BD Pharmingen™ 647 EdU Click Proliferation Kit, 50 Tests

Description

For research use only

The BD Pharmingen™ 647 EdU Click Proliferation Kit provides a tool for analyzing DNA synthesis in cycling cells. In this method, the thymidine analog EdU (5-ethynyl-2'-deoxyuridine) is incorporated into newly synthesized DNA by cells progressing through the S (DNA synthesis) phase of the cell cycle. EdU can then be detected in fixed and permeabilized cells with a fluorophore-labeled azide. The reaction of the fluorophore-labeled azide and EdU occurs via a copper-catalyzed click reaction between the azide moiety of the fluorophore and the alkyne moiety of EdU. When co-stained with a DNA dye such as 7-AAD, PI, or DAPI, cell populations may be segmented by flow cytometry into the G0/G1-phases (2N DNA content, EdU-negative), S-phase (2N-4N DNA content, EdU-positive), or G2/M-phases (4N DNA content, EdU-negative).

The BD Pharmingen™ 647 EdU Click Proliferation Kit contains Eterneon™ Red 645 Azide, which is excited by the red laser and has an excitation maximum of 643 nm and an emission maximum of 662 nm.

Please note that this kit is provided as Part 1 of 2 (Components A, B, and C) to be stored dry and protected from light at -20°C, and Part 2 of 2 (Components D, E, F, and G) to be stored dry at 2 - 8°C or room temperature. See 'Kit Contents' immediately below for more information.

Specifications

• Quantity: 50 Tests
• Conjugate: Unconjugated
• For Use With (Application): Research Use Only
• For Use With (Equipment): Flow Cytometer

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov