Immunohistochemistry Reagents

Thermo Scientific™ Pierce™ Methanol-free Formaldehyde Ampules Available on GSA/VA Contract for Federal Government customers only.

Vials of high-quality, methanol-free formaldehyde, a general IHC fixative and reversible amine-crosslinking agent for protein and nucleic acid samples.

Thermo Scientific™ Pierce™ LDH Cytotoxicity Assay Kit Available on GSA/VA Contract for Federal Government customers only.

Quantitatively measure lactose dehydrogenase released into the media from damaged cells as a biomarker for cellular cytotoxicity and cytolysis using a colorimetric assay.

Invitrogen™ eBioscience™ Fixation/Permeabilization Concentrate Non-distribution product as customer accommodation.

Intended to be used with antibodies to transcription factors and nuclear proteins, such as Foxp3 and Ki-67, as well as cytokines and chemokines.

Thermo Scientific™ Pierce™ DAPI Nuclear Counterstain Available on GSA/VA Contract for Federal Government customers only.

Highly pure diamidino-2-phenylindole (DAPI) dye for fluorescent staining of DNA content and nuclei in fixed-cell imaging techniques.

Thermo Scientific™ Pierce™ Hoechst 3342 Fluorescent Stain

High-quality Hoechst dye solution for fluorescent staining of DNA and nuclei in fixed- or live-cell imaging techniques.

BD OptEIA™ Reagent Set B

Sufficient for twenty 96-well plates

BD BV480 Mouse Anti-Human CD45

CD45 is encoded by the PTPRC (Protein tyrosine phosphatase receptor type C) gene. BD Horizon™ BV480 Mouse Anti-Human CD45 is present on all human leukocytes including lymphocytes, monocytes, granulocytes, eosinophils, and thymocytes. CD45 is absent from circulating erythrocytes, platelets, or mature erythroid cells of bone marrow and non-hemopoietic tissues.

Thermo Scientific™ Pierce™ Protein Transfection Reagent Available on GSA/VA Contract for Federal Government customers only.

Form complexes with proteins, peptides, antibodies and other biologically active molecules for direct intracellular delivery using this cationic lipid mixture.

Meridian Bioscience™ Latex Agglutination System Available on GSA/VA Contract for Federal Government customers only.

Qualitative and semi-quantitative latex test for detection of Cryptococcus neoformans

Thermo Scientific™ Pierce™ Metal Enhanced DAB Substrate Kit Available on GSA/VA Contract for Federal Government customers only.

Special formulation of diaminobenzidine peroxidase substrate that uses cobalt and nickel chloride to yield intense color for IHC staining.

Novex™ Endogenous Biotin-Blocking Kit Available on GSA/VA Contract for Federal Government customers only. Non-distribution product as customer accommodation.

Endogenous Biotin-Blocking Kit

Thermo Scientific™ Pierce™ ABC Staining Kits Available on GSA/VA Contract for Federal Government customers only.

Detect biotinylated secondary antibodies by IHC using the highly sensitive avidin-biotin complex (ABC) technique.

BD BV480 Mouse Anti-Human CD8

CD8 is expressed by majority of thymocytes, by subpopulations of αβ T cells and γδ T cells and by some NK cells. BD Horizon™ BV480 Mouse Anti-Human CD8 plays important roles in T cell activation and selection.

BD BV480 Mouse Anti-Human CD28

The CD28.2 monoclonal antibody specifically binds to CD28, a 44kDa homodimeric transmembrane glycoprotein present on most mature T cells, thymocytes and plasma cells. BD Horizon™ BV480 Mouse Anti-Human CD28 is a costimulatory receptor that binds CD80 and CD86 as ligands and plays a very important role in T cell-B cell interactions.

BD BV480 Rat Anti-Mouse CD45

BD Horizon - BV480 Rat Anti-Mouse CD45 - CD45 is a transmembrane glycoprotein which is expressed at high levels on cell-surface, and its presence distinguishes leukocytes from non-hematopoietic cells. CD45 isoforms play complex roles in T-cell and B-cell antigen receptor signal transduction and the CD45 isoforms detected in the mouse are cell type-, maturation-, and activation state-specific.

MilliporeSigma™ Black Gold II Stain

Stains specifically for myelin within the central nervous system, provides high resolution, high contrast, a short histochemical processing time, and high reproducibility.

Molecular Probes™ Human Neural Stem Cell Immunocytochemistry Kit Available on GSA/VA Contract for Federal Government customers only. Non-distribution product as customer accommodation.

