Test
Thermo Fisher Scientific offers bright conjugates of wheat germ agglutinin (WGA) and Alexa Fluor, Alexa Fluor Plus, and other dyes. Fluorescent WGA conjugates bind to carbohydrates and are used for various cell biology applications such as plasma membrane labeling and cell painting assays.
Ligand Type | N-acetylglucosamine and N-acetylneuraminic acid (sialic acid) residues |
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Protein Family | Lectins |
Protein Form | Heterodimer |
Expression System | Wheat germ |
For Use With (Application) | Flow Cytometry,Immunocytochemistry,Immunofluorescence,Immunohistochemistry,Cell Painting |
Protein Tag | None |
Species | Wheat |
Protein | Fluorescent lectins |
Protein Subtype | Agglutinin |
Thermo Fisher Scientific offers a broad selection of Invitrogen concanavalin A (Con A) conjugates, including Alexa Fluor™ and Alexa Fluor™ Plus conjugates.
Shipping Condition | Room Temperature |
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Ligand Type | Lectin |
Protein Family | Lectins |
Purity or Quality Grade | Imaging Quality |
Form | Lyophilized |
Research Category | Imaging |
Cross Reactivity | α-man, α-glc |
For Use With (Application) | Immunocytochemistry,Immunohistochemistry,Immunofluorescence |
Protein Tag | None |
Species | C. ensiformis |
Source | Canavlia ensiformis |
Recombinant | Native |
The Fluorescein Lectin Screening Kit I provides 1 mg each of the following fluorescein-lectin conjugates: Con A, DBA, PNA, RCA120, SBA, UEA I, WGA. Selected to offer a variety of sugar specificities, each is prepared from our affinity-purified lectins and labeled using the high quality fluorescein isothiocyanate (excitation 495 nm, emission 515 nm) and conjugation procedures that ensure an optimal ratio of fluorophore to lectin. Supplied in solution essentially free of unconjugated fluorophore and preserved with sodium azide.
Sambucus nigra lectin, isolated from elderberry bark, binds preferentially to sialic acid attached to terminal galactose in α-2,6 and to a lesser degree, α-2,3 linkage. Binding is also inhibited to some extent by lactose or galactose. This lectin appears to bind sialic acid linked to N-acetylgalactosamine or galactose. SNA has been reported to inhibit cell-free protein synthesis. 2mg; Solution: 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide, 0.1 mM CaCl2.
Lycopersicon esculentum lectin (LEL or tomato lectin, TL) is a single-subunit glycoprotein containing arabinose and galactose and may form multimeric aggregates in solution. Although it shares specificities with potato lectin, Datura lectin, and wheat germ agglutinin, LEL is dissimilar in many respects. It binds well to glycophorin and Tamm-Horsfall glycoprotein, has been used to label vascular endothelium in rodents, and is an effective marker of blood vessels and microglial cells in rodents. Conjugation with a fluorophore facilitates fast, one-step detection and visualization using intravascular perfusion methods or direct application to tissue sections. This fluorescein-labeled LEL features a ratio of fluorophores to lectin protein that provides optimal staining (excitation 495 nm, emission 515 nm). Supplied as a solution essentially free of unconjugated fluorophores, it is preserved with sodium azide. The recommended inhibiting/eluting sugar is chitin hydrolysate.
Affinity purified Maackia amurensis lectin (MAA) is a tetramer formed by MAA1 or MAL (a 37kD subunit called Maackia amurensis leukoagglutinin) and MAA2 or MAH (a 32 kD subunit called Maackia amurensis hemagglutinin).Technical Specifications:Ligand Source:Maackia amurensisMolecular Weight: 130 kDaCarbohydrate Specificity:Siaα1,3Galβ4GalNAcInhibiting/Eluting Sugar:LactoseCarbohydrate for Elution: 0.2MLactoseBlood Group Specificity: N/AConjugate: FITCForm: LiquidIsoelectric (pI): 4.7Storage: -20°CApplication note: N/A
Griffonia simplicifolia lectin I (GSL I) is a family of glycoproteins with molecular weights of approximately 114 kDa. Two subunits ("A" and "B") have slightly different molecular weights and combine to form tetrameric structures, resulting in five isolectins. The "A"-rich lectin preferentially agglutinates blood group A erythrocytes and appears to be specific for α-N-acetylgalactosamine residues, while the "B"-rich lectin preferentially agglutinates blood group B cells and is specific for α-galactose residues. Our GSL I is a mixture of the five isolectins and binds several glycoproteins, including laminin. This fluorescein-labeled GSL I features a ratio of fluorophores to lectin protein that provides optimal staining (excitation 495 nm, emission 515 nm). Supplied as a solution essentially free of unconjugated fluorophores, it is preserved with sodium azide.
Vicia villosa lectin (VVL, VVA) preferentially binds α- or β-linked terminal N-acetylgalactosamine, especially a single α-N-acetylgalactosamine residue linked to serine or threonine in a polypeptide (the Tn antigen). This lectin may require specific amino acid sequences at the receptor site of glycosylation, and the disaccharide galactosyl (α-1,3) N-acetylgalactosamine is also a potent inhibitor. This conjugate features a ratio of biotin to lectin protein that provides optimal avidin/streptavidin and lectin binding activity. Supplied essentially free of unconjugated biotins, it is preserved with sodium azide.
Arachis Hypogaea (peanut) Lectin- Texas Red:
Sambucus nigra lectin (SNL) is isolated from elderberry bark and binds preferentially to sialic acid attached to terminal galactose in a-2,6 and to a lesser degree, a-2,3 linkage. Also called SNA (Sambucus nigra agglutinin) and EBL (elderberry lectin), SNL can inhibit cell-free protein synthesis. This agarose-bound SNL is prepared using affinity-purified lectin and heat stable, cross-linked 4% agarose beads. The attachment of the lectin through a hydrophilic spacer arm to the beads is carefully controlled to preserve the activity of and minimize conformational changes to the bound lectins. The recommended inhibiting/eluting sugar is 500 mM lactose in buffered saline followed by 500 mM lactose in acetic acid.
NC2683673 PHA-P 2X10 MG
Pure Maackia amurensis lectin (MAA), Texas Red ConjugatedTechnical Specifications:Ligand Source:Maackia amurensisMolecular Weight:130 kDaCarbohydrate Specificity:Siaα1,3Galβ4GalNAcInhibiting/Eluting Sugar:LactoseCarbohydrate for Elution:0.2MLactoseBlood Group Specificity:N/AConjugate: Texas RedForm:LiquidIsoelectric (pI):4.7Storage: -20°CApplication note: N/A
Technical Specifications:Ligand Source:Maackia amurensisMolecular Weight:130 kDaCarbohydrate Specificity:Siaα1,3Galβ4GalNAcInhibiting/Eluting Sugar:LactoseCarbohydrate for Elution:0.2MLactoseBlood Group Specificity:N/AConjugate: RhodamineForm:LiquidIsoelectric (pI):4.7Storage: -20°CApplication note: N/A
Artocarpus integrifolia (Jacalin) Lectin isolated from Artocarpus integrifolia (jackfruit) seeds. Specifications: Primary sugar specificity: Galactose Preferential binding activity: galactosyl (beta-1,4) N-acetylglucosamine.
Sambucus Nigra Lectin - Rhodamine: