Additional Cell Culture Reagents and Supplements
CELLULAR TECHNOLOGY LTD Ctl Anti-Aggregate Wash 20X, Size 1 ml
Ctl Anti-Aggregate Wash 20X, Size 1 Ml. Stabilizes Pbmc During Thawing And Prevents Cell Loss Through Aggregation. Cells That Die During Freeze-Thawing Release Dna Strands That Can Cause Cell Clumping And Aggregation. Therefore, The Use Of Dnase-Containing Wash Solutions Is Recommended While Thawing Pbmc. Moreover, Cells Show Increased Metabolic Activity After Thawing As They Compensate For The Stress Reaction - This Needs To Be Accounted For By Using A Nutrient-Rich Medium. Ctl Anti-Aggregate Wash Supplement Has Been Specifically Formulated For Optimal Pbmc Recovery And Functionality After Thawing. It Contains Injection-Grade Water Supplemented With Amino Acids, Vitamins, Inorganic Salts, And Human Serum Albumin (Hsa) In A Proprietary Composition, Saturated And Unsaturated Fatty Acids, And Sugars In A Ready-To-Use Formulation, Providing A Balanced Environment For Pbmc. For Each Vial Of Pbmc To Be Thawed, 1 Vial Of Ctl Anti-Aggregate Wash Supplement 20X Is Needed.
InVitria ITSE Animal-Free - 10mL
InVitria’s ITS, ITSE, and ITSE+A Animal-Free are recombinant, blood-free cell culture media supplements used to optimize cell growth. For optimal proliferation and performance, cells cultured must be supplied with a critical proteins and components:Insulin: promotes uptake of glucose and amino acids, facilitating cellular signalingTransferrin: delivers intracellular iron via the transferrin receptor, drives cellular respiration, chelates free iron, and reduces generation of cytotoxic free radicals and peroxideSelenium: required for the activity of antioxidant enzymes such as glutathione peroxidase and thioredoxin reductaseEthanolamine: phospholipid precursor that improves the performance of cells in serum-free media and is required for the growth of certain cell typesAlbumin: binds, transports, and delivers ions, fatty acids, vitamins, hormones, small-molecule drugs, and toxins; regulates serum pH and osmotic pressure; and provides antioxidant activityMade in the USA.
STEMCELL Technologies ImmunoCult™ Human CD3/CD28/CD2 T Cell Activator
Achieve robust activation and expansion of T cells in the absence of magnetic beads, feeder cells, or antigens. This product’s gentle activation stimulus ensures a high viability of activated T cells, which can be further expanded in ImmunoCult™-XF T Cell Expansion Medium (Catalog #10981) or other media for culturing human T cells. ImmunoCult™ Human CD3/CD28/CD2 T Cell Activator consists of soluble antibody complexes that bind to and cross-link CD3, CD28, and CD2 cell surface ligands, thereby providing the required primary and co-stimulatory signals for T cell activation. This product is designed for cell therapy research applications, but may be qualified for use as an ancillary material following the framework outlined in USP. STEMCELL can work with you to qualify this reagent as an AM under an approved investigational new drug or clinical trial application.
SIGMA ALDRICH FINE CHEMICALS BIOSCIENCES BURKHOLDERIA CEPACIA SEL SUPP
NC3371068 BURKHOLDERIA CEPACIA SEL SUPP
Sigma Aldrich Fine Chemicals Biosciences Burkholderia cepacia Selective Agar
Burkholderia cepacia Selective Agar | Composition (agar, 15.0 g/L; crystal violet, 0.002 g/L; lactose, 10.000 g/L; phenol red, 0.080 g/L; sodium chloride, 5.0 g/L; sucrose, 10.0 g/L; tryptone, 10.0 g/L; yeast extract, 1.5 g/L), 500g
Advanced Biomatrix Poly-D-Lysine, Solution, 0.1 mg/mL
Poly-D-Lysine comes with 50 mL of a 0.1 mg/ml sterile-filtered solution. Poly-D-Lysine is a synthetic amino acid chain that is positively charged and widely used as a coating to enhance cell attachment and adhesion to both plasticware and glass surfaces. This molecule is resistant to enzymatic degradation and has been used to culture a wide variety of cell types. The molecular weight of Poly-D-Lysine can vary significantly with lower molecular weight (30,000 Da) being less viscous and higher molecular weight (>300,000 Da) having more binding sites per molecule. This product’s molecular weight ranges from 70,000 to 150,000 Da yielding a solution viscosity for easy handling while providing sufficient binding sites for cell attachment. Poly-D-Lysine surface coatings are designed to improve cell attachment, growth and differentiation of many cell types. Coated surfaces will often improve cell attachment in reduced or serum-free conditions.
VITRO BIOPHARMA MSC-GRO™ VitroPlus III Low Serum Complete Medium
VitroPlus III, Low Serum Complete, 500ml
Sigma Aldrich Fine Chemicals Biosciences Hanks’ Balanced Salt Solution 10x, 500 mL
Calcium and magnesium support cell adhesion. Recommended for suspension cultures or whenever cell clumping is a problem.
Cellular Dynamics International iCell Neural Supplement A, 2 mL
2 mL Neuronal medium supplement, serum-free.
Sigma Aldrich Fine Chemicals Biosciences Hoagland′s No. 2 Basal Salt Mixture, 1 L
Used in the preparation of Hoagland′s solution for treating root tips.
Lonza Walkersville RtEGM Retinal Epithelial Cell Growth Medium SingleQuots Supplements and Growth Factors
RtGM SingleQuot Kit are components sold separately and does not include the basal medium. These are part of the RtEGM Retinal Epithelial Cell Growth Medium BulletKit (Catalog Number 195409). The full RtEGM BulletKit consisting of basal medium and supplements can be purchased together using the catalog number 195409.RtEGM Retinal Pigment Epithelial Cell Growth Medium is optimized and performance tested for growing Human RPE cells. It contains 2% serum.Applications:Age-related macular degenerationRetinitis pigmentosaDiabetic retinopathyPhotoreceptor degenerationGene expressionCellular interactionsContent:1 x Bottle with L-Glutamine 4.00 mL, 1 x Gray Cap Vial with hFGF-B 1.00 mL, 1 x Bottle FBS 4.00 mL, 1 x Red Cap Vial with GA-1000 0.20 mL
Cellular Dynamics International iCell Endothelial Cells Medium Supplement, 50 mL
50 mL Endothelial cells medium supplement, serum-based.
CELLULAR TECHNOLOGY LTD CTL-Wash™ Supplement 10x, serum-free formulation
Ctl-Wash Is A Formulation Specially Made To Keep Pbmc In A Nutrient-Rich Environment While Maintaining Their Full Viability And Functionality During Washing. The Two To Three Washing Steps Required After The Ficoll Isolation Or During Thawing Of Pbmc And The Time Required For Their Counting Typically Adds Up To 30-40 Minutes. During This Time, The Pbmc Quality Will Deteriorate If Kept In A Nutritionally-Deficient Environment. If Uncharacterized Serum Is Added, Mitogenic Or Toxic Effects Can Affect The Cells. Ctl-Wash Supplement Is A 10X Serum-Free Formulation Which Has To Be Added To Rpmi-1640. It Contains Injection-Grade Water Supplemented With Amino Acids, Vitamins, Inorganic Salts, And Human Serum Albumin (Hsa) In A Proprietary Composition To Provide A Balanced Environment For Maximizing Pbmc Survival. Ctl-Wash Supplement 10X Is Sold In Convenient Sterile 100Ml Units Ready For Dilution.