Digitonin, a water-soluble detergent, is used in biological research to permeabilize cell membranes, allowing the study of intracellular components. This 100mg quantity is highly effective for membrane disruption, especially in experiments involving membrane protein isolation or intracellular enzyme activity analysis. Digitonin works by binding to cholesterol in cell membranes, forming pores that allow larger molecules to pass through, making it an ideal tool for cell fractionation and membrane permeability studies. It is commonly used in molecular biology, biochemistry, and cell biology experiments, including the preparation of cell extracts for downstream applications like enzyme assays or protein analysis.