Human Neural Stem Cell Immunocytochemistry Kit

Invitrogen™ eBioscience™ IHC Antigen Retrieval Solution, Low pH, 10x Non-distribution product as customer accommodation.

Designed for use during the heat induced epitope retrieval (HIER) step prior to immunohistochemistry on formalin-fixed paraffin-embedded tissue sections. Used in combination with heat has been shown to restore the antigenicity of proteins modified during the formalin fixation of tissue.

Invitrogen™ Click-iT™ TUNEL Colorimetric IHC Detection Kit Non-distribution product as customer accommodation.

Click-iT TUNEL Colorimetric IHC Detection Kit

Invitrogen™ eBioscience™ IHC Antigen Retrieval Solution, High pH, 10x Non-distribution product as customer accommodation.

Designed for use during the heat induced epitope retrieval (HIER) step prior to immunohistochemistry on formalin-fixed paraffin embedded tissue sections. Use in combination with heat has been shown to restore the antigenicity of proteins modified during the formalin fixation of tissue.

BD BV480 Mouse Anti-Human CD3

CD3ε is expressed on 70-80% of normal human peripheral blood lymphocytes and 60-85% of thymocytes. BD Horizon™ BV480 Mouse Anti-Human CD3 plays a central role in signal transduction during antigen recognition. The UCHT1 antibody stains both surface and intracellular CD3ε unlike the other CD3 clone, HIT3a, that stains only extracellular CD3ε

Molecular Probes™ Pluripotent Stem Cell 4-Marker Immunocytochemistry Kit Available on GSA/VA Contract for Federal Government customers only. Non-distribution product as customer accommodation.

Pluripotent Stem Cell 4-Marker Immunocytochemistry Kit

BD BV480 Rat Anti-Human CXCR5 (CD185)

In peripheral blood, CXCR5 expression is restricted to B lymphocytes and a small subset of CD4+ and CD8+ lymphocytes. BD Horizon™ BV480 Rat Anti-Human CXCR5 (CD185) has restricted expression pattern of CXCR5 on B cells and follicular T helper cells (Tfh) that suggests this receptor functions as a regulator of B and T cell migration.

Invitrogen™ eBioscience™ TBS Wash Buffer for IHC/ICC, 20x Non-distribution product as customer accommodation.

For use in immunohistochemistry and immunocytochemistry protocols which require washing to remove non-specific binding. TBS Wash Buffer for IHC/ICC is recommended for eFluor™ Nanocrystal conjugated-antibodies following antibody incubation.

BD BV480 Mouse Anti-Human CD279 (PD-1)

CD279 is an immunoregulatory receptor expressed on activated T cells, B cells, and myeloid cells. BD Horizon™ BV480 Mouse Anti-Human CD279 (PD-1) contains an immunoreceptor tyrosine-based inhibitory motif (ITIM) in the cytoplasmic region. Mice deficient in CD279 show a breakdown of peripheral tolerance and manifest multiple autoimmune symptoms.

BD Hoechst 33342 Solution

Bioimaging certified reagent for fluorescent staining of DNA and nuclei in live or fixed cells

BD Reagent Set A

Use in BD OptEIA™ ELISA Sets

Invitrogen™ eBioscience™ BrdU Kit for IHC/ICC Immunofluorescence eFluor™ 570 Non-distribution product as customer accommodation.

Contains reagents and buffers to identify and examine proliferating cells by immunohistochemical or immunocytochemical analysis. Optimized for IHC with both frozen and formalin-fixed paraffin-embedded BrdU-labeled mouse intestine and ICC of BrdU-pulsed HeLa cells grown on culture slides.

BD BV480 Mouse Anti-Human CD19

CD19 is expressed throughout B-lymphocyte development from the pro-B cell through the mature B-cell stages. BD Horizon™ BV480 Mouse Anti-Human CD19 participates in B-lymphocyte development, B-cell activation, maturation of memory B cells and regulation of tolerance. CD19 has also been detected on peritoneal mast cells, co-localized with CD21/CD35, and it is proposed to play a role in complement-mediated mast-cell activation.

BD BV480 Rat Anti-Human CD197 (CCR7)

CCR7 mRNA is expressed mainly in lymphoid tissues including spleen, lymph nodes and tonsil. BD Horizon™ BV480 Rat Anti-Human CD197 (CCR7) is also detected in peripheral T and B lymphocytes, in bone marrow and cord blood CD34-positive cells and mature dendritic cells.

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