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ProSci 14 day old Mouse Liver Lysate

Description
- Liver tissue lysate (14 Days Old) was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin
- Tissue and cell debris was removed by centrifugation
- Protein concentration was determined with Bio-Rad protein assay
- The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.
Specifications
Specifications
| Tissue | Liver |
| Physical Form | Frozen |
| Donor Source | Single Donor |
| Gender | Unavailable |
| Quantity | 0.1 mg |
| Tissue Condition | Normal |
| Host Species | Mouse |
| Concentration | 2 mg/mL |
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