Proteins and Derivatives
Vector Laboratories Erythrina Cristagalli Lectin (ECL, ECA) Fluorescein-labeled 5 mg
Erythrina cristagalli lectin (ECL, ECA) binds a carbohydrate structure frequently found in mammalian membrane and serum glycoproteins. Sialic acid substitution on this structure prevents binding, which allows the use of agarose-bound ECL to isolate or fractionate mammalian glycoproteins. ECL is useful for isolating human natural killer (NK) cells using negative selection panning. These cells lack accessible carbohydrate structures required for binding ECL and, unlike other mononuclear cells, do not adhere to ECL-coated dishes. High recovery of viable NK cells is possible. Adherent cells can also be recovered by incubation in galactose or lactose. This fluorescein-labeled lectin features a ratio of fluorophores to lectin protein that provides optimal staining (excitation 495 nm, emission 515 nm). Supplied as a solution essentially free of unconjugated fluorophores, it is preserved with sodium azide. The recommended inhibiting/eluting sugar is 200 mM lactose.
Biotium Fibronectin(2755-8), CF405S conjugate, 0.1mg/mL
Fibronectin is an extracellular matrix glycoprotein present on most cell surfaces, in extracellular fluids and in plasma. A high molecular weight heterodimeric protein, it was originally discovered as a protein missing from the surfaces of virus-transformed cells, and it has been shown to be involved in various functions including cell adhesion, cell motility and wound healing. Alternative splicing and glycosylation give rise to several different forms of Fibronectin, some of which exhibit restricted tissue distribution or association with malignancies. It has been shown that Myofibroblasts phenotype formation correlates with the occurrence of glycosylated Fibronectin and Fibronectin splice variants in Dupuytren's disease.Primary antibodies are available purified, or with a selection of fluorescent CF Dyes and other labels. CF Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF405S and CF405M are not recommended for det
Bioworld Bovine Serum Albumin (BSA), Fraction V - Standard Grade, 25 g
BSA (Bovine Serum Albumin), Fraction VProtein with numerous biochemical applications including ELISAs, immunoblots, and immunohistochemistryHeat shock isolationSpecifications:pH (5% water): 6.5-7.5 Heavy Metals (Pb): <0.001% DNase: None detected RNase: None detected Protease: None detectedUsually ships within 24 hours. This product is sold for laboratory use only.
Biotium CD63(MX-49.129.5), CF640R conjugate, 0.1mg/mL
This MAb recognizes protein of 26 kDa-60 kDa, which is identified as CD63. Its epitope is different from that of MAb LAMP3/529. The tetraspanins are integral membrane proteins expressed on cell surface and granular membranes of hematopoietic cells and are components of multi-molecular complexes with specific integrins. The tetraspanin CD63 is a lysosomal membrane glycoprotein that translocates to the plasma membrane after platelet activation. CD63 is expressed on activated platelets, monocytes and macrophages, and is weakly expressed on granulocytes, T cell and B cells. It is located on the basophilic granule membranes and on the plasma membranes of lymphocytes and granulocytes. CD63 is a member of the TM4 superfamily of leukocyte glycoproteins that includes CD9, CD37 and CD53, which contain four transmembrane regions. CD63 may play a role in phagocytic and intracellular lysosome-phagosome fusion events. CD63 deficiency is associated with Hermansky-Pudlak syndrome and is stro