ACROBiosystems ACROBiosystems GFP Fusion Human Mesothelin / MSLN (296-580) Protein, His Tag

Supplier:  ACROBiosystems MSNHG2P425UG

Recombinant Protein;25UG;GFP Fusion Human Mesothelin (296-580), His Tag (MSN-HG2P4) is expressed from human 293 cells (HEK293). It contains AA Glu 296 - Gly 580 (Accession # AAH09272.1).;This protein carries a GFP tag at the N-terminus and a polyhistidine tag at the C-terminus. The protein has a calculated MW of 60.4 kDa. The protein migrates as 70 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.;Immobilized Anti-Human MSLN MAb at 10 μg/mL (100 μL/well) can bind GFP Fusion Human Mesothelin (296-580), His Tag (Cat. No. MSN-HG2P4) with a linear range of 0.039-0.625 μg/mL (QC tested).;Anti-human MSLN MAb (chimeric mouse-human IgG1) captured on CM5 chip via Anti-human IgG Fc antibodies surface, can bind GFP Fusion Human Mesothelin (296-580), His Tag (Cat. No. MSN-HG2P4) with an affinity constant of 0.542 nM as determined in a SPR assay (Biacore T200) (Routinely tested).;2e5 of the MSLN-CAR-293 cells were stained with 100 μL of 3 μg/mL of GFP Fusion Human Mesothelin (296-58

Catalog No. 50-195-7054