Sigma Aldrich Fine Chemicals Biosciences 5 Bromo 2 deoxy uridine La

Supplier:  Sigma Aldrich Fine Chemicals Biosciences 11296736001

The kit is used for the detection of BrdU incorporated into cellular DNA using immunofluorescence microscopy. It is used for the detection of DNA synthesis by either in vitro labeling of cells or organ cultures or by in vivo labeling in which frozen or paraffin-embedded tissue sections must be prepared prior to fixation.Normally binding of the antibody is only achieved by denaturation of the DNA. This is usually obtained by exposing the cells to acid base or heat. These procedures result in destruction of cell integrity including cell morphology and surface and cytoplasmatic markers.The BrdU Labeling and Detection Kit I avoids these problems. The antibody preparation contains specific nucleases which allows access to BrdU after fixation in acidic ethanol. Therefore also simultaneous detection of other markers (double staining) is possible.

Catalog No. 50-290-6071