X-Gal, 5-Bromo-4-chloro-3-indolyl-β-D-galactopyranoside, Fisher BioReagents™

Description

For blue/white colony screening, immunoblotting and immunocytochemical assays

• Chromogenic substrate used for blue-white selection of recombinant bacterial colonies with the lac+ genotype
• Forms blue precipitate when hydrolyzed by β-galactosidase
• Soluble in DMF and Methanol

Specifications

Chemical Identifiers

• CAS: 7240-90-6
• Molecular Formula: C14H15BrClNO6
• Molecular Weight (g/mol): 408.629
• InChI Key: OPIFSICVWOWJMJ-AEOCFKNESA-N
• Synonym: x-gal, 5-bromo-4-chloro-3-beta-d-galactopyranosyloxy indole, 5-bromo-4-chloro-3-indolyl-beta-d-galactoside, unii-v595og374w, 5-bromo-4-chloro-3-indolyl beta-galactoside, 5-bromo-4-chloroindol-3-yl-beta-d-galactopyranoside, 5-bromo-4-chloro-3-indolyl beta-d-galactoside, 5-bromo-4-chloro-3-indolyl-beta-d-galactopyranoside, 5-bromo-4-chloro-3-indolyl beta-d-galactopyranoside, indoxyl-gal
• PubChem CID: 65181
• ChEBI: CHEBI:75055
• IUPAC Name: (2S,3R,4S,5R,6R)-2-[(5-bromo-4-chloro-1H-indol-3-yl)oxy]-6-(hydroxymethyl)oxane-3,4,5-triol
• SMILES: C1=CC(=C(C2=C1NC=C2OC3C(C(C(C(O3)CO)O)O)O)Cl)Br

• Alpha Vector: Bromo-4-chloro-3-indolyl-[beta]-D-galactopyranoside (X-Gal)
• Melting Point: 230°C
• Color: White
• Packaging: Glass Bottle
• Quantity: 100 mg
• Merck Index: 15, 10272
• Specific Rotation: -56 to -64° (+ or -)
• Formula Weight: 408.63
• Physical Form: Powder or Solid
• Chemical Name or Material: 5-Bromo-4-chloro-3-indolyl- β-D-galactopyranoside

Safety and Handling

Recommended Storage

-20°C

SDS