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Luminescent ATP Cell Viability Assay Kit II assesses cell viability by quantitatively detecting intracellular adenosine triphosphate (ATP) an indicator proportional to metabolically active viable cells The assay utilizes thermostable firefly luciferase-mediated luminescence added reagents lyse cells releasing ATP which then reacts with supplied substrate under luciferase catalysis generating a luminescent signal directly proportional to live cell number This lyophilized formulation offers improved stability at -20 C storage short assay duration ( 10 min) enhanced sensitivity and linear quantification between approximately 10 to 30 000 cells facilitating biomedical assay applications
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Small and Specialty Supplier Partner Small and/or specialty supplier based on Federal laws and SBA requirements. Learn More
Luminescent ATP Cell Viability Assay Kit I uses thermostable firefly luciferase and purified luciferin substrate to quantitatively measure cellular ATP levels an indicator closely correlated with viable cell numbers and metabolic activity Upon reagent addition cells rapidly lyse to release intracellular ATP which interacts with luciferase and luciferin substrate generating a luminescent signal directly proportional to the number of viable cells The protocol does not require prior cell lysis steps offering a streamlined assay completed within 10 minutes This assay is suitable for detecting viability ranging from 10 to 30 000 cells and provides comparable results to common commercial ATP-based viability assays such as CellTiter-Glo
Encompass Procurement Services Non-distribution item offered as a customer accommodation; additional freight charges may apply. Learn More