Cytiva 5 mL HiTrap™ MabSelect™ SuRe 70 Column

Description

Protein A resin with high binding capacity for cost-efficient separation mAbs. Column for antibody affinity chromatography.

MabSelect™ SuRe 70 protein A resin with high dynamic binding capacity (DBC), enables highly cost-efficient capture of monoclonal antibodies (mAbs). The resin uses the well-established SuRe protein A ligand with good alkaline stability providing stable processing of mAbs.

• Cost-efficient at low resin utilization, such as in clinical manufacturing.
• Highest DBC of any of our MabSelect SuRe™ resins
• Alkaline-stable, well-established protein A ligand, from the MabSelect SuRe resin, is stable when cleaned with 0.1 M NaOH for 200 cycles and approximately 100 cycles using 0.5 M NaOH.
• Bed volume: 5 mL
• Bed dimensions: 16 x 25 mm
• Bed height: 25 mm
• Column i.d.: 16 mm
• Excellent for use in short bed heights and rapid resin cycling mode to further improve productivity in protein A step.

DBC at different residence times

• The MabSelect™ SuRe 70 resin is based on a high-flow agarose base matrix with an average bead size of 50 μm.
• The optimized porosity enables a fast uptake of mAb, and the DBC is less dependent on residence time (RT) compared to other resins (e.g., MabSelect SuRe™ LX and MabSelect PrismA™ resins).
• The DBC for trastuzumab is higher than 70 g/L in a well-packed column at 6 min RT.

Alkaline-stable Protein A ligand

• As native protein A is sensitive to alkaline conditions, the ligand in MabSelect™ SuRe 70 resin, also used in the MabSelect SuRe™ resin, has been stabilized by engineering one of the IgG-binding domains of protein A.
• The MabSelect™ SuRe 70 resin is stable when cleaned with 0.1 M NaOH for 200 cycles and approximately 100 cycles using 0.5 M NaOH without performance loss.

Formats

• MabSelect™ SuRe 70 resin is available as bulk resin in 25 mL, 200 mL, 1 L, 5 L, and 10 L containers.
• The resin can be ordered in pre-packed column formats such as HiTrap™ columns (1 and 5 mL), HiScreen™ columns, and on request as production scale ReadyToProcess™ columns.
• These formats enable use from lab-scale to commercial-scale manufacturing.

Cytiva's mAb toolbox

• Process volumes, number of batches, target molecule, impurity profile, and cleaning protocols influence which chromatography resin will be most cost-efficient and productive in a given application.
• Cytiva can support you in a discussion about how to set up your process and to make your process more cost efficient.
• And with every Cytiva resin you choose, you have Cytiva quality, an extensive security of supply program, and expertise to go along with it.

Specifications

• Product Type: Column
• Flow Rate: < 20 mL/min.
• For Use With (Application): Antibody Affinity Chromatography
• Quantity: 1 Column