Microplate Readers and Reader Accessories
Microplate readers are a type of spectrophotometer designed specifically for analyzing multiple samples in multi-well plates.
The readers detect light signals that result from biological, chemical, biochemical, or physical reactions. The signals are produced by, converted by, or transmitted through the samples and are measured by a detector. The detectors are usually photomultiplier tubes (PMT), which convert the released photons into electricity. The current is quantified by the reader’s software to produce the numerical result.
In some cases, samples are first excited by exposure to light of specific wavelengths. Filters or monochromators are used to select or restrict the wavelengths needed for emission or detection.
Single-mode microplate readers commonly measure absorbance, fluorescence, or luminescence. Multi-mode plate readers may measure absorbance, fluorescence, and luminescence with options for fluorescence polarization (FP). They may also include lasers for time-resolved fluorescence (TRF), TR-FRET, Amplified Luminescent Proximity Homogeneous Assays (ALPHA), and cell imaging.
Features may include:
- Monochromator- or filter-based wavelength selection
- Visible only or UV/Vis spectrum, fluorescence, and luminescence
- Multiple photomultiplier tubes (PMTs) for simultaneous assays
- Temperature and CO2/O2controls
- Modular design for adding functionality
Microplate readers are used for high-throughput screening (HTS), biomarker assays, nucleic acid and protein quantification, kinetics, ELISA assays, homogeneous time resolved fluorescence (HTRF) for measuring cytokines, bioluminescence resonance energy transfer (BRET), and other techniques.
Electron Microscopy Sciences S10 Stage Counting Grid 0.05 mm Square
Stage graticules are used for the routine calibration of eyepiece patterns, particularly when alternating between objectives on one microscope or when using the same graticule in different microscopes. Their robust construction makes them ideal for student use and for instructional purposes. The scale or grid is centered on a glass disc mounted in a black anodized aluminum slide 75 mm x 24 mm x 2 mm thick. Transmitted light stage slide with 10 x 10 grid of 0.05mm squares.
Electron Microscopy Sciences USAF Test Chart R75P Positive Image
USAF resolution charts are recognized the world over as a universal standard for testing the vertical and horizontal resolution of imaging systems. Each element on the chart comprises three vertical bars and three horizontal bars, and the detail on these slides is as fine as 0.78microns (644 line-pairs per mm). The resolution of the imaging system is normally specified as the Group and Element of the finest bars that can be clearly defined. Sub Micron smallest feature size - bar width 0.00078mm [0.78 um].
Type: Positive Image
Use: Groups 0/1 to 9/3
Meso Scale Discovery U-PLEX Human YKL-40 ASY
The Meso Scale Discovery U-PLEX Human YKL-40 Assay Kit is an electrochemiluminescent immunoassay for quantifying YKL-40 in human serum plasma and cell culture supernatants It uses the SECTOR Imager 6000 for high sensitivity and a wide dynamic range The kit includes a 96-well plate detection antibody calibrators and diluent It detects as low as 1 15 pg/mL and up to 100 000 pg/mL requires 50 L sample volume and has 10% variation The assay completes in about 3 5 hours
Electron Microscopy Sciences MAC X-Ray Fluorescence Single Standard Nd
These standards are supplied as 1/4" (33 mm) diameter pressed pellets or metal foils, and are prepared from selected high purity elements and compounds to ensure interference free-spectra. They are suitable for use by experienced spectroscopists as well as staff training. Each of the pressed pellets is supported by a thin-walled aluminum cup which is protected from damage during handling and minimizes the risk of touching the standard materials (some of which are harmful).
Fisherbrand™ accuSkan™ FC Filter-Based Microplate Photometer
For research and routine ELISA applications
Fisherbrand™ accuSkan™ FC Filter-Based Microplate Photometer
For research and routine ELISA applications
Agilent BioTek Synergy HTX Multi-Mode Microplate Reader, IVD
The BioTek Synergy HTX is a compact, economical multi-mode reader for 6- to 384-well microplates and Take3 microvolume plates. For In Vitro Diagnostic Use.
Agilent BioTek Gas Controller for CO2 Control
For use with Cytation3 Cell Imaging multi-mode reader and synergy H1 hybrid microplate reader.
