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MP Biomedicals™ 3,3',5,5'-Tetramethylbenzidine Liquid Substrate

3,3',5,5'-Tetramethylbenzide Liquid Substrate is a chromogenic substrate for Horse Radish Peroxidase (HRP). TMB Solution is a convenient, non toxic and ready to use stable chromogen solution suitable for peroxidase and pseudo-peroxidase reactions in ELISA, it provides consistent performance and sensitivity to deliver reproducible results.

Supplier:  MP Biomedicals™ 0215234680

Catalog No. MP215234680

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  • Ready to use 1.25 mmol/L stablized chromogen solution
  • Metabolism of the TMB substrate by HRP creates a soluble, bluish reaction product that may be quantified by measuring absorbance at 370 or 665 nm.
  • Reaction can be stopped by addition of 1 volume of 1N hydrocloric or sulfurc acid to yield a yellow reaction product that can be measured by absorbance at 450 nm (within one hour).


Liquid Substrate
100 mL
4°C, protect from light


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Unless specified otherwise, for research or further manufacturing use only, not for direct human use.