With more than 50,000 citations, Vector Laboratories ABC kits are trusted by immunohistochemistry (IHC) and immunocytochemistry (ICC) laboratories to provide reliable results. All of the VECTASTAIN kits are based on the versatile avidin-biotin complex (ABC) interaction, and different kit variations can be used to accommodate a wide array of primary antibody and tissue species. This kit uses alkaline phosphatase (AP) as a detection enzyme and includes a secondary antibody that targets mouse IgG primary antibodies. The ABC complex, once formed, is stable and can be used for several days after preparation. The VECTASTAIN ABC-AP Staining Kit (Mouse IgG) consists of 3 ml normal horse serum for blocking, 1 ml biotinylated horse anti-mouse IgG secondary antibody, 2 ml Reagent A (avidin) and 2 ml Reagent B (biotinylated AP). Stains 1000-2000 sections.