Immunohistochemistry Reagents

Thermo Scientific™ Pierce™ 16% Formaldehyde (w/v), Methanol-free Available on GSA/VA Contract for Federal Government customers only.

Vials of high-quality, methanol-free formaldehyde, a general IHC fixative and reversible amine-crosslinking agent for protein and nucleic acid samples.

Thermo Scientific™ DAPI Solution (1 mg/mL) Available on GSA/VA Contract for Federal Government customers only.

Highly pure diamidino-2-phenylindole (DAPI) dye for fluorescent staining of DNA content and nuclei in fixed-cell imaging techniques.

Invitrogen™ eBioscience™ Fixation/Permeabilization Concentrate Non-distribution product as customer accommodation.

Intended to be used with antibodies to transcription factors and nuclear proteins, such as Foxp3 and Ki-67, as well as cytokines and chemokines.

BD BV480 Mouse Anti-Human CD45

CD45 is encoded by the PTPRC (Protein tyrosine phosphatase receptor type C) gene. BD Horizon™ BV480 Mouse Anti-Human CD45 is present on all human leukocytes including lymphocytes, monocytes, granulocytes, eosinophils, and thymocytes. CD45 is absent from circulating erythrocytes, platelets, or mature erythroid cells of bone marrow and non-hemopoietic tissues.

Thermo Scientific™ Hoechst 33342 Solution (20 mM)

High-quality Hoechst dye solution for fluorescent staining of DNA and nuclei in fixed- or live-cell imaging techniques.

BD OptEIA™ Reagent Set B

Sufficient for twenty 96-well plates

Thermo Scientific™ Metal Enhanced DAB Substrate Kit Available on GSA/VA Contract for Federal Government customers only.

Special formulation of diaminobenzidine peroxidase substrate that uses cobalt and nickel chloride to yield intense color for IHC staining.

BD BV480 Rat Anti-Human CD197 (CCR7)

CCR7 mRNA is expressed mainly in lymphoid tissues including spleen, lymph nodes and tonsil. BD Horizon™ BV480 Rat Anti-Human CD197 (CCR7) is also detected in peripheral T and B lymphocytes, in bone marrow and cord blood CD34-positive cells and mature dendritic cells.

Thermo Scientific™ ABC Peroxidase Standard Staining Kit

Detect biotinylated secondary antibodies by IHC using the highly sensitive avidin-biotin complex (ABC) technique.

BD BV480 Mouse Anti-Human CD4

CD4 distribution on lymphocytes is similar for both human and monkey cells. BD Horizon™ BV480 Mouse Anti-Human CD4,  with the majority of CD4-positive lymphocytes being CD8-negative and lacking reactivity with antibodies to B- or NK-cell markers.

Invitrogen™ eBioscience™ IHC Antigen Retrieval Solution - High pH (10X) Non-distribution product as customer accommodation.

Designed for use during the heat induced epitope retrieval (HIER) step prior to immunohistochemistry on formalin-fixed paraffin embedded tissue sections. Use in combination with heat has been shown to restore the antigenicity of proteins modified during the formalin fixation of tissue.

BD BV480 Mouse Anti-Human CD3

CD3ε is expressed on 70-80% of normal human peripheral blood lymphocytes and 60-85% of thymocytes. BD Horizon™ BV480 Mouse Anti-Human CD3 plays a central role in signal transduction during antigen recognition. The UCHT1 antibody stains both surface and intracellular CD3ε unlike the other CD3 clone, HIT3a, that stains only extracellular CD3ε

Thermo Scientific™ Pierce™ Protein Transfection Reagent Available on GSA/VA Contract for Federal Government customers only.

Form complexes with proteins, peptides, antibodies and other biologically active molecules for direct intracellular delivery using this cationic lipid mixture.

Thermo Scientific™ Pierce™ LDH Cytotoxicity Assay Kit

Quantitatively measure lactose dehydrogenase released into the media from damaged cells as a biomarker for cellular cytotoxicity and cytolysis using a colorimetric assay.

Invitrogen™ eBioscience™ IHC Antigen Retrieval Solution - Low pH (10X) Non-distribution product as customer accommodation.

Designed for use during the heat induced epitope retrieval (HIER) step prior to immunohistochemistry on formalin-fixed paraffin-embedded tissue sections. Used in combination with heat has been shown to restore the antigenicity of proteins modified during the formalin fixation of tissue.

Meridian Bioscience™ Latex Agglutination System Available on GSA/VA Contract for Federal Government customers only.

Qualitative and semi-quantitative latex test for detection of Cryptococcus neoformans

Invitrogen™ Click-iT™ TUNEL Colorimetric IHC Detection Kit Non-distribution product as customer accommodation.

Click-iT TUNEL Colorimetric IHC Detection Kit

Molecular Probes™ Pluripotent Stem Cell 4-Marker Immunocytochemistry Kit Available on GSA/VA Contract for Federal Government customers only. Non-distribution product as customer accommodation.

Pluripotent Stem Cell 4-Marker Immunocytochemistry Kit

BD BV480 Mouse Anti-Human CD4

CD4 is expressed on T-helper/inducer lymphocytes and monocytes. BD Horizon™ BV480 Mouse Anti-Human CD4 serves as a T cell coreceptor for MHC class II antigen recognition and as a receptor for the human immunodeficiency virus (HIV). The SK3 antibody inhibits HIV binding to CD4+ cells.

BD BV480 Mouse Anti-Human IgD

mIgD is expressed on mature naive B cells (along with membrane IgM) and serves as a B-cell receptor for antigen (BCR). BD Horizon™ BV480 Mouse Anti-Human IgD BCR, in association with other signaling molecules including CD79a and CD79b, can transduce activating or tolerizing signals intracellularly into B lymphocytes.

BD BV480 Mouse Anti-Human IFN- γ

IFN-γ is a potent multifunctional cytokine that is produced by several activated cell types. BD Horizon™ BV480 Mouse Anti-Human IFN- γ can exert strong regulatory influences on the proliferation, differentiation, and effector responses of B cell and T cell subsets.

Molecular Probes™ Human Neural Stem Cell Immunocytochemistry Kit Available on GSA/VA Contract for Federal Government customers only. Non-distribution product as customer accommodation.

Human Neural Stem Cell Immunocytochemistry Kit

BD BV480 Rat Anti-Mouse CD11b

CD11b serves as the alpha chain of the heterodimeric Mac-1 integrin (CD11b/CD18, αMβ2), also known as complement receptor 3 (CR3). BD Horizon™ BV480 Rat Anti-Mouse CD11b is a 170-kDa type 1 transmembrane glycoprotein and belongs to the Integrin alpha chain family.

Novex™ Endogenous Biotin-Blocking Kit Available on GSA/VA Contract for Federal Government customers only. Non-distribution product as customer accommodation.

Endogenous Biotin-Blocking Kit

BD BV480 Mouse Anti-Human CD279 (PD-1)

CD279 is an immunoregulatory receptor expressed on activated T cells, B cells, and myeloid cells. BD Horizon™ BV480 Mouse Anti-Human CD279 (PD-1) contains an immunoreceptor tyrosine-based inhibitory motif (ITIM) in the cytoplasmic region. Mice deficient in CD279 show a breakdown of peripheral tolerance and manifest multiple autoimmune symptoms.

BD BV480 Mouse Anti-Human CD1a

CD1a is expressed on cortical thymocytes, dendritic cells and Langerhans cells. BD Horizon™ BV480 Mouse Anti-Human CD1a has structural similarities to the MHC class I antigen, and plays a role in antigen presentation. 

Thermo Scientific™ Ultra-Sensitive ABC Peroxidase Standard Staining Kit

Detect biotinylated secondary antibodies by IHC using the highly sensitive avidin-biotin complex (ABC) technique.

Thermo Scientific™ DAPI (4',6-diamidino-2-phenylindole, dihydrochloride)

Highly pure diamidino-2-phenylindole (DAPI) dye for fluorescent staining of DNA content and nuclei in fixed-cell imaging techniques.

BD Reagent Set A

Use in BD OptEIA™ ELISA Sets

BD BV480 Rat Anti-Mouse CD45

BD Horizon - BV480 Rat Anti-Mouse CD45 - CD45 is a transmembrane glycoprotein which is expressed at high levels on cell-surface, and its presence distinguishes leukocytes from non-hematopoietic cells. CD45 isoforms play complex roles in T-cell and B-cell antigen receptor signal transduction and the CD45 isoforms detected in the mouse are cell type-, maturation-, and activation state-specific.

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