Thermo Scientific™ 1-Step™ TMB-Blotting Substrate Solution

Description

Ready-to-use blue TMB peroxidase (HRP) substrate specially formulated for chromogenic detection in Western blot and IHC experiments.

Thermo Scientific™ Pierce TMB-Blotting 1-Step Solution is a ready-to-use solution of the blue TMB peroxidase (HRP) substrate that is specially formulated for chromogenic detection in Western blotting and immunohistochemistry methods.

TMB (3,3',5,5'-tetramethylbenzidine; MW = 240.4) is most often used as a soluble substrate to detect the activity of peroxidase-conjugated antibodies in ELISA. Pierce TMB-Blotting Solution is formulated to precipitate and localize the blue reaction product onto membrane surfaces or tissue samples at sites where the peroxidase-substrate reaction occurs. TMB-Blotting Substrate produces a very large signal-to-noise ratio, although its high sensitivity requires careful washing and blocking to avoid background staining. When performing immunohistochemical techniques, aqueous counterstains and mounting medium must be used.

Highlights:

• HRP substrate – for sensitive detection of horseradish peroxidase activity on solid media

• Chromogenic – no special equipment needed for visualization; produces dark blue bands in Western blots; is not as susceptible to fading as AEC or 4-CN substrates

• Ready-to-use – no organic solvents are required to dissolve; no dilution necessary for use

• Complete – does not require other buffers or addition of hydrogen peroxide

Specifications

• Product Type: Substrate
• Detection Method: Colorimetric
• Form: Liquid
• Product Line: 1-Step™
• Quantity: 250 mL
• Substrate: Horseradish Peroxidase
• Reactivity: Horseradish Peroxidase
• Content And Storage: Upon receipt store product at 4°C. Product is shipped at ambient temperature.

For Research Use Only. Not for use in diagnostic procedures.