Applied Biosystems™ 7500 Fast Real-Time PCR System, laptop, QST

Catalog No.	Quantity
43-654-64	1 system

Catalog No. 43-654-64 Supplier Applied Biosystems™ Supplier No. 4365464

Description

Includes

Laptop Computer

The 7500 Real-Time PCR System is a powerful platform for labs requiring superior performance and maximum dye versatility.

It is a sophisticated platform for users who require extended capabilities and maximum versatility. The 3rd generation platform features an innovative optical system that enhances sensitivity and lets you access a broader range of fluorophores. The variable excitation capability allows greater sensitivity for longer wavelength (red) dyes.

NEW - 7500 Software v2.0:
Now the easy-to-use StepOne software is available for both the 7500 and 7500 Fast systems with the 7500 Software v2.0 upgrade. The 7500 Software v2.0 incorporates your favorite StepOne Software features, such as multiple plate setup wizards, calculators for standard curve dilution and master mix recipes, QCX3 flags, data filters, and email notification when a run is finished. The 7500 Software v2.0 also includes an enhanced Gene Expression Study package and has a variety of new melting curve protocol options, including multiple peak detection, step and hold temperature control, and customizable ramp rates.

The NEW Gene Expression Study package accommodates large studies better than any other instrument software package:

• Import an unlimited number of Comparative CT (relative quantitation) files to one study
• Group samples and view data both by technical replicate group and biological replicate group
• Use any gene(s) as an endogenous control, including averaging multiple controls together
• Enter known efficiency values to be factored into the RQ results

21 CFR Part 11 Module available:
The SDS v1.4 21CFRp11 Module is a powerful tool for assisting with 21CFRp11 compliance, while still offering the flexibility of user-customizable configuration settings.

• Individual user log-ins can be added for up to four user groups, each group with designated permission settings
• User-customizable permission settings include fourteen system activities, e-signature authority designation, and additional security settings to give you maximum control over your compliance efforts
• Audit trails can be enabled or disabled depending on your traceability needs
• A selection of e-signatures is available to ease e-signatures into your workflow

Supports Many Applications:
Applications include gene expression analysis, pathogen quantitation, SNP genotyping, isothermal and +/- assays utilizing internal positive controls. To facilitate many of these applications, we provide preformulated, ready-to-use, quality-tested, TaqMan™ assays for use with the 7500 system. Now you can reduce your assay optimization efforts.

Upgrade to High-Speed Thermal Cycling:
An optional upgrade to a the 7500 Fast System is available. This 7500 Fast System uses our master mix formulations and enables you to shorten your real-time PCR runs to as little as 30 minutes.

For Research Use Only. Not for use in diagnostics procedures.

This product(s) resides on a Fisher Scientific GSA or VA contract. If you are viewing this page as a nonregistered user, the price(s) displayed is List Price. To view your GSA or VA contract pricing, log in using your account number, or become a registered user by contacting one of our Customer Service teams. You can also view your contract price by searching for this item(s) on GSA Advantage. To place an order, contact Fisher Scientific Customer Service.

Specifications

• Format: 96-well Plate
• Thermal Accuracy: Within 0.5°C (35° to 95°C) of Setpoint/Display Temp. (3 min. after start)
• Sample Ramp Rate: Fast Mode: ± 2.2°C⁄s, Standard mode: ± 1.6°C⁄s
• Sensitivity: Down to 1 Copy of Human RNAse P gene
• Temperature Range (Metric): 4 to 100°C
• Reaction Volume Range: 10-30 μL
• Passive Reference Dye: ROX (Separate Tube), ROX (Pre-mixed)
• Dynamic Range: 9 Logs of Linear Dynamic Range
• Content And Storage: Laptop
• Calibrated Dye: SYBR™ Green I, ROX™, NED™, TAMRA™, JOE™, Texas Red™, Cy5™, VIC™, FAM™, Cy3™
• High-throughput Compatibility: High-throughput Compatible, Multiplexing
• For Use With (Equipment): 7500 Fast System
• Thermal Uniformity: Within 1.0°C (30 s after Clock Start)
• Reaction Speed: Fast, Standard
• Throughput: Manual Loading of four-five 96-well Plates per 8-hr day (Real Time throughput)
• Optics: Tungsten-halogen Lamp, Five-Emission Filters and CCD Camera, Five-Excitation Filters
• Thermal Cycling System: Peltier-based System
• Dimensions: 34 cm (W) x 49 cm (H) x 45 cm (D) (13.39 in. x 19.29 in. x 17.72 in.)
• External Computer: Minitower
• Data Acquisition: Collects Data for all 5 Filters for all Wells regardless of Plate Setup
• Software Compatibility: Primer Express Software, System Software
• Precision: Can distinguish between 5000 and 10000 Template Copies with 99.7% Confidence
• Voltage: 120 V
• Electrical Requirements: 950 W
• Quantity: 1 system
• Weight: 34 kg (75 lb.)

Frequently Asked Questions (FAQs)

What run conditions should I use for the TaqMan® Advanced miRNA assays with the discontinued 7900HT Real-Time PCR System?

We recommend using the same thermal profile as listed for the 7500 and 7500 Fast systems (Table 8) in the TaqMan® Advanced miRNA Assays User Guide: https://assets.thermofisher.com/TFS-Assets%2FLSG%2Fmanuals%2F100027897_TaqManAdv_miRNA_Assays_UG.pdf

Can I import a standard curve for my real-time PCR data into the Design & Analysis Software (v2.x)?

It is possible to import a standard curve into the Design & Analysis Software (v2.x) if you are analyzing results from one of the following Applied Biosystems real-time PCR instruments:
• 7900HT Fast Real-Time PCR System
• 7500/7500 Fast Real-Time PCR System
• StepOne/StepOne Plus Real-Time PCR System
• ViiA 7 Real Time PCR System
• QuantStudio 1 Real-Time PCR System
• QuantStudio 3 & 5 Real-Time PCR System
• QuantStudio 6 & 7 Flex Real-Time PCR System
• QuantStudio 6 & 7 Pro Real-Time PCR Systems
• QuantStudio 12K Flex Real-Time PCR system

Follow the instructions below to import a standard curve for analysis in the Design & Analysis Software (v2.x):
1. Open the data file in the Design & Analysis Software (v2.x).
2. Click on Actions.
3. Click on "Standard Curve Analysis Setting".
4. Select "External Standard Curves" and click on "Import".
5. Browse to the proper .csv file and click "Open".
6. Click "Apply".

What is the difference between a Fast 96-well and standard 96-well block?

There are two main differences between a Fast 96-well and standard 96-well block for the Applied Biosystems real-time PCR systems:
• Reaction well volume: The Fast 96-well blocks have 0.1 mL reaction wells and are compatible with 0.1 mL plates and tubes. The standard 96-well blocks have 0.2 mL reaction wells and are compatible with 0.2 mL plates and tubes.
• Ramp rate: The fast 96-well blocks have a higher maximum ramp rate than standard blocks for the same real-time PCR system. However, both the fast 96-well and standard 96-well blocks are capable of running standard and fast chemistry.

Note: For newer instruments (QuantStudio 3 and 5 Real-Time PCR Systems and later) "0.1 mL" and "0.2 mL" are being used in place of "fast" and "standard" as designations for the two 96-well block formats.

For Research Use Only. Not for use in diagnostic procedures.