Invitrogen™ 53BP1 Recombinant Rabbit Monoclonal Antibody (7H14L12), Alexa Fluor™ Plus 488

Catalog No.	Quantity
70-358-0RP488	50 μL
50-312-6255	20 μL

Catalog No. 70-358-0RP488 Supplier Invitrogen™ Supplier No. 703580RP488

Description

Rabbit Recombinant Monoclonal Antibody

Alexa Fluor™ Plus recombinant antibodies are conjugated using new, proprietary dye chemistry so you can generate stunning data. Alexa Fluor™ Plus antibodies represent an advancement in fluorescent conjugate technology. Alexa Fluor™ Plus antibodies provide brighter signal compared to leading Alexa Fluor™ antibodies, providing you with better signal-to-noise for your critical experiments. These antibodies show better specificity and lot-to-lot consistency as these are recombinant antibodies, generated by cloning specific genes for the desired antibodies into an expression vector and expressed in vitro. Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining. Applications Tested: This 53BP1 antibody has been tested by immunocytochemistry and flow cytometric analysis of HeLa cells. This may be used for immunocytochemistry at 5 μg/mL and for flow cytometry at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation:...

53BP1 (P53-binding protein 1) plays a critical role in tumor suppression and is a putative substrate of ATM kinase. Upon DNA damage, 53BP1 is phosphorylated and localizes to the presumptive sites of damage, specifically, double-strand breaks. 53BP1 may have a role in checkpoint signaling during mitosis, enhances TP53-mediated transcriptional activation, and participates in DNA repair by maintaining genomic stability.

Specifications

• Antigen: 53BP1
• Applications: Flow Cytometry, Immunocytochemistry
• Classification: Recombinant Monoclonal
• Clone: 7H14L12
• Concentration: 1.0 mg/mL
• Conjugate: Alexa Fluor Plus 488
• Formulation: Proprietary buffer with 0.008% Bromonitrodioxane, 0.008% Methylisothiazolone; pH 6.8
• Gene: TP53BP1
• Gene Accession No.: Q12888
• Gene Alias: 53BP1; FLJ41424; m53BP1; MGC138366; murine p53-binding protein; p202; p53-binding protein 1; p53BP1; TDRD30; TP53; TP53-binding protein 1; Tp53bp1; transformation related protein 53 binding protein 1; transformation related protein 53 binding-protein 1; Trp53bp1; tumor protein 53-binding protein, 1; tumor protein p53 binding protein 1; tumor protein p53-binding protein, 1; tumor suppressor p53-binding protein 1
• Gene Symbols: TP53BP1
• Host Species: Rabbit
• Immunogen: Protein corresponding to human 53BP1 [aa241-aa440].
• Purification Method: Protein A
• Quantity: 50 μL
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 7158
• Target Species: Human
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG