kit, Detection Kit, Control Lysate
HTRF Human Total Estrogen Receptor α Detection Kit, Detection Kit, Control Lysate
Molecular Devices Absorbance Filter, 640 nm, 8 nm BW
The AD 340 absorbance photometric filter provides a narrowband 640 nm passband for absorbance measurements in filter-wheel microplate readers. It is supplied as a single replacement filter for compatible readers and supports assays that require red-wavelength detection.
- Absorbance detection at 640 nm center wavelength.
- Bandwidth 8 nm.
- Designed for filter-wheel microplate reader configurations.
- Single replacement filter for maintenance and assay reproducibility.
- Supports red-wavelength absorbance assays and endpoint measurements.
Revvity Health Sciences Inc HTRF Human IRF5 Detection Kit, Control Lysate
HTRF Human IRF5 Detection Kit, Control Lysate
Revvity Health Sciences Inc HTRF Human & Mouse Total HDAC4 Detection Kit, Control Lysate
HTRF Human & Mouse Total HDAC4 Detection Kit, Control Lysate
Revvity Health Sciences Inc AlphaLISA SureFire Ultra Multiplex Human Phospho-CDK1 (Tyr15 & Total) Detection Kit, 100 Assay Points
AlphaLISA SureFire Ultra Multiplex Human Phospho-CDK1 (Tyr15 & Total) Detection Kit, 100 Assay Points
Revvity Health Sciences Inc HTRF cIAP1 BIR2 Binding Kit, 100,000 Assay Points
HTRF cIAP1 BIR2 Binding Kit, 100,000 Assay Points
Revvity Health Sciences Inc HTRF Human Total JAK3 Detection Kit, Control Lysate
HTRF Human Total JAK3 Detection Kit, Control Lysate
Revvity Health Sciences Inc HTRF Human & Mouse Ataxin 2 Detection Kit, 100,000 Assay Points
HTRF Human & Mouse Ataxin 2 Detection Kit, 100,000 Assay Points
Revvity Health Sciences Inc HTRF Human & Mouse GAPDH Housekeeping Kit, 50,000 Assay Points
HTRF Human & Mouse GAPDH Housekeeping Kit, 50,000 Assay Points
Molecular Devices Microplate Reader, Multi-Mode, Tunable Monochromator, 6-384 Well
The SpectraMax iD3 multi-mode microplate reader from Molecular Devices provides absorbance, fluorescence (top and bottom), and luminescence detection for 6-384 well microplates. It features a tunable monochromator with 1-nm increments, intuitive touchscreen control, and software tools for spectral scanning and assay optimization, enabling rapid assay development and reliable quantitative measurements.
- Multi-mode detection for absorbance, fluorescence, and luminescence assays.
- Tunable monochromator with 1-nm wavelength increments for spectral flexibility.
- Broad wavelength coverage for diverse assay types, including UV and visible ranges.
- Supports 6 to 384 well plate formats for assay throughput flexibility.
- Intuitive touchscreen and software tools for quick setup and assay optimization.
- High photometric precision and repeatability for reliable quantitative results.