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ATCC 209/MDCT; Distal Convoluted Tubule; Mouse (Mus musculus)
Supplier: ATCC CRL3250
209/MDCT is a distal convoluted tubule cell line that was isolated in 1998 from the kidney of a 44-to-55-day-old, male mouse. This continous line of distal convoluted tubule (DCT) cells were immortalized with Adenovirus 12 - SV40 virus hybrid (Ad12-SV40). They express a phenotype including NaCl cotransporter (Slc12a3) and PTH receptors. The DCT is a primary site for regulation of blood pressure by controlling NaCl absorption and for calcium homeostasis by parathyroid hormone. This cell line is of considerable use for studies of hormonal and pharmacological regulation of fundamental ion transport processes and their underlying mechanisms. This cell line was deposited by Friedman, PA.
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