Miscellaneous Molecular Reagents

Thermo Scientific™ IPTG Available on GSA/VA Contract for Federal Government customers only.

Induce the expression of genes under the control of lac operon in cloning and protein expression procedures.

Thermo Scientific™ Pierce™ Amine-Reactive Tandem Mass Tag Reagents Available on GSA/VA Contract for Federal Government customers only.

For multiplex quantitative protein expression analysis by mass spectrometry

Thermo Scientific™ Pierce™ LTQ Velos ESI Positive Ion Calibration Solution Available on GSA/VA Contract for Federal Government customers only.

Calibrate LTQ Velos, LTQ Orbitrap and other mass spectrometers using a mixture of highly purified ionizable molecules designed for positive mode calibration.

Thermo Scientific™ Pierce™ BS3-d0 and BS3-d4 Crosslinker Mass Pair

Crosslink amines to amines using these heavy (deuterated) and light matched pairs of an 11.4A, NHS-ester crosslinker for mass spectrometry analysis of protein interactions.

Thermo Scientific™ RPMI 1640 Media for SILAC

Analyze protein expression by mass spectrometry using these media or additives for stable isotope labeling with amino acids in cell culture (SILAC).

Thermo Scientific™ X-Gal Solution, ready-to-use Available on GSA/VA Contract for Federal Government customers only.

Effectively screen colonies with X-Gal, an inert chromogenic substrate for beta-galactosidase which hydrolyzes X-Gal, forming an intense blue precipitate.

Thermo Scientific™ Pierce™ Amino Acids for SILAC Labeling Available on GSA/VA Contract for Federal Government customers only.

Study protein expression by mass spectrometry with mass variants of lysine, arginine and other amino acids to perform stable isotope labeling with amino acids in cell culture.

Thermo Scientific™ Pierce™ Amine-Reactive Tandem Mass Tag Reagents Available on GSA/VA Contract for Federal Government customers only.

Study protein expression by quantitative mass spectrometry using amine-labeling compounds of equal total mass (isobaric) but different reporter ions.

Thermo Scientific™ Pierce™ TNBSA (Trinitrobenzene Sulfonic Acid)

Label primary amines (peptides or amino acids) for colorimetric detection (Amax 335nm) using this 5% solution of trinitrobenzene sulfonic acid in methanol.

Thermo Scientific™ DTT Available on GSA/VA Contract for Federal Government customers only.

Stabilize enzymes and other proteins which possess free sulfhydryl groups with DTT, shown to restore activity lost by oxidation of these groups in vitro.

Thermo Scientific™ Pierce™ HeLa Protein Digest Standard Available on GSA/VA Contract for Federal Government customers only.

Use this standard, a highly validated mammalian protein digest, as a quality control sample for mass spectrometry (MS) analysis of complex proteomic samples.

Thermo Scientific™ Pierce™ Fluoraldehyde (OPA) Reagent Available on GSA/VA Contract for Federal Government customers only.

Ready-to-use solution of o-phthalaldehyde, a fluorescent, amine-reactive, HPLC pre- or post-column labeling reagent to measure peptides or amino acids (Ex 340nm, Em 455nm).

Thermo Scientific™ DMEM Media for SILAC

Analyze protein expression by mass spectrometry using these media or additives for stable isotope labeling with amino acids in cell culture (SILAC).

Thermo Scientific™ Pierce™ Hydrochloric Acid Solution

Constant-boiling 6N HCl solution, supplied in 1mL ampules, used to acid-hydrolyze proteins (protein hydrolysis) for HPLC amino acid composition analysis.

Thermo Scientific™ Pierce™ ESI Negative Ion Calibration Solution Available on GSA/VA Contract for Federal Government customers only.

Calibrate LTQ mass spectrometer instruments using a mixture of highly purified ionizable molecules designed for negative mode calibration.

Thermo Scientific™ Pierce™ MS Grade Trypsin Protease Available on GSA/VA Contract for Federal Government customers only.

Ensure maximum activity and stability in proteomic applications with this chemically modified, highly purified porcine trypsin.

Thermo Scientific™ Pierce™ DTNB (Ellman's Reagent)

Measure peptide cysteines or other free, reduced sulfhydryls (-SH groups) in solution using this thiol-reactive, colored compound (Amax 412nm).

Thermo Scientific™ Immobilized Anti-TMT™ Antibody Resin

Thermo Scientific™ Pierce™ Anti-TMT Antibody Resin enables selective enrichment of peptides in complex samples that have been labeled with Tandem Mass Tag™ Reagents.

Thermo Scientific™ Pierce™ C-18 Spin Columns

Desalt, clean up and prep 25 to 150 microliter peptide samples for mass spectrometry with these ready-to-use microcentrifuge columns of C18 reverse-phase resin.

Thermo Scientific™ Pierce™ Cysteine-Reactive Tandem Mass Tag Reagents Available on GSA/VA Contract for Federal Government customers only.

Perform measurement of protein and peptide cysteine modifications (S-nitrosylation, oxidation and disulfide bridges) by multiplex quantitative mass spectrometry.

Thermo Scientific™ Pierce™ Amino Acid Standard H

Calibrate HPLC analysis of protein hydrolysates using this calibration standard mixture of 18 amino acids, each at 2.5 micromoles/mL in 0.1N HCl.

Thermo Scientific™ Pierce™ Quantitative Colorimetric Peptide Assay Kit Available on GSA/VA Contract for Federal Government customers only.

Measure total peptide concentration by a colorimetic microplate method that provides improved sensitivity, reproducibility and ease using the Thermo Scientific™ Pierce™ Quantitative Colorimetric Peptide Assay.

BD Horizon™ BV421 Annexin V

A sensitive probe for identifying apoptotic cells, binding to negatively charged phospholipid surfaces with a higher affinity for phosphatidylserine (PS) than most other phospholipids

Thermo Scientific™ SILAC Protein Quantitation Kit (LysC) - DMEM

Analyze protein expression by mass spectrometry using these media or additives for stable isotope labeling with amino acids in cell culture (SILAC).

Thermo Scientific™ Pierce™ FDAA (Marfey's Reagent)

Label and measure optical isomers of amino acids by reverse-phase chromatography (HPLC) with this amine-reactive, colored (Amax 340nm) compound.

Thermo Scientific™ High-Select™ Phosphopeptide Enrichment Kit

Enriches phosphopeptides from complex samples.

Thermo Scientific™ DMEM:F-12 Media for SILAC

Analyze protein expression by mass spectrometry using these media or additives for stable isotope labeling with amino acids in cell culture (SILAC).

Thermo Scientific™ Pierce™ High pH Reversed-Phase Peptide Fractionation Kit

Increases protein identification from LC/MS analysis through orthogonal peptide fractionation of complex peptide samples using Thermo Scientific™ Pierce™ High pH Reversed-Phase Peptide Fractionation Kit.

Thermo Scientific™ Pierce™ LTQ ESI Positive Ion Calibration Solution

Calibrate LTQ, LTQ Orbitrap, LXQ and LCQ FLEET mass spectrometers using a mixture of highly purified ionizable molecules designed for positive mode calibration.

R&D Systems™ Resazurin Available on GSA/VA Contract for Federal Government customers only.

Simple and quantitative method for measuring cell proliferation, viability and cytotoxicity

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