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3-(4,5-Dimethylthiazolyl-2)-2,5-Diphenyl Tetrazolium Bromide, MP Biomedicals™

Catalog Number MP021022272
Click to view available options
Quantity:
250 mg
298-93-1
C18H16BrN5S
414.33
MFCD00011964,MFCD00066662
AZKSAVLVSZKNRD-UHFFFAOYSA-M
thiazolyl blue, thiazolyl blue tetrazolium bromide, mmt tetrazolium, methylthiazoletetrazolium, mtt, thiazolyl blue monotetrazolium, thiazole blue, 3-4,5-dimethylthiazol-2-yl-2,5-diphenyltetrazolium bromide, mtt van, unii-euy85h477i
64965
CHEBI:53233
2-(4,5-dimethyl-1,3-thiazol-2-yl)-3,5-diphenyl-3H-1,2λ⁵,3,4-tetrazol-2-ylium bromide
[Br-].CC1=C(C)N=C(S1)[N+]1=NC(=NN1C1=CC=CC=C1)C1=CC=CC=C1
298-93-1
C18H16BrN5S
414.33
MFCD00011964,MFCD00066662
AZKSAVLVSZKNRD-UHFFFAOYSA-M
thiazolyl blue, thiazolyl blue tetrazolium bromide, mmt tetrazolium, methylthiazoletetrazolium, mtt, thiazolyl blue monotetrazolium, thiazole blue, 3-4,5-dimethylthiazol-2-yl-2,5-diphenyltetrazolium bromide, mtt van, unii-euy85h477i
64965
CHEBI:53233
2-(4,5-dimethyl-1,3-thiazol-2-yl)-3,5-diphenyl-3H-1,2λ⁵,3,4-tetrazol-2-ylium bromide
[Br-].CC1=C(C)N=C(S1)[N+]1=NC(=NN1C1=CC=CC=C1)C1=CC=CC=C1

Description

Used for measurement of cell division and proliferation. 3-(4,5-Dimethylthiazolyl-2)-2,5-Diphenyl Tetrazolium Bromide, MP Biomedicals (MTT) is a histochemical/cytochemical reagent. It is converted to water-insoluble MTT-formazan of a dark blue color by mi

Thiazolyl Blue Tetrazolium Blue (MTT) may be used in measurement of cell proliferation. It has been used as a histochemical/cytochemical reagent and for the detection of NAD. ADP-linked enzyme systems in tissue cannot be detected with MTT, due to binding of the cation by the cyanide trap used.

  • MTT produces a yellowish solution that is converted to dark blue, water-insoluble MTT formazan by mitochondrial dehydrogenases of living cells
  • The blue crystals are solubilized with acidified isopropanol and the intensity is measured colorimetrically at 570nm
  • MTT is rapidly reduced to the formazan, which chelates with nickel, copper, and cobalt; the cobalt chelate has been used in oxidative systems
Absorbance 376 ± 2nm; 242 ± 2nm
Melting Point 189°C to 199°C
Quantity 250 mg
Solubility Information Soluble in methyl alcohol (heat), methanol (10mg/mL with heat, clear, yellow solution) and water (5mg/mL, clear to very slightly hazy, yellow to bright yellow solution).
Formula Weight 414.3
Percent Purity ∼98%
Physical Form Powder

Safety and Handling

Recommended Storage +4°C, protect from light
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