Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More
  1. Home
  2. Products
  3. Chemicals
  4. Organic compounds
  5. Organoheterocyclic compounds
  6. Azoles
  7. Thiazoles
  8. MP021022272

3-(4,5-Dimethylthiazolyl-2)-2,5-Diphenyl Tetrazolium Bromide, MP Biomedicals™

Catalog No. MP021022272 Shop All MP Biomedicals, Inc Products
Change view
Click to view available options
Quantity:
250 mg
1 product options available for selection
Product selection table with 1 available options. Use arrow keys to navigate and Enter or Space to select.
Catalog No. Quantity
MP021022272 250 mg
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 1 options available.
1 options
Catalog No. MP021022272 Supplier MP Biomedicals, Inc Supplier No. 02102227.2
Only null left
Add to Cart
Add to Cart

Description

Used for measurement of cell division and proliferation. 3-(4,5-Dimethylthiazolyl-2)-2,5-Diphenyl Tetrazolium Bromide, MP Biomedicals (MTT) is a histochemical/cytochemical reagent. It is converted to water-insoluble MTT-formazan of a dark blue color by mi

Thiazolyl Blue Tetrazolium Blue (MTT) may be used in measurement of cell proliferation. It has been used as a histochemical/cytochemical reagent and for the detection of NAD. ADP-linked enzyme systems in tissue cannot be detected with MTT, due to binding of the cation by the cyanide trap used.

  • MTT produces a yellowish solution that is converted to dark blue, water-insoluble MTT formazan by mitochondrial dehydrogenases of living cells
  • The blue crystals are solubilized with acidified isopropanol and the intensity is measured colorimetrically at 570nm
  • MTT is rapidly reduced to the formazan, which chelates with nickel, copper, and cobalt; the cobalt chelate has been used in oxidative systems
TRUSTED_SUSTAINABILITY

Chemical Identifiers

CAS 298-93-1
Molecular Formula C18H16BrN5S
Molecular Weight (g/mol) 414.33
MDL Number MFCD00011964,MFCD00066662
InChI Key AZKSAVLVSZKNRD-UHFFFAOYSA-M
Synonym thiazolyl blue, thiazolyl blue tetrazolium bromide, mmt tetrazolium, methylthiazoletetrazolium, mtt, thiazolyl blue monotetrazolium, thiazole blue, 3-4,5-dimethylthiazol-2-yl-2,5-diphenyltetrazolium bromide, mtt van, unii-euy85h477i
PubChem CID 64965
ChEBI CHEBI:53233
IUPAC Name 2-(4,5-dimethyl-1,3-thiazol-2-yl)-3,5-diphenyl-3H-1,2λ⁵,3,4-tetrazol-2-ylium bromide
SMILES [Br-].CC1=C(C)N=C(S1)[N+]1=NC(=NN1C1=CC=CC=C1)C1=CC=CC=C1

Specifications

Absorbance 376 ± 2nm; 242 ± 2nm
Melting Point 189°C to 199°C
Quantity 250 mg
Solubility Information Soluble in methyl alcohol (heat), methanol (10mg/mL with heat, clear, yellow solution) and water (5mg/mL, clear to very slightly hazy, yellow to bright yellow solution).
Formula Weight 414.3
Percent Purity ∼98%
Physical Form Powder

Safety and Handling

Recommended Storage +4°C, protect from light
Product Title
Select an issue

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.