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The Charles River Laboratories KTA-Kinetic Turbidimetric LAL Reagent is an FDA-licensed assay designed for quantitative detection of bacterial endotoxins. This reagent is suitable for both kinetic turbidimetric and gel-clot analyses, ensuring accurate results even in complex samples due to its buffered formulation. It requires a microplate reader and endotoxin-measuring software for operation, which enhances throughput and offers reliable quantitative results.
We are committed to bringing you Greener Alternative Products which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry compared to the standard use of phenol and chloroform to perform DNA extractions.
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The GenElute Endotoxin-free Plasmid Kit offers a simple rapid cost effective method for purifying plasmid DNA with 0.1 EU/mug DNA for high efficiency transfection. Endotoxins (also known as lipopolysaccharides or LPS) are often co-purified with plasmid DNA and significantly reduce transfection efficiencies in endotoxin-sensitive cell lines. Recombinant E. coli is harvested from an overnight culture by centrifugation and subjected to a modified alkaline-SDS lysis procedure to produce a cleared lysate. Endotoxins are removed from the cleared lysate with simple extraction-phase separation steps. Plasmid DNA is further purified by adsorption onto silica in the presence of high salts. After a simple spin-wash step the bound plasmid DNA is eluted in endotoxin-free water. Up to 250 mug of endotoxin-free plasmid DNA may be prepared from 5 to 40 mL of culture.The recovered plasmid DNA is predominately in its supercoiled form. The DNA is ready for immediate use in downstream applications su
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