Additional Cell Culture Reagents and Supplements
Gibco™ Insulin-Transferrin-Selenium (ITS -G) (100X)
A basal medium supplement to reduce the amount of fetal bovine serum (FBS) needed to culture cells
MilliporeSigma™ Polybrene Infection/Transfection Reagent
A highly efficient method of gene transfer into mammalian cells leveraging infection with retroviral vectors.
MilliporeSigma™ Poly-L-Lysine Solution
Used as coating to enhance cell attachment and adhesion to both plasticware and glass surfaces
Gibco™ CTS™ ACK Lysing Buffer
CTS ACK (Ammonium-Chloride-Potassium) Lysing Buffer is used for the lysis of red blood cells in samples containing white blood cells, such as EDTA-treated whole blood, buffy coats, and bone marrow.
MilliporeSigma™ EmbryoMax™ 0.1% Gelatin Solution
may be used for routine mouse embryonic stem cell culture applications.
MilliporeSigma™ Sodium Butyrate Solution
enhances the transient expression of plasmids containing the SV40 early promoter in transfected cells.
MilliporeSigma™ ESGRO Complete™ Gelatin Solution
For stem cell culture
R&D Systems™ N-2 MAX Media Supplement (100X)
Culture neural progenitor cells and their differentiated derivatives under defined, serum-free conditions
MP Biomedicals™ Complement, Guinea Pig Purified, 5mL
Suitable for use in hemolytic titrations and cytotoxicity assays, and as a serum protein control in immunoelectrophoresis, Ouchterlony immunodiffusion, and other immunoprecipitation techniques
R&D Systems™ N21-MAX Media Supplement (50X)
For long-term culture of neurons under fully defined, serum-free conditions
MilliporeSigma™ EmbryoMax™ 2-Mercaptoethanol (100X)
may be used for routine mouse embryonic stem cell culture applications